LOCUS       BBa_P111110             2830 bp    DNA     linear       25-Nov-2024
DEFINITION  LIS Expression Cassette (R0040)
ACCESSION   BBa_P111110
VERSION     BBa_P111110.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..2693
                     /label=BBa_C100010
     stem_loop       2713..2756
                     /label=stem_loop
     misc_feature    2702..2781
                     /label=BBa_B0010
     terminator      2702..2781
                     /label=BBa_B0010
     stem_loop       2797..2816
                     /label=T7 TE
     misc_feature    2823..2823
                     /label=stop
     polyA_site      2817..2830
                     /label=polya
     misc_feature    2790..2830
                     /label=BBa_B0012
     terminator      2790..2830
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgcagctga ttacgaattt ttcttcttct tctagcgaac
      121 tgcaattcct ggttgacaaa gttaaacgtg aatctctgtc ctcctcctcc agcaacactc
      181 aaaacctgtt tctgtccacc tctccgtacg acactgcatg gctggcgctg atcccgcacc
      241 ctcaccacca ccaccatcac ggccgtccaa tgtttgagaa atgcctgcaa tggatcctgc
      301 acaaccagac cccgcaaggc ttttgggcag ctgctggcga caacatctcc gatactgacg
      361 acgatgtaac cctggactgc ctgctgagca ccctggcctg tctggtcgcg ctgaaacgct
      421 ggcagctggc tccggatatg atccataaag gtctggagtt tgtgaatcgc aacaccgagc
      481 gtctggtgat gaaacagaaa ccgtccgatg ttccgcgctg gtttactatc atgttcccgg
      541 ctatgctgga gctggctggc gcttctagcc tgcgtgtaga tttctctgaa aacctgaacc
      601 gtatcctggt tgaactgagc cagaatcgtg acgatatcct gactcgtgaa gaagtggatg
      661 aaaagaaaca gtatagcccg ctgctgctgt tcctggaagc gctgccggct cagtcctacg
      721 acaatgatgt gctgaagcag attattgata agaacctgag caacgacggt tccctgctgc
      781 aatctccgtc tgcgactgcc cgtgcttata tgatcactgg taatacccgt tgcctgtctt
      841 acctgcattc tctgaccaac tcttgcagca acggcggtgt tccatccttc tacccggtgg
      901 atgacgacct gcatgatctg gttatggtta accaactgac ccgtagcggt ctgactgaac
      961 acctgattcc agaaatcgat cacctgctgc tgaaagtgca gaaaaactac aaatacaaga
     1021 aagcgagccc gaaaagcctg tacagcattg ctgctgagct gtaccgcgat tctctggcct
     1081 tctggctgct gcgtgtcaac aaccactggg tttctccgtc tatcttttgc tggtttctgg
     1141 atgacgacga aattcgtgac cacattgaaa ccaactacga ggaatttgct gccgtgctgc
     1201 tgaatgttta ccgtgcaacg gacctgatgt tttccggtga ggtgcagctg gtcgaagccc
     1261 gttccttcgc gactaaaaac ctggaaaaaa ttctggcaac cggtaacatc cataaaacta
     1321 acgcggatat ttctagctct ctgcacaaaa tgattgagca cgagctgcgc gtcccgtgga
     1381 cggcgcgtat ggaccatgtc gaaaaccgca tttggatcga agagatcgca tccagcgcac
     1441 tgtggttcgg taaaagctct tacctgcgcc tgagctgctt ccataaaatg agcctgcaac
     1501 agctggcggt taaaaactat actctgcgtc agctggtgta tcgtgacgaa ctggcggaag
     1561 ttgaacgctg gtccaaagag cgtggcctgt gcgacatggg tttctgccgt gagaaaaccg
     1621 gctactgtta ctacgctttt gctgcaagca cctgtctgcc gtggagctct gacgtccgtc
     1681 tggttctgac taaagcagct gttgtaatta ccgtggcaga cgacttcttc gatgtggagg
     1741 gtagcatggt ggacctggaa aaactgacgg acgcagttcg tcgttgggat gcggaaggcc
     1801 tgggctctca ctctaagact atcttcgaag ctctggatga cctggtaaac gaagtgcgcc
     1861 tgaaatgttt ccaacagaac ggtcaggaca tcaaaaacaa cctgcaacaa ctgtggtacg
     1921 aaaccttcca ctcttggctg atggaagcta aatggggcaa aggtctgacc tccaaaccga
     1981 gcgttgacgt gtacctgggt aacgctatga cgtctattgc agctcatacc atggtgctga
     2041 ctgcaagctg tctgctgggt cctggttttc cagtgcacca gctgtggtcc cagcgccgcc
     2101 accaggatat cacctccctg ctgatggtac tgactcgcct gctgaacgac atccagtctt
     2161 acctgaaaga agaagatgaa ggtaagatta actacgtgtg gatgtacatg attgaaaaca
     2221 accaagccag catcgacgat tctgttcgcc acgtgcagac tatcatcaac gttaaaaagc
     2281 aggaatttat ccaacgtgtt ctgtccgatc agcactgcaa cctgccgaaa agcttcaagc
     2341 aactgcactt ctcttgcctg aaagtgttca acatgttttt caactcttct aacatcttcg
     2401 atacggacac cgatctgctg ctggatatcc acgaagcgtt tgtgtctcca cctcaggtcc
     2461 cgaaattcaa accgcacatc aaaccaccgc accagctgcc ggccactctg caaccgccac
     2521 accagccgca gcagattatg gttaacaaga agaaagtgga aatggtttac aaatcttatc
     2581 accatccgtt caaggtattt accctgcaaa agaaacagtc ctctggtcac ggtaccatga
     2641 atcctcgtgc gtctatcctg gctggtccaa acatcaaact gtgtttcagc taatactaga
     2701 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     2761 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     2821 tgcgtttata
//