LOCUS BBa_P111101 1369 bp DNA linear 22-Nov-2024 DEFINITION SAMT Whole construct ACCESSION BBa_P111101 VERSION BBa_P111101.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 81..1232 /label=BBa_C100001 stem_loop 1252..1295 /label=stem_loop misc_feature 1241..1320 /label=BBa_B0010 terminator 1241..1320 /label=BBa_B0010 stem_loop 1336..1355 /label=T7 TE misc_feature 1362..1362 /label=stop polyA_site 1356..1369 /label=polya misc_feature 1329..1369 /label=BBa_B0012 terminator 1329..1369 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaccaaac agacacagaa acagaacaaa cgtatgaaac 121 ttgctcaagt gctgcacatg aacggtggcc tgggtaaatc tagttacgcg tctaacagcc 181 tggttcaacg taaagttatt tcaattacta aaccgatcat cgaagaagcc atgaccgagt 241 tttatacgcg catgctgcct tctccgcaca ccatcagcat cgcggatctg ggctgtagct 301 gcggtccaaa taccctgctg gttgcggctg aactggtaaa aatcatcgtt aaactgcgtc 361 agaaacttga ccgtgaaccc cctccggaat ttcagattca cctgaacgat ttaccaggaa 421 acgacttcaa cagtatcttc cgttacctgc tgccgatgtt ccgcgaagag ctgcgcgagg 481 aaattggcgg tggtgaagaa gcaggccgtc gttgctttgt ttctggggtt ccgggttctt 541 tctacggtcg tttgttcccg acgaagtctc tgcatttcgt gcacagttcc tactccctga 601 tgtggctgtc aaaggttcct gaaggcgtaa aaatgaacaa agaaaacatc tacattgcgt 661 ctacttcccc gcagaacgtc atcaatgcgt actatgaaca gttccaacgc gacttttctt 721 ctttcttaat ttgccgcagt gaggaagtta ttggcggcgg tcgtatggtg ctgaccttcc 781 tgggtcgcaa atccgcgtct gcgcgttcca aagaatgctg ctacatctgg gaactgctaa 841 gcttggcctt gaaacagctg gttctggaag gcgttattga aaaagaaaaa ctgcactctt 901 tccatatccc gcagtacacc ccgtcaccga ccgaagtaaa ggccgaagtt gaaaaagaag 961 gaagctttac ggtaaaccgt ctggaagtct cggaaatcac ctgggcttcc tgcggtaacg 1021 attttcattt gccggaactg gttgcgtctg gcaacgaata caatgttgcg aaatgtatgc 1081 gcagcgttgc tgaaccgctg ctgattgaac actttgggga atctgtgatc gaccgcctgt 1141 ttgaaaaata tcgtgaaatc attttcgacc gcatgtctcg tgaagaaacc aagttcttca 1201 acgtcactat cagtatgact cgccgcgaat aatactagag ccaggcatca aataaaacga 1261 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 1321 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata //