LOCUS       BBa_P111113              946 bp    DNA     linear       01-Jun-2024
DEFINITION  PAD1 Expression Cassette (R0040)
ACCESSION   BBa_P111113
VERSION     BBa_P111113.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..809
                     /label=BBa_C100013
     stem_loop       829..872
                     /label=stem_loop
     misc_feature    818..897
                     /label=BBa_B0010
     terminator      818..897
                     /label=BBa_B0010
     stem_loop       913..932
                     /label=T7 TE
     misc_feature    939..939
                     /label=stop
     polyA_site      933..946
                     /label=polya
     misc_feature    906..946
                     /label=BBa_B0012
     terminator      906..946
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgctcctat ttccaagaag aactaatata gcctttttca
      121 aaacaacagg catttttgct aattttcctt tgctaggtag aaccattaca acttcaccat
      181 ctttccttac acataaactg tcaaaggaag taaccagggc atcaacttcg cctccaagac
      241 caaagagaat tgttgtcgca attactggtg cgactggtgt tgcactggga atcagacttc
      301 tacaagtgct aaaagagttg agcgtagaaa cccatttggt gatttcaaaa tggggtgcag
      361 caacaatgaa atatgaaaca gattgggaac cgcatgacgt ggcggccttg gcaaccaaga
      421 catactctgt tcgtgatgtt tctgcatgca tttcgtccgg atctttccag catgatggta
      481 tgattgttgt gccctgttcc atgaaatcac tagctgctat tagaatcggt tttacagagg
      541 atttaattac aagagctgcc gatgtttcga ttaaagagaa tcgtaagtta ctactggtta
      601 ctcgggaaac ccctttatct tccatccatc ttgaaaacat gttgtcttta tgcagggcag
      661 gtgttataat ttttcctccg gtacctgcgt tttatacaag acccaagagc cttcatgacc
      721 tattagaaca aagtgttggc aggatcctag actgctttgg catccacgct gacacttttc
      781 ctcgttggga aggaataaaa agcaagtaat actagagcca ggcatcaaat aaaacgaaag
      841 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
      901 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//