LOCUS       BBa_P111106             1519 bp    DNA     linear       26-Dec-2024
DEFINITION  BEAT Expression Cassette (R0040)
ACCESSION   BBa_P111106
VERSION     BBa_P111106.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..1382
                     /label=BBa_C100006
     stem_loop       1402..1445
                     /label=stem_loop
     misc_feature    1391..1470
                     /label=BBa_B0010
     terminator      1391..1470
                     /label=BBa_B0010
     stem_loop       1486..1505
                     /label=T7 TE
     misc_feature    1512..1512
                     /label=stop
     polyA_site      1506..1519
                     /label=polya
     misc_feature    1479..1519
                     /label=BBa_B0012
     terminator      1479..1519
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaatgtca ctatgcactc caaaaagttg ctgaaaccgt
      121 ctattccgac cccgaaccac ctgcaaaaac tgaacctgtc tctgctggac caaatccaga
      181 tcccgttcta tgtaggcctg atttttcatt acgaaacgct ctctgataat agcgatatca
      241 ctctgtccaa actggaatcc tctctgtctg aaacgctgac gctgtactat cacgtggcag
      301 gccgctacaa cggtactgat tgtgtaattg aatgcaatga ccaggggatc gggtacgtag
      361 aaaccgcgtt cgatgtggaa ctgcaccagt tcctgctggg cgaagagtct aataacctgg
      421 atctgctggt gggtctgtcc ggtttcctgt ctgaaactga aactccgcca ctggctgcta
      481 ttcagctgaa catgtttaag tgcggcggtc tggtgatcgg tgcgcagttt aaccacatca
      541 tcggcgatat gtttacgatg agcaccttca tgaacagttg ggcaaaggcg tgtcgtgttg
      601 gcatcaaaga ggtggcccac ccaacctttg gtctggcgcc gcttatgccg tctgcaaaag
      661 ttctgaatat tccaccgccg ccatcattcg aaggtgtcaa atttgtctcc aaacgcttcg
      721 tattcaatga aaacgccatt acccgtctgc gtaaggaggc aaccgaagaa gacggcgatg
      781 gtgacgacga ccagaagaaa aagcgtccgt ctcgcgtgga tctcgtcacg gcgttcctgt
      841 ctaaatctct gatcgagatg gattgcgcga aaaaggaaca gacgaaatca cgcccatctc
      901 tgatggtaca tatgatgaac ctgcgtaagc gtacgaagct ggctctggaa aacgacgtat
      961 cgggtaactt tttcatcgtt gtgaatgcgg agtctaaaat caccgtggcc ccaaaaatta
     1021 ccgatctgac cgaaagcctg ggctccgcgt gcggggaaat catcagtgaa gttgcaaaag
     1081 ttgacgatgc agaggttgta tcttctatgg tgcttaacag cgtgcgtgaa ttttactacg
     1141 aatggggcaa aggcgaaaag aacgtgttcc tgtacactag ctggtgtcgc ttcccgctgt
     1201 acgaagttga cttcggttgg ggcatcccgt ctttagtaga caccaccgcg gtcccgttcg
     1261 gcctgattgt tctgatggat gaagcgccgg ctggtgatgg aatcgccgtt cgcgcgtgcc
     1321 tgtccgaaca cgacatgatc caattccagc agcaccacca gctgctgtct tacgtttcct
     1381 gatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     1441 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     1501 gggcctttct gcgtttata
//