LOCUS       BBa_P101116             1895 bp    DNA     linear       22-Dec-2024
DEFINITION  Oe23 ORF with RBS and Term (optimized)
ACCESSION   BBa_P101116
VERSION     BBa_P101116.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..1758
                     /label=BBa_C100016
     stem_loop       1778..1821
                     /label=stem_loop
     misc_feature    1767..1846
                     /label=BBa_B0010
     terminator      1767..1846
                     /label=BBa_B0010
     stem_loop       1862..1881
                     /label=T7 TE
     misc_feature    1888..1888
                     /label=stop
     polyA_site      1882..1895
                     /label=polya
     misc_feature    1855..1895
                     /label=BBa_B0012
     terminator      1855..1895
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat ggctttctgc atcagctatc tgggtgctgt tctgccgttc
       61 agcttgagcc cacgtactaa attcgccatc ttccataaca cctccaagca tgccgcttat
      121 aaaacttgcc gctggaacat cccacgtgat gtgggtagca ccccgccacc gtccaagctg
      181 catcaggctc tgtgcctgaa tgcgcactct actagctgca tggctgaact gccgatggat
      241 tacgagggca agattcaggg cacccgtcac ctgctgcacc tgaaagacga aaatgaccca
      301 atcgaatccc tgatctttgt cgacgccacc cagcgtctgg gcgtgaacca ccacttccag
      361 aaagagatcg aagaaatcct gcgtaaatct tacgcgacga tgaaatcccc gtccatctgc
      421 aaataccaca ccctgcacga tgtgtccctg ttcttctgtc tgatgcgtca gcatggtcgc
      481 tatgtaagcg cggacgtatt caataacttc aaaggcgaaa gcggccgttt caaagaggaa
      541 ctgaagcgtg acactcgtgg cctggttgaa ctgtacgagg cggcacagct gtcttttgag
      601 ggcgaacgca tcctggatga agcggaaaac ttctctcgtc agatcctgca cggcaacctg
      661 gcaagcatgg aggacaatct gcgccgctct gtgggcaaca agctgcgtta cccattccac
      721 aaatccattg cgcgttttac gggtatcaac tacgatgacg acctgggcgg tatgtacgaa
      781 tggggtaaga ccctgcgtga actggcgctg atggacctgc aagtggaacg ttctgtttac
      841 caggaagagc tgctgcaagt gtctaaatgg tggaatgaac tgggcctgta taagaaactg
      901 accctggctc gcaaccgtcc gttcgaattt tacatgtggt ctatggtaat cctgactgac
      961 tacattaacc tgtccgaaca acgtgtagaa ctgacgaagt ctgttgcatt catctacctg
     1021 attgacgata tctttgatgt gtacggtact ctggacgaac tgatcatctt cactgaagcg
     1081 gttaacaaat gggactattc tgcaacggat actctgccag acaacatgaa aatgtgctat
     1141 atgactctgc tggataccat caacggtact agccagaaaa tctacgaaaa atacggccac
     1201 aatccaattg actctctgaa aactacttgg aagagcctgt gtagcgcctt cctggtagaa
     1261 gcgaaatggt ctgcgtccgg cagcctgccg tctgcaaacg aatatctgga aaacgaaaaa
     1321 gtctcctccg gtgtgtatgt tgtgctgatc catctgttct ttctgatggg cctgggcggt
     1381 accaaccgcg gctctatcga actgaatgac acccgcgaac tgatgtcctc tattgcgatt
     1441 atcgtgcgta tctggaacga tctgggttgt gcgaaaaacg aacaccagaa cggtaaagac
     1501 ggttcttacc tggactgcta caagaaggaa cacatcaacc tgactgcggc gcaggttcac
     1561 gaacatgcgc tggaactggt ggccatcgaa tggaaacgcc tgaacaagga atctttcaac
     1621 ctgaaccacg atagcgtatc ctcttttaag caggcagcgc tgaacttcgc ccgtatggta
     1681 ccgctgatgt atagctatga caacaaccgt cgtggtccgg tcctggaaga atatgtcaaa
     1741 tttatgctgt ctgattaata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
     1801 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     1861 tggctcacct tcgggtgggc ctttctgcgt ttata
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