BBa_R0040
1
p(tetR)
TetR repressible promoter
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210.
Released HQ 2013
Sequence for pTet inverting regulator driven by the TetR protein.</P>
false
true
_1_
0
24
7
In stock
false
<P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P>
true
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
annotation1986783
1
TetR 1
range1986783
1
1
19
annotation1986787
1
-10
range1986787
1
43
48
annotation1986785
1
-35
range1986785
1
20
25
annotation1986784
1
BBa_R0040
range1986784
1
1
54
annotation1986786
1
TetR 2
range1986786
1
26
44
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_P111108
1
BBa_P111108
EEB1 Expression Cassette (R0040)
2009-06-28T11:00:00Z
2015-05-08T01:14:12Z
Synthesis/Cloning
EEB1 Expression Cassette (R0040)
false
false
_11_
0
1380
102
Not in stock
false
None
false
Matt Gethers
component2007342
1
BBa_B0012
component2007339
1
BBa_C100008
component2007332
1
BBa_R0040
component2007338
1
BBa_B0034
component2007340
1
BBa_B0010
annotation2007339
1
BBa_C100008
range2007339
1
81
1451
annotation2007340
1
BBa_B0010
range2007340
1
1460
1539
annotation2007332
1
BBa_R0040
range2007332
1
1
54
annotation2007338
1
BBa_B0034
range2007338
1
63
74
annotation2007342
1
BBa_B0012
range2007342
1
1548
1588
BBa_C100008
1
BBa_C100008
ORF of EEB1 odorant enzyme
2009-06-24T11:00:00Z
2015-08-31T04:07:24Z
Synthesized
ORF of EEB1 odorant enzyme
false
false
_11_
0
1380
102
Not in stock
false
Added biobricks prefix and suffix.
false
Matt Gethers
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1690
1
polya
range1690
1
28
41
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_P111108_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgtttcgttctggttattaccctaccgttactccatcccattggggttataacggcaccgttaaacatgtactgggcgagaaaggcactaagtccctggcattccgtgacagcaaacgtcagatcccgctgcatgaatttgtgactaaacacgtaccgaccctgaaagacggcgccaacttccgtctgaatagcctgctgttcaccggttatctccagaccctgtatttatccgcaggcgacttcagcaagaaattccaggtgttctatggccgtgagatcattaaattctctgacggcggtgtgtgcaccgctgattgggttatgccggaatgggaacaaacttattcccttaatgcggagaaagcgtcgttcaatgaaaaacagttcagcaacgacgaaaaggctactcacccaaaaggctggccgcgtctgcacccgcgtactcgctacctgtcctctgaggaactggaaaaatgccactctaaaggttattcttaccccctggtggtcgtactgcatggtcttgccggtggctctcacgaaccgttaatccgtgcactgtccgaagacctgtccaaggtgggcgatggtaaattccaagtagttgtgctgaatgcgcgtggctgctctcgctccaaagttaccacccgtcgtatctttaccgcgttgcacaccggcgacgtccgtgagttcctgaaccatcagaaggctctgttcccgcagcgtaaaatctacgctgtgggcacctccttcggtgcggctatgctgactaactatctgggtgaggaaggtgataactgtccgctgaacgctgcggtggcactgtctaatccttgggatttcgtacacacctgggacaaactggcgcacgactggtggtccaaccatattttctctcgtactctgacccagtttctgacccgcacggttaaagtaaacatgaacgaactgcaagtaccggagaacttcgaagtgagtcataagccgactgtcgaaaaaccggtcttctatacttatacacgtgaaaaccttgaaaaagctgaaaaattcaccgacattctcgagtttgacaacctgttcaccgcgccgtctatgggtctgccagatggtctgacctactatcgtaaagcttctagtatcaaccgtctcccgaacattaaaatcccaacactgattatcaacgcaactgatgacccggtaactggagaaaacgtgatcccgtataagcaggcccgtgaaaacccatgcgttctgctgtgtgaaaccgatttaggtggtcacctggcctacctggataacgaaagtaatagctggctgaccaaacaagctgcggagttcctgggtagctttgacgaactggttctgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0034_sequence
1
aaagaggagaaa
BBa_R0040_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_C100008_sequence
1
atgtttcgttctggttattaccctaccgttactccatcccattggggttataacggcaccgttaaacatgtactgggcgagaaaggcactaagtccctggcattccgtgacagcaaacgtcagatcccgctgcatgaatttgtgactaaacacgtaccgaccctgaaagacggcgccaacttccgtctgaatagcctgctgttcaccggttatctccagaccctgtatttatccgcaggcgacttcagcaagaaattccaggtgttctatggccgtgagatcattaaattctctgacggcggtgtgtgcaccgctgattgggttatgccggaatgggaacaaacttattcccttaatgcggagaaagcgtcgttcaatgaaaaacagttcagcaacgacgaaaaggctactcacccaaaaggctggccgcgtctgcacccgcgtactcgctacctgtcctctgaggaactggaaaaatgccactctaaaggttattcttaccccctggtggtcgtactgcatggtcttgccggtggctctcacgaaccgttaatccgtgcactgtccgaagacctgtccaaggtgggcgatggtaaattccaagtagttgtgctgaatgcgcgtggctgctctcgctccaaagttaccacccgtcgtatctttaccgcgttgcacaccggcgacgtccgtgagttcctgaaccatcagaaggctctgttcccgcagcgtaaaatctacgctgtgggcacctccttcggtgcggctatgctgactaactatctgggtgaggaaggtgataactgtccgctgaacgctgcggtggcactgtctaatccttgggatttcgtacacacctgggacaaactggcgcacgactggtggtccaaccatattttctctcgtactctgacccagtttctgacccgcacggttaaagtaaacatgaacgaactgcaagtaccggagaacttcgaagtgagtcataagccgactgtcgaaaaaccggtcttctatacttatacacgtgaaaaccttgaaaaagctgaaaaattcaccgacattctcgagtttgacaacctgttcaccgcgccgtctatgggtctgccagatggtctgacctactatcgtaaagcttctagtatcaaccgtctcccgaacattaaaatcccaacactgattatcaacgcaactgatgacccggtaactggagaaaacgtgatcccgtataagcaggcccgtgaaaacccatgcgttctgctgtgtgaaaccgatttaggtggtcacctggcctacctggataacgaaagtaatagctggctgaccaaacaagctgcggagttcctgggtagctttgacgaactggttctgtaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z