LOCUS       BBa_P111108             1588 bp    DNA     linear       22-Dec-2024
DEFINITION  EEB1 Expression Cassette (R0040)
ACCESSION   BBa_P111108
VERSION     BBa_P111108.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..1451
                     /label=BBa_C100008
     stem_loop       1471..1514
                     /label=stem_loop
     misc_feature    1460..1539
                     /label=BBa_B0010
     terminator      1460..1539
                     /label=BBa_B0010
     stem_loop       1555..1574
                     /label=T7 TE
     misc_feature    1581..1581
                     /label=stop
     polyA_site      1575..1588
                     /label=polya
     misc_feature    1548..1588
                     /label=BBa_B0012
     terminator      1548..1588
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgtttcgtt ctggttatta ccctaccgtt actccatccc
      121 attggggtta taacggcacc gttaaacatg tactgggcga gaaaggcact aagtccctgg
      181 cattccgtga cagcaaacgt cagatcccgc tgcatgaatt tgtgactaaa cacgtaccga
      241 ccctgaaaga cggcgccaac ttccgtctga atagcctgct gttcaccggt tatctccaga
      301 ccctgtattt atccgcaggc gacttcagca agaaattcca ggtgttctat ggccgtgaga
      361 tcattaaatt ctctgacggc ggtgtgtgca ccgctgattg ggttatgccg gaatgggaac
      421 aaacttattc ccttaatgcg gagaaagcgt cgttcaatga aaaacagttc agcaacgacg
      481 aaaaggctac tcacccaaaa ggctggccgc gtctgcaccc gcgtactcgc tacctgtcct
      541 ctgaggaact ggaaaaatgc cactctaaag gttattctta ccccctggtg gtcgtactgc
      601 atggtcttgc cggtggctct cacgaaccgt taatccgtgc actgtccgaa gacctgtcca
      661 aggtgggcga tggtaaattc caagtagttg tgctgaatgc gcgtggctgc tctcgctcca
      721 aagttaccac ccgtcgtatc tttaccgcgt tgcacaccgg cgacgtccgt gagttcctga
      781 accatcagaa ggctctgttc ccgcagcgta aaatctacgc tgtgggcacc tccttcggtg
      841 cggctatgct gactaactat ctgggtgagg aaggtgataa ctgtccgctg aacgctgcgg
      901 tggcactgtc taatccttgg gatttcgtac acacctggga caaactggcg cacgactggt
      961 ggtccaacca tattttctct cgtactctga cccagtttct gacccgcacg gttaaagtaa
     1021 acatgaacga actgcaagta ccggagaact tcgaagtgag tcataagccg actgtcgaaa
     1081 aaccggtctt ctatacttat acacgtgaaa accttgaaaa agctgaaaaa ttcaccgaca
     1141 ttctcgagtt tgacaacctg ttcaccgcgc cgtctatggg tctgccagat ggtctgacct
     1201 actatcgtaa agcttctagt atcaaccgtc tcccgaacat taaaatccca acactgatta
     1261 tcaacgcaac tgatgacccg gtaactggag aaaacgtgat cccgtataag caggcccgtg
     1321 aaaacccatg cgttctgctg tgtgaaaccg atttaggtgg tcacctggcc tacctggata
     1381 acgaaagtaa tagctggctg accaaacaag ctgcggagtt cctgggtagc tttgacgaac
     1441 tggttctgta atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     1501 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     1561 ccttcgggtg ggcctttctg cgtttata
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