LOCUS       BBa_P111104             1387 bp    DNA     linear       05-Nov-2024
DEFINITION  JMT Expression Cassette (R0040, Synth)
ACCESSION   BBa_P111104
VERSION     BBa_P111104.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..1250
                     /label=BBa_C100004
     stem_loop       1270..1313
                     /label=stem_loop
     misc_feature    1259..1338
                     /label=BBa_B0010
     terminator      1259..1338
                     /label=BBa_B0010
     stem_loop       1354..1373
                     /label=T7 TE
     misc_feature    1380..1380
                     /label=stop
     polyA_site      1374..1387
                     /label=polya
     misc_feature    1347..1387
                     /label=BBa_B0012
     terminator      1347..1387
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggaagtaa tgcgcgtact gcacatgaat aagggtaacg
      121 gtgaaacctc ttatgctaag aacagcactg cgcagtccaa tatcatctct ctgggccgtc
      181 gtgttatgga tgaagctctg aagaaactga tgatgtctaa cagcgaaatc tcttccatcg
      241 gtatcgcaga cctgggttgt tcttctggcc caaactccct gctgtctatt agcaacatcg
      301 ttgatacgat tcacaatctg tgcccggatc tggatcgccc agtcccggaa ctgcgtgttt
      361 ctctgaacga tctgccgagc aacgatttta actatatctg cgctagcctg ccggagttct
      421 atgaccgtgt taataacaac aaagaaggtc tgggctttgg tcgtggcggt ggtgaatcct
      481 gttttgtgtc cgcggttccg ggtagcttct acggccgcct gttcccgcgt cgttccctgc
      541 acttcgtcca ctcttcctcc tctctgcatt ggctgtccca ggtaccgtgt cgtgaagcag
      601 aaaaagaaga ccgtaccatt acggcggacc tggaaaacat gggcaagatc tacatctcta
      661 agacctcccc taaaagcgca cacaaggctt acgctctgca atttcagacc gatttcctgg
      721 tgtttctgcg ctcccgttct gaagaactgg tcccgggtgg tcgtatggtc ctgtctttcc
      781 tgggccgtcg ctctctggat cctaccactg aggaatcttg ttatcagtgg gaactgctgg
      841 cccaggcgct gatgtctatg gcgaaagaag gcattattga agaagaaaaa attgacgctt
      901 tcaacgcccc gtactatgca gcgagctccg aagagctgaa aatggtcatc gagaaagagg
      961 gctctttctc catcgatcgc ctggaaatct ctccgatcga ctgggaaggt ggttccatct
     1021 ccgaagaatc ctacgacctg gcaattcgct ctaaaccaga agctctggcg tctggccgcc
     1081 gtgtgagcaa cactattcgt gccgttgtgg aacctatgct ggaaccgacc tttggcgaga
     1141 acgttatgga cgagctgttc gaacgttacg caaagatcgt gggcgagtac ttctacgttt
     1201 cttcccctcg ttacgccatt gtaattctgt ctctggtgcg taccggttga tactagagcc
     1261 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     1321 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     1381 gtttata
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