BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_C100015 1 BBa_C100015 LIS ORF (B0034 optimized) 2009-07-09T11:00:00Z 2015-08-31T04:07:25Z Synthesis LIS ORF (B0034 optimized) false false _11_ 0 1380 102 Not in stock false None false Matt Gethers BBa_P101115 1 BBa_P101115 LIS ORF with RBS and Term (optimized) 2009-07-09T11:00:00Z 2015-05-08T01:14:12Z Synthesis LIS ORF with RBS and Term (optimized) false false _11_ 0 1380 102 Not in stock false None false Matt Gethers component2010868 1 BBa_B0010 component2010867 1 BBa_C100015 component2010870 1 BBa_B0012 component2010866 1 BBa_B0034 annotation2010868 1 BBa_B0010 range2010868 1 2640 2719 annotation2010867 1 BBa_C100015 range2010867 1 19 2631 annotation2010870 1 BBa_B0012 range2010870 1 2728 2768 annotation2010866 1 BBa_B0034 range2010866 1 1 12 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_P101115_sequence 1 aaagaggagaaatactagatgcagctgattacgaacttcagcagcagctctagcgaactgcaattcctggttgacaaagttaaacgtgaatctctgtcctcctcctccagcaacactcaaaacctgtttctgtccacctctccgtacgacactgcatggctggcgctgatcccgcaccctcaccaccaccaccatcacggccgtccaatgtttgagaaatgcctgcaatggatcctgcacaaccagaccccgcaaggcttttgggcagctgctggcgacaacatctccgatactgacgacgatgtaaccctggactgcctgctgagcaccctggcctgtctggtcgcgctgaaacgctggcagctggctccggatatgatccataaaggtctggagtttgtgaatcgcaacaccgagcgtctggtgatgaaacagaaaccgtccgatgttccgcgctggtttactatcatgttcccggctatgctggagctggctggcgcttctagcctgcgtgtagatttctctgaaaacctgaaccgtatcctggttgaactgagccagaatcgtgacgatatcctgactcgtgaagaagtggatgaaaagaaacagtatagcccgctgctgctgttcctggaagcgctgccggctcagtcctacgacaatgatgtgctgaagcagattattgataagaacctgagcaacgacggttccctgctgcaatctccgtctgcgactgcccgtgcttatatgatcactggtaatacccgttgcctgtcttacctgcattctctgaccaactcttgcagcaacggcggtgttccatccttctacccggtggatgacgacctgcatgatctggttatggttaaccaactgacccgtagcggtctgactgaacacctgattccagaaatcgatcacctgctgctgaaagtgcagaaaaactacaaatacaagaaagcgagcccgaaaagcctgtacagcattgctgctgagctgtaccgcgattctctggccttctggctgctgcgtgtcaacaaccactgggtttctccgtctatcttttgctggtttctggatgacgacgaaattcgtgaccacattgaaaccaactacgaggaatttgctgccgtgctgctgaatgtttaccgtgcaacggacctgatgttttccggtgaggtgcagctggtcgaagcccgttccttcgcgactaaaaacctggaaaaaattctggcaaccggtaacatccataaaactaacgcggatatttctagctctctgcacaaaatgattgagcacgagctgcgcgtcccgtggacggcgcgtatggaccatgtcgaaaaccgcatttggatcgaagagatcgcatccagcgcactgtggttcggtaaaagctcttacctgcgcctgagctgcttccataaaatgagcctgcaacagctggcggttaaaaactatactctgcgtcagctggtgtatcgtgacgaactggcggaagttgaacgctggtccaaagagcgtggcctgtgcgacatgggtttctgccgtgagaaaaccggctactgttactacgcttttgctgcaagcacctgtctgccgtggagctctgacgtccgtctggttctgactaaagcagctgttgtaattaccgtggcagacgacttcttcgatgtggagggtagcatggtggacctggaaaaactgacggacgcagttcgtcgttgggatgcggaaggcctgggctctcactctaagactatcttcgaagctctggatgacctggtaaacgaagtgcgcctgaaatgtttccaacagaacggtcaggacatcaaaaacaacctgcaacaactgtggtacgaaaccttccactcttggctgatggaagctaaatggggcaaaggtctgacctccaaaccgagcgttgacgtgtacctgggtaacgctatgacgtctattgcagctcataccatggtgctgactgcaagctgtctgctgggtcctggttttccagtgcaccagctgtggtcccagcgccgccaccaggatatcacctccctgctgatggtactgactcgcctgctgaacgacatccagtcttacctgaaagaagaagatgaaggtaagattaactacgtgtggatgtacatgattgaaaacaaccaagccagcatcgacgattctgttcgccacgtgcagactatcatcaacgttaaaaagcaggaatttatccaacgtgttctgtccgatcagcactgcaacctgccgaaaagcttcaagcaactgcacttctcttgcctgaaagtgttcaacatgtttttcaactcttctaacatcttcgatacggacaccgatctgctgctggatatccacgaagcgtttgtgtctccacctcaggtcccgaaattcaaaccgcacatcaaaccaccgcaccagctgccggccactctgcaaccgccacaccagccgcagcagattatggttaacaagaagaaagtggaaatggtttacaaatcttatcaccatccgttcaaggtatttaccctgcaaaagaaacagtcctctggtcacggtaccatgaatcctcgtgcgtctatcctggctggtccaaacatcaaactgtgtttcagctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_C100015_sequence 