LOCUS       BBa_P111116             1957 bp    DNA     linear       29-Nov-2024
DEFINITION  Oe23 complete construct (R0040, B0034 optimized)
ACCESSION   BBa_P111116
VERSION     BBa_P111116.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..1820
                     /label=BBa_C100016
     stem_loop       1840..1883
                     /label=stem_loop
     misc_feature    1829..1908
                     /label=BBa_B0010
     terminator      1829..1908
                     /label=BBa_B0010
     stem_loop       1924..1943
                     /label=T7 TE
     misc_feature    1950..1950
                     /label=stop
     polyA_site      1944..1957
                     /label=polya
     misc_feature    1917..1957
                     /label=BBa_B0012
     terminator      1917..1957
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggctttct gcatcagcta tctgggtgct gttctgccgt
      121 tcagcttgag cccacgtact aaattcgcca tcttccataa cacctccaag catgccgctt
      181 ataaaacttg ccgctggaac atcccacgtg atgtgggtag caccccgcca ccgtccaagc
      241 tgcatcaggc tctgtgcctg aatgcgcact ctactagctg catggctgaa ctgccgatgg
      301 attacgaggg caagattcag ggcacccgtc acctgctgca cctgaaagac gaaaatgacc
      361 caatcgaatc cctgatcttt gtcgacgcca cccagcgtct gggcgtgaac caccacttcc
      421 agaaagagat cgaagaaatc ctgcgtaaat cttacgcgac gatgaaatcc ccgtccatct
      481 gcaaatacca caccctgcac gatgtgtccc tgttcttctg tctgatgcgt cagcatggtc
      541 gctatgtaag cgcggacgta ttcaataact tcaaaggcga aagcggccgt ttcaaagagg
      601 aactgaagcg tgacactcgt ggcctggttg aactgtacga ggcggcacag ctgtcttttg
      661 agggcgaacg catcctggat gaagcggaaa acttctctcg tcagatcctg cacggcaacc
      721 tggcaagcat ggaggacaat ctgcgccgct ctgtgggcaa caagctgcgt tacccattcc
      781 acaaatccat tgcgcgtttt acgggtatca actacgatga cgacctgggc ggtatgtacg
      841 aatggggtaa gaccctgcgt gaactggcgc tgatggacct gcaagtggaa cgttctgttt
      901 accaggaaga gctgctgcaa gtgtctaaat ggtggaatga actgggcctg tataagaaac
      961 tgaccctggc tcgcaaccgt ccgttcgaat tttacatgtg gtctatggta atcctgactg
     1021 actacattaa cctgtccgaa caacgtgtag aactgacgaa gtctgttgca ttcatctacc
     1081 tgattgacga tatctttgat gtgtacggta ctctggacga actgatcatc ttcactgaag
     1141 cggttaacaa atgggactat tctgcaacgg atactctgcc agacaacatg aaaatgtgct
     1201 atatgactct gctggatacc atcaacggta ctagccagaa aatctacgaa aaatacggcc
     1261 acaatccaat tgactctctg aaaactactt ggaagagcct gtgtagcgcc ttcctggtag
     1321 aagcgaaatg gtctgcgtcc ggcagcctgc cgtctgcaaa cgaatatctg gaaaacgaaa
     1381 aagtctcctc cggtgtgtat gttgtgctga tccatctgtt ctttctgatg ggcctgggcg
     1441 gtaccaaccg cggctctatc gaactgaatg acacccgcga actgatgtcc tctattgcga
     1501 ttatcgtgcg tatctggaac gatctgggtt gtgcgaaaaa cgaacaccag aacggtaaag
     1561 acggttctta cctggactgc tacaagaagg aacacatcaa cctgactgcg gcgcaggttc
     1621 acgaacatgc gctggaactg gtggccatcg aatggaaacg cctgaacaag gaatctttca
     1681 acctgaacca cgatagcgta tcctctttta agcaggcagc gctgaacttc gcccgtatgg
     1741 taccgctgat gtatagctat gacaacaacc gtcgtggtcc ggtcctggaa gaatatgtca
     1801 aatttatgct gtctgattaa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     1861 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     1921 actggctcac cttcgggtgg gcctttctgc gtttata
//