LOCUS BBa_P111106 1519 bp DNA linear 05-Nov-2024 DEFINITION BEAT Expression Cassette (R0040) ACCESSION BBa_P111106 VERSION BBa_P111106.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 81..1382 /label=BBa_C100006 stem_loop 1402..1445 /label=stem_loop misc_feature 1391..1470 /label=BBa_B0010 terminator 1391..1470 /label=BBa_B0010 stem_loop 1486..1505 /label=T7 TE misc_feature 1512..1512 /label=stop polyA_site 1506..1519 /label=polya misc_feature 1479..1519 /label=BBa_B0012 terminator 1479..1519 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaatgtca ctatgcactc caaaaagttg ctgaaaccgt 121 ctattccgac cccgaaccac ctgcaaaaac tgaacctgtc tctgctggac caaatccaga 181 tcccgttcta tgtaggcctg atttttcatt acgaaacgct ctctgataat agcgatatca 241 ctctgtccaa actggaatcc tctctgtctg aaacgctgac gctgtactat cacgtggcag 301 gccgctacaa cggtactgat tgtgtaattg aatgcaatga ccaggggatc gggtacgtag 361 aaaccgcgtt cgatgtggaa ctgcaccagt tcctgctggg cgaagagtct aataacctgg 421 atctgctggt gggtctgtcc ggtttcctgt ctgaaactga aactccgcca ctggctgcta 481 ttcagctgaa catgtttaag tgcggcggtc tggtgatcgg tgcgcagttt aaccacatca 541 tcggcgatat gtttacgatg agcaccttca tgaacagttg ggcaaaggcg tgtcgtgttg 601 gcatcaaaga ggtggcccac ccaacctttg gtctggcgcc gcttatgccg tctgcaaaag 661 ttctgaatat tccaccgccg ccatcattcg aaggtgtcaa atttgtctcc aaacgcttcg 721 tattcaatga aaacgccatt acccgtctgc gtaaggaggc aaccgaagaa gacggcgatg 781 gtgacgacga ccagaagaaa aagcgtccgt ctcgcgtgga tctcgtcacg gcgttcctgt 841 ctaaatctct gatcgagatg gattgcgcga aaaaggaaca gacgaaatca cgcccatctc 901 tgatggtaca tatgatgaac ctgcgtaagc gtacgaagct ggctctggaa aacgacgtat 961 cgggtaactt tttcatcgtt gtgaatgcgg agtctaaaat caccgtggcc ccaaaaatta 1021 ccgatctgac cgaaagcctg ggctccgcgt gcggggaaat catcagtgaa gttgcaaaag 1081 ttgacgatgc agaggttgta tcttctatgg tgcttaacag cgtgcgtgaa ttttactacg 1141 aatggggcaa aggcgaaaag aacgtgttcc tgtacactag ctggtgtcgc ttcccgctgt 1201 acgaagttga cttcggttgg ggcatcccgt ctttagtaga caccaccgcg gtcccgttcg 1261 gcctgattgt tctgatggat gaagcgccgg ctggtgatgg aatcgccgtt cgcgcgtgcc 1321 tgtccgaaca cgacatgatc caattccagc agcaccacca gctgctgtct tacgtttcct 1381 gatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 1441 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 1501 gggcctttct gcgtttata //