LOCUS       BBa_P111102             1312 bp    DNA     linear       05-Nov-2024
DEFINITION  BAMT Expression Cassette (R0040)
ACCESSION   BBa_P111102
VERSION     BBa_P111102.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..1175
                     /label=BBa_C100002
     stem_loop       1195..1238
                     /label=stem_loop
     misc_feature    1184..1263
                     /label=BBa_B0010
     terminator      1184..1263
                     /label=BBa_B0010
     stem_loop       1279..1298
                     /label=T7 TE
     misc_feature    1305..1305
                     /label=stop
     polyA_site      1299..1312
                     /label=polya
     misc_feature    1272..1312
                     /label=BBa_B0012
     terminator      1272..1312
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaagtga tgaagaaact gctgtgtatg aacattgctg
      121 gtgatggtga gactagctat gcgaacaatt ccggtctgca aaaagtaatg atgtctaaga
      181 gcctgcacgt tctggatgaa accctgaaag acatcatcgg tgaccacgtt ggtttcccga
      241 aatgcttcaa gatgatggac atgggttgtt cttctggtcc taacgctctg ctggtgatgt
      301 ccggcatcat caacacgatc gaagatctgt acaccgaaaa gaacattaac gaactgccgg
      361 aatttgaagt gttcctgaat gatctgccag ataacgactt caacaacctg tttaaactgc
      421 tgagccacga aaacggtaac tgtttcgtat acggtctgcc gggctccttt tatggccgcc
      481 tgctgccgaa gaaaagcctg cacttcgctt acagctccta ctccatccat tggctgagcc
      541 aggttccaga gggtctggaa gacaacaacc gtcagaacat ttacatggcg acggaaagcc
      601 cgccagaggt ctacaaggcg tacgccaaac agtacgaacg cgatttctct acgttcctga
      661 aactgcgcgg cgaagaaatc gtaccgggcg gtcgcatggt tctgactttc aacggtcgca
      721 gcgtagaaga tccgagctct aaagacgatc tggcaatctt taccctgctg gcgaaaaccc
      781 tggttgatat ggttgcggaa ggtctggtaa aaatggacga tctgtactct tttaacattc
      841 cgatctactc tccgtgtacg cgcgaagttg aagcggcaat cctgtctgaa ggctccttta
      901 ccctggatcg cctggaggtt ttccgcgtct gttgggacgc atccgattat actgacgacg
      961 acgatcaaca ggatccgtct atctttggta aacaacgctc cggtaaattt gtggcagatt
     1021 gcgtacgtgc aattaccgaa ccgatgctgg cttcccattt cggtagcacc atcatggacc
     1081 tgctgttcgg taaatacgcg aaaaagatcg ttgaacatct gagcgttgaa aactcctctt
     1141 acttctctat cgttgtcagc ctgtctcgtc gttaatacta gagccaggca tcaaataaaa
     1201 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1261 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
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