LOCUS BBa_P111105 1576 bp DNA linear 25-Nov-2024 DEFINITION SAAT Expression Cassette (R0040) ACCESSION BBa_P111105 VERSION BBa_P111105.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 81..1439 /label=BBa_C100005 stem_loop 1459..1502 /label=stem_loop misc_feature 1448..1527 /label=BBa_B0010 terminator 1448..1527 /label=BBa_B0010 stem_loop 1543..1562 /label=T7 TE misc_feature 1569..1569 /label=stop polyA_site 1563..1576 /label=polya misc_feature 1536..1576 /label=BBa_B0012 terminator 1536..1576 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atggaaaaaa tcgaagtatc tatcaacagc aaacacacca 121 ttaagccatc tacgagcagc actccgctgc aaccgtacaa gctgacgctg ctggaccaac 181 tgacgccgcc ggcatacgtt ccgatcgtct ttttctaccc gatcaccgac cacgacttca 241 acctgccgca gaccctggct gatctgcgtc aagccctgtc cgaaactctg accctgtact 301 acccgctgtc tggtcgtgtt aaaaacaacc tgtacatcga tgacttcgag gaaggcgttc 361 cgtacctgga agcacgcgtt aactgcgaca tgactgattt cctgcgcctg cgtaaaatcg 421 aatgcctgaa cgaatttgtg ccgattaaac cgttttccat ggaagccatc tccgacgaac 481 gttacccgct gctgggcgtg caagtaaacg tgttcgactc cggcatcgca attggcgtga 541 gcgtaagcca taagctgatc gacggcggca ccgctgactg ttttctgaaa tcctggggcg 601 ctgttttccg tggctgccgc gagaacatta tccacccgtc cctgagcgag gcggcgctgc 661 tgttcccgcc acgtgacgat ctgccggaga aatatgttga tcagatggag gcgctgtggt 721 ttgcaggtaa aaaggtggcg acccgccgtt tcgttttcgg cgttaaagcc atttcctcca 781 tccaggacga ggctaaatcc gaaagcgtac cgaaaccatc tcgtgttcac gctgtaacgg 841 gcttcctgtg gaagcacctg atcgctgcta gccgcgcact gacttctggt accacctcca 901 cccgcctgag cattgcggct caggctgtta acctgcgcac gcgtatgaac atggaaaccg 961 tgctggataa cgccacgggt aacctgtttt ggtgggcaca ggctattctg gaactgagcc 1021 acacgacccc ggagattagc gacctgaaac tgtgcgacct ggtgaacctg ctgaacggca 1081 gcgttaaaca atgcaacggt gattacttcg aaactttcaa aggcaaagaa ggttacggcc 1141 gcatgtgcga atacctggat ttccaacgca cgatgtcttc tatggaaccg gcgccagata 1201 tttatctgtt ctcctcctgg accaacttct ttaacccact ggatttcggt tggggccgta 1261 cctcttggat cggcgtggcg ggcaaaatcg agagcgcgtc ttgcaaattc attattctgg 1321 ttcctactca gtgcggctct ggcattgaag cgtgggttaa cctggaagaa gaaaagatgg 1381 cgatgctgga acaggatccg cactttctgg ccctggctag cccgaaaacc ctgatctgat 1441 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 1501 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg 1561 cctttctgcg tttata //