LOCUS       BBa_P111105             1576 bp    DNA     linear       05-Nov-2024
DEFINITION  SAAT Expression Cassette (R0040)
ACCESSION   BBa_P111105
VERSION     BBa_P111105.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..1439
                     /label=BBa_C100005
     stem_loop       1459..1502
                     /label=stem_loop
     misc_feature    1448..1527
                     /label=BBa_B0010
     terminator      1448..1527
                     /label=BBa_B0010
     stem_loop       1543..1562
                     /label=T7 TE
     misc_feature    1569..1569
                     /label=stop
     polyA_site      1563..1576
                     /label=polya
     misc_feature    1536..1576
                     /label=BBa_B0012
     terminator      1536..1576
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggaaaaaa tcgaagtatc tatcaacagc aaacacacca
      121 ttaagccatc tacgagcagc actccgctgc aaccgtacaa gctgacgctg ctggaccaac
      181 tgacgccgcc ggcatacgtt ccgatcgtct ttttctaccc gatcaccgac cacgacttca
      241 acctgccgca gaccctggct gatctgcgtc aagccctgtc cgaaactctg accctgtact
      301 acccgctgtc tggtcgtgtt aaaaacaacc tgtacatcga tgacttcgag gaaggcgttc
      361 cgtacctgga agcacgcgtt aactgcgaca tgactgattt cctgcgcctg cgtaaaatcg
      421 aatgcctgaa cgaatttgtg ccgattaaac cgttttccat ggaagccatc tccgacgaac
      481 gttacccgct gctgggcgtg caagtaaacg tgttcgactc cggcatcgca attggcgtga
      541 gcgtaagcca taagctgatc gacggcggca ccgctgactg ttttctgaaa tcctggggcg
      601 ctgttttccg tggctgccgc gagaacatta tccacccgtc cctgagcgag gcggcgctgc
      661 tgttcccgcc acgtgacgat ctgccggaga aatatgttga tcagatggag gcgctgtggt
      721 ttgcaggtaa aaaggtggcg acccgccgtt tcgttttcgg cgttaaagcc atttcctcca
      781 tccaggacga ggctaaatcc gaaagcgtac cgaaaccatc tcgtgttcac gctgtaacgg
      841 gcttcctgtg gaagcacctg atcgctgcta gccgcgcact gacttctggt accacctcca
      901 cccgcctgag cattgcggct caggctgtta acctgcgcac gcgtatgaac atggaaaccg
      961 tgctggataa cgccacgggt aacctgtttt ggtgggcaca ggctattctg gaactgagcc
     1021 acacgacccc ggagattagc gacctgaaac tgtgcgacct ggtgaacctg ctgaacggca
     1081 gcgttaaaca atgcaacggt gattacttcg aaactttcaa aggcaaagaa ggttacggcc
     1141 gcatgtgcga atacctggat ttccaacgca cgatgtcttc tatggaaccg gcgccagata
     1201 tttatctgtt ctcctcctgg accaacttct ttaacccact ggatttcggt tggggccgta
     1261 cctcttggat cggcgtggcg ggcaaaatcg agagcgcgtc ttgcaaattc attattctgg
     1321 ttcctactca gtgcggctct ggcattgaag cgtgggttaa cctggaagaa gaaaagatgg
     1381 cgatgctgga acaggatccg cactttctgg ccctggctag cccgaaaacc ctgatctgat
     1441 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     1501 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
     1561 cctttctgcg tttata
//