LOCUS       BBa_P111114             2017 bp    DNA     linear       22-Nov-2024
DEFINITION  LMS complete construct (R0040, B0034 optimized)
ACCESSION   BBa_P111114
VERSION     BBa_P111114.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..1880
                     /label=BBa_C100014
     stem_loop       1900..1943
                     /label=stem_loop
     misc_feature    1889..1968
                     /label=BBa_B0010
     terminator      1889..1968
                     /label=BBa_B0010
     stem_loop       1984..2003
                     /label=T7 TE
     misc_feature    2010..2010
                     /label=stop
     polyA_site      2004..2017
                     /label=polya
     misc_feature    1977..2017
                     /label=BBa_B0012
     terminator      1977..2017
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggcgctga aagttctgtc agtcgcgacc caaatggcga
      121 tcccttctaa cctgactact tgccttcagc cgagccactt caagtccagc ccgaaactgc
      181 tttccagcac caatagtagc agccgtagcc gtctgcgtgt gtactgttct agcagccagc
      241 tgaccaccga acgtcgtagc ggtaactaca acccaagccg ttgggatgtt aactttatcc
      301 aatccctgct gtccgactat aaggaagaca aacatgtgat tcgtgccagt gaactggtga
      361 ccctggttaa aatggagctg gaaaaagaaa ccgatcagat ccgtcaactg gaactgattg
      421 atgacctgca acgtatgggc ttatcggacc acttccagaa tgaatttaaa gaaatcctgt
      481 cttcgatcta tttggaccat cattattaca aaaacccgtt cccgaaagag gaacgtgatc
      541 tgtattctac gtccctggct ttccgcctgc tccgcgagca cggctttcag gtggctcaag
      601 aagttttcga ctccttcaag aacgaagaag gggagttcaa agaatcgctg agcgatgaca
      661 cccgcggctt actccagctg tatgaagcga gcttcctgct gaccgaaggc gaaactaccc
      721 tggaaagcgc acgcgagttt gccaccaaat ttctcgaaga aaaagtaaat gaaggcggtg
      781 tcgatggaga tctgctgacc cgcattgcgt attctctgga catcccgctg cactggcgta
      841 tcaaacgtcc aaacgcgccg gtatggattg agtggtatcg taaacgtccg gacatgaacc
      901 cagtcgttct ggagctggca atcctcgatc tgaacatcgt tcaggcgcag ttccaggaag
      961 aactgaaaga atctttccgt tggtggcgca ataccggttt cgttgaaaaa ctgccattcg
     1021 ctcgcgaccg tctggtggag tgctacttct ggaacactgg aattattgaa ccgcgccagc
     1081 atgcttcagc ccgcattatg atgggtaaag ttaacgctct gattaccgta atcgatgaca
     1141 tttacgacgt atacggcacc ctggaagaac tggaacagtt taccgatctg atccgtcgtt
     1201 gggatatcaa ctcgatcgat cagctgccgg actacatgca gctgtgcttc ctggctctca
     1261 acaacttcgt tgatgacacg tcttatgacg ttatgaagga aaaaggtgta aacgttatcc
     1321 cgtacctgcg ccagagctgg gttgatctgg cggacaagta tatggtcgaa gcacgttggt
     1381 tctacggtgg ccataaaccg tctctggaag agtatctgga gaactcttgg cagagcatca
     1441 gcggcccgtg catgctcacc cacatcttct tccgtgttac cgattctttc accaaagaaa
     1501 ctgtggattc gctgtataaa taccacgatc tggtccgttg gtcttctttc gtgctgcgcc
     1561 tggctgacga cctgggcacc agcgtagaag aagtcagccg tggtgatgta ccgaagtcgc
     1621 tgcaatgtta catgtctgat tacaacgcat ctgaagcgga agctcgcaaa catgtgaaat
     1681 ggttgatcgc tgaggtttgg aaaaagatga acgccgaacg tgtgtccaaa gatagcccat
     1741 tcggtaagga ctttattggt tgcgctgtgg acctgggtcg tatggcacag ctgatgtacc
     1801 acaacggtga tggtcacggc acccagcacc cgatcatcca ccaacagatg acgcgcaccc
     1861 tgtttgaacc gttcgcttaa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     1921 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     1981 actggctcac cttcgggtgg gcctttctgc gtttata
//