1 atgcagctgattacgaacttcagcagcagctctagcgaactgcaattcctggttgacaaagttaaacgtgaatctctgtcctcctcctccagcaacactcaaaacctgtttctgtccacctctccgtacgacactgcatggctggcgctgatcccgcaccctcaccaccaccaccatcacggccgtccaatgtttgagaaatgcctgcaatggatcctgcacaaccagaccccgcaaggcttttgggcagctgctggcgacaacatctccgatactgacgacgatgtaaccctggactgcctgctgagcaccctggcctgtctggtcgcgctgaaacgctggcagctggctccggatatgatccataaaggtctggagtttgtgaatcgcaacaccgagcgtctggtgatgaaacagaaaccgtccgatgttccgcgctggtttactatcatgttcccggctatgctggagctggctggcgcttctagcctgcgtgtagatttctctgaaaacctgaaccgtatcctggttgaactgagccagaatcgtgacgatatcctgactcgtgaagaagtggatgaaaagaaacagtatagcccgctgctgctgttcctggaagcgctgccggctcagtcctacgacaatgatgtgctgaagcagattattgataagaacctgagcaacgacggttccctgctgcaatctccgtctgcgactgcccgtgcttatatgatcactggtaatacccgttgcctgtcttacctgcattctctgaccaactcttgcagcaacggcggtgttccatccttctacccggtggatgacgacctgcatgatctggttatggttaaccaactgacccgtagcggtctgactgaacacctgattccagaaatcgatcacctgctgctgaaagtgcagaaaaactacaaatacaagaaagcgagcccgaaaagcctgtacagcattgctgctgagctgtaccgcgattctctggccttctggctgctgcgtgtcaacaaccactgggtttctccgtctatcttttgctggtttctggatgacgacgaaattcgtgaccacattgaaaccaactacgaggaatttgctgccgtgctgctgaatgtttaccgtgcaacggacctgatgttttccggtgaggtgcagctggtcgaagcccgttccttcgcgactaaaaacctggaaaaaattctggcaaccggtaacatccataaaactaacgcggatatttctagctctctgcacaaaatgattgagcacgagctgcgcgtcccgtggacggcgcgtatggaccatgtcgaaaaccgcatttggatcgaagagatcgcatccagcgcactgtggttcggtaaaagctcttacctgcgcctgagctgcttccataaaatgagcctgcaacagctggcggttaaaaactatactctgcgtcagctggtgtatcgtgacgaactggcggaagttgaacgctggtccaaagagcgtggcctgtgcgacatgggtttctgccgtgagaaaaccggctactgttactacgcttttgctgcaagcacctgtctgccgtggagctctgacgtccgtctggttctgactaaagcagctgttgtaattaccgtggcagacgacttcttcgatgtggagggtagcatggtggacctggaaaaactgacggacgcagttcgtcgttgggatgcggaaggcctgggctctcactctaagactatcttcgaagctctggatgacctggtaaacgaagtgcgcctgaaatgtttccaacagaacggtcaggacatcaaaaacaacctgcaacaactgtggtacgaaaccttccactcttggctgatggaagctaaatggggcaaaggtctgacctccaaaccgagcgttgacgtgtacctgggtaacgctatgacgtctattgcagctcataccatggtgctgactgcaagctgtctgctgggtcctggttttccagtgcaccagctgtggtcccagcgccgccaccaggatatcacctccctgctgatggtactgactcgcctgctgaacgacatccagtcttacctgaaagaagaagatgaaggtaagattaactacgtgtggatgtacatgattgaaaacaaccaagccagcatcgacgattctgttcgccacgtgcagactatcatcaacgttaaaaagcaggaatttatccaacgtgttctgtccgatcagcactgcaacctgccgaaaagcttcaagcaactgcacttctcttgcctgaaagtgttcaacatgtttttcaactcttctaacatcttcgatacggacaccgatctgctgctggatatccacgaagcgtttgtgtctccacctcaggtcccgaaattcaaaccgcacatcaaaccaccgcaccagctgccggccactctgcaaccgccacaccagccgcagcagattatggttaacaagaagaaagtggaaatggtttacaaatcttatcaccatccgttcaaggtatttaccctgcaaaagaaacagtcctctggtcacggtaccatgaatcctcgtgcgtctatcctggctggtccaaacatcaaactgtgtttcagctaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z