BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_P111108 1 BBa_P111108 EEB1 Expression Cassette (R0040) 2009-06-28T11:00:00Z 2015-05-08T01:14:12Z Synthesis/Cloning EEB1 Expression Cassette (R0040) false false _11_ 0 1380 102 Not in stock false None false Matt Gethers component2007332 1 BBa_R0040 component2007340 1 BBa_B0010 component2007342 1 BBa_B0012 component2007339 1 BBa_C100008 component2007338 1 BBa_B0034 annotation2007332 1 BBa_R0040 range2007332 1 1 54 annotation2007340 1 BBa_B0010 range2007340 1 1460 1539 annotation2007338 1 BBa_B0034 range2007338 1 63 74 annotation2007339 1 BBa_C100008 range2007339 1 81 1451 annotation2007342 1 BBa_B0012 range2007342 1 1548 1588 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 BBa_C100008 1 BBa_C100008 ORF of EEB1 odorant enzyme 2009-06-24T11:00:00Z 2015-08-31T04:07:24Z Synthesized ORF of EEB1 odorant enzyme false false _11_ 0 1380 102 Not in stock false Added biobricks prefix and suffix. false Matt Gethers BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986787 1 -10 range1986787 1 43 48 annotation1986786 1 TetR 2 range1986786 1 26 44 annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986783 1 TetR 1 range1986783 1 1 19 annotation1986785 1 -35 range1986785 1 20 25 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_P111108_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgtttcgttctggttattaccctaccgttactccatcccattggggttataacggcaccgttaaacatgtactgggcgagaaaggcactaagtccctggcattccgtgacagcaaacgtcagatcccgctgcatgaatttgtgactaaacacgtaccgaccctgaaagacggcgccaacttccgtctgaatagcctgctgttcaccggttatctccagaccctgtatttatccgcaggcgacttcagcaagaaattccaggtgttctatggccgtgagatcattaaattctctgacggcggtgtgtgcaccgctgattgggttatgccggaatgggaacaaacttattcccttaatgcggagaaagcgtcgttcaatgaaaaacagttcagcaacgacgaaaaggctactcacccaaaaggctggccgcgtctgcacccgcgtactcgctacctgtcctctgaggaactggaaaaatgccactctaaaggttattcttaccccctggtggtcgtactgcatggtcttgccggtggctctcacgaaccgttaatccgtgcactgtccgaagacctgtccaaggtgggcgatggtaaattccaagtagttgtgctgaatgcgcgtggctgctctcgctccaaagttaccacccgtcgtatctttaccgcgttgcacaccggcgacgtccgtgagttcctgaaccatcagaaggctctgttcccgcagcgtaaaatctacgctgtgggcacctccttcggtgcggctatgctgactaactatctgggtgaggaaggtgataactgtccgctgaacgctgcggtggcactgtctaatccttgggatttcgtacacacctgggacaaactggcgcacgactggtggtccaaccatattttctctcgtactctgacccagtttctgacccgcacggttaaagtaaacatgaacgaactgcaagtaccggagaacttcgaagtgagtcataagccgactgtcgaaaaaccggtcttctatacttatacacgtgaaaaccttgaaaaagctgaaaaattcaccgacattctcgagtttgacaacctgttcaccgcgccgtctatgggtctgccagatggtctgacctactatcgtaaagcttctagtatcaaccgtctcccgaacattaaaatcccaacactgattatcaacgcaactgatgacccggtaactggagaaaacgtgatcccgtataagcaggcccgtgaaaacccatgcgttctgctgtgtgaaaccgatttaggtggtcacctggcctacctggataacgaaagtaatagctggctgaccaaacaagctgcggagttcctgggtagctttgacgaactggttctgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0034_sequence 1 aaagaggagaaa BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_C100008_sequence 1 atgtttcgttctggttattaccctaccgttactccatcccattggggttataacggcaccgttaaacatgtactgggcgagaaaggcactaagtccctggcattccgtgacagcaaacgtcagatcccgctgcatgaatttgtgactaaacacgtaccgaccctgaaagacggcgccaacttccgtctgaatagcctgctgttcaccggttatctccagaccctgtatttatccgcaggcgacttcagcaagaaattccaggtgttctatggccgtgagatcattaaattctctgacggcggtgtgtgcaccgctgattgggttatgccggaatgggaacaaacttattcccttaatgcggagaaagcgtcgttcaatgaaaaacagttcagcaacgacgaaaaggctactcacccaaaaggctggccgcgtctgcacccgcgtactcgctacctgtcctctgaggaactggaaaaatgccactctaaaggttattcttaccccctggtggtcgtactgcatggtcttgccggtggctctcacgaaccgttaatccgtgcactgtccgaagacctgtccaaggtgggcgatggtaaattccaagtagttgtgctgaatgcgcgtggctgctctcgctccaaagttaccacccgtcgtatctttaccgcgttgcacaccggcgacgtccgtgagttcctgaaccatcagaaggctctgttcccgcagcgtaaaatctacgctgtgggcacctccttcggtgcggctatgctgactaactatctgggtgaggaaggtgataactgtccgctgaacgctgcggtggcactgtctaatccttgggatttcgtacacacctgggacaaactggcgcacgactggtggtccaaccatattttctctcgtactctgacccagtttctgacccgcacggttaaagtaaacatgaacgaactgcaagtaccggagaacttcgaagtgagtcataagccgactgtcgaaaaaccggtcttctatacttatacacgtgaaaaccttgaaaaagctgaaaaattcaccgacattctcgagtttgacaacctgttcaccgcgccgtctatgggtctgccagatggtctgacctactatcgtaaagcttctagtatcaaccgtctcccgaacattaaaatcccaacactgattatcaacgcaactgatgacccggtaactggagaaaacgtgatcccgtataagcaggcccgtgaaaacccatgcgttctgctgtgtgaaaccgatttaggtggtcacctggcctacctggataacgaaagtaatagctggctgaccaaacaagctgcggagttcctgggtagctttgacgaactggttctgtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z