BBa_C100016 1 BBa_C100016 Oe23 ORF (B0034 optimized) 2009-07-09T11:00:00Z 2015-08-31T04:07:25Z Synthesis Oe23 ORF (B0034 optimized) false false _11_ 0 1380 102 Not in stock false None false Matt Gethers BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986785 1 -35 range1986785 1 20 25 annotation1986787 1 -10 range1986787 1 43 48 annotation1986783 1 TetR 1 range1986783 1 1 19 annotation1986786 1 TetR 2 range1986786 1 26 44 BBa_P111116 1 BBa_P111116 Oe23 complete construct (R0040, B0034 optimized) 2009-07-09T11:00:00Z 2015-05-08T01:14:12Z Synthesis/Cloning Oe23 complete construct (R0040, B0034 optimized) false false _11_ 0 1380 102 Not in stock false None false Matt Gethers component2010996 1 BBa_B0012 component2010992 1 BBa_B0034 component2010993 1 BBa_C100016 component2010994 1 BBa_B0010 component2010986 1 BBa_R0040 annotation2010992 1 BBa_B0034 range2010992 1 63 74 annotation2010993 1 BBa_C100016 range2010993 1 81 1820 annotation2010996 1 BBa_B0012 range2010996 1 1917 1957 annotation2010994 1 BBa_B0010 range2010994 1 1829 1908 annotation2010986 1 BBa_R0040 range2010986 1 1 54 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_C100016_sequence 1 atggctttctgcatcagctatctgggtgctgttctgccgttcagcttgagcccacgtactaaattcgccatcttccataacacctccaagcatgccgcttataaaacttgccgctggaacatcccacgtgatgtgggtagcaccccgccaccgtccaagctgcatcaggctctgtgcctgaatgcgcactctactagctgcatggctgaactgccgatggattacgagggcaagattcagggcacccgtcacctgctgcacctgaaagacgaaaatgacccaatcgaatccctgatctttgtcgacgccacccagcgtctgggcgtgaaccaccacttccagaaagagatcgaagaaatcctgcgtaaatcttacgcgacgatgaaatccccgtccatctgcaaataccacaccctgcacgatgtgtccctgttcttctgtctgatgcgtcagcatggtcgctatgtaagcgcggacgtattcaataacttcaaaggcgaaagcggccgtttcaaagaggaactgaagcgtgacactcgtggcctggttgaactgtacgaggcggcacagctgtcttttgagggcgaacgcatcctggatgaagcggaaaacttctctcgtcagatcctgcacggcaacctggcaagcatggaggacaatctgcgccgctctgtgggcaacaagctgcgttacccattccacaaatccattgcgcgttttacgggtatcaactacgatgacgacctgggcggtatgtacgaatggggtaagaccctgcgtgaactggcgctgatggacctgcaagtggaacgttctgtttaccaggaagagctgctgcaagtgtctaaatggtