LOCUS       BBa_P111107             1573 bp    DNA     linear       05-Nov-2024
DEFINITION  EHT1 Expression Cassette (R0040)
ACCESSION   BBa_P111107
VERSION     BBa_P111107.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..1436
                     /label=BBa_C100007
     stem_loop       1456..1499
                     /label=stem_loop
     misc_feature    1445..1524
                     /label=BBa_B0010
     terminator      1445..1524
                     /label=BBa_B0010
     stem_loop       1540..1559
                     /label=T7 TE
     misc_feature    1566..1566
                     /label=stop
     polyA_site      1560..1573
                     /label=polya
     misc_feature    1533..1573
                     /label=BBa_B0012
     terminator      1533..1573
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgtcagaag tttccaaatg gccagcaatc aacccattcc
      121 attggggata caatggtaca gtttcgcata ttgtcggtga aaatggttcc attaaactcc
      181 atttaaaaga caacaaggag caagttgatt ttgacgagtt cgctaacaaa tatgtcccaa
      241 cgttgaagaa tggtgcccaa ttcaaattga gtccttactt gttcacaggt attttgcaaa
      301 ctttgtactt aggtgctgct gatttctcta agaaatttcc tgtattctac ggcagggaaa
      361 ttgtcaaatt ctcggatggt ggagtttgca ccgctgactg gctcatagat tcatggaaaa
      421 aggattatga atttgatcaa agtactacga gctttgataa aaaaaaattt gataaagacg
      481 agaaggcgac acatccagaa ggatggcctc gtttacaacc acgtacaagg tacctgaaag
      541 ataatgagtt ggaagaacta cgggaggttg atctacccct agtagttatt ctacatggtc
      601 ttgctggtgg tagtcatgag ccgattataa gatctcttgc tgaaaacctg tctcgcagtg
      661 ggagatttca agtggtcgtc ctaaatacca gaggttgtgc acgttccaaa attaccacca
      721 gaaatttatt tacagcttat cacacaatgg atattcgcga gtttttgcaa agagaaaagc
      781 aaagacatcc agatagaaaa ctatacgctg tgggatgctc ttttggtgct acgatgctgg
      841 caaactatct gggagaagag ggcgataaat cacctttatc cgcagctgct actttgtgca
      901 atccttggga tcttctcctt tcagcaatta ggatgagcca ggattggtgg tcaagaactt
      961 tattttccaa aaatattgcg caattcttaa caagaaccgt tcaggttaat atgggtgaat
     1021 taggagttcc aaatggctct ctccccgatc atcctcccac agtcaagaat ccatctttct
     1081 atatgttcac gcctgaaaat ctaataaagg caaagagctt taaatcgacc cgggaatttg
     1141 atgaagtgta cactgcgcct gctttaggct tcccaaatgc tatggagtat tataaagcgg
     1201 ccagctcaat aaacagagtt gatacaattc gggttcctac ccttgttatc aattccaggg
     1261 atgatcctgt tgtcggccca gatcaaccat actcaatcgt ggaaaagaat cctcgtattt
     1321 tgtattgtag aaccgattta ggtggtcatt tagcttacct agataaagac aacaactcgt
     1381 gggctaccaa ggcaattgca gaatttttca ctaagtttga tgaattagtc gtatgatact
     1441 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1501 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1561 ttctgcgttt ata
//