LOCUS       BBa_P111103             1387 bp    DNA     linear       05-Nov-2024
DEFINITION  JMT Expression Cassette (R0040,PCR)
ACCESSION   BBa_P111103
VERSION     BBa_P111103.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..1250
                     /label=BBa_C100003
     stem_loop       1270..1313
                     /label=stem_loop
     misc_feature    1259..1338
                     /label=BBa_B0010
     terminator      1259..1338
                     /label=BBa_B0010
     stem_loop       1354..1373
                     /label=T7 TE
     misc_feature    1380..1380
                     /label=stop
     polyA_site      1374..1387
                     /label=polya
     misc_feature    1347..1387
                     /label=BBa_B0012
     terminator      1347..1387
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggaggtaa tgcgagttct tcacatgaac aaaggaaacg
      121 gggaaacaag ttatgccaag aactccaccg ctcagagcaa cataatatct ctaggcagaa
      181 gagtaatgga cgaggccttg aagaagttaa tgatgagcaa ttcagagatt tcgagcattg
      241 gaatcgccga cttaggctgc tcctccggtc cgaacagtct cttgtccatc tccaacatag
      301 ttgacacgat ccacaacttg tgtcctgacc tcgaccgtcc agtccctgag ctcagagtct
      361 ctctcaacga cctccctagc aatgacttca actacatatg tgcttctttg ccagagtttt
      421 acgaccgggt taataataac aaggagggtt tagggttcgg tcgtggagga ggagaatcgt
      481 gttttgtgtc ggccgtccca ggttcgttct acggacgttt gtttcctcgc cggagccttc
      541 actttgtgca ttcttcttct agtttacatt ggttgtctca ggttccatgt cgtgaggcgg
      601 agaaggaaga caggacaata acagctgatt tagaaaacat ggggaaaata tacatatcaa
      661 agacaagtcc taagagtgca cataaagctt atgctcttca attccaaact gatttcttgg
      721 tttttttgag gtcacgatct gaggagttgg tcccgggagg ccgaatggtt ttatcgttcc
      781 ttggtagaag atcactggat cccacaaccg aagagagttg ctatcaatgg gaactcctag
      841 ctcaagctct tatgtccatg gccaaagagg gtatcatcga ggaagagaag atcgatgctt
      901 tcaacgctcc ttactatgct gcgagctccg aagagttgaa aatggtgata gagaaagaag
      961 ggtcattttc gatcgatagg cttgagataa gtccgattga ttgggaaggt gggagtatca
     1021 gtgaggagag ttatgacctt gcaataaggt ccaaacccga agccctagct agtggccgaa
     1081 gagtgtctaa taccataaga gctgtggtcg agccgatgct agaacctact ttcggtgaaa
     1141 atgtgatgga cgagcttttt gaaaggtatg caaagatcgt gggagagtac ttctatgtaa
     1201 gctcgccacg atacgctatt gttattcttt cgctcgttag aaccggttga tactagagcc
     1261 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     1321 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     1381 gtttata
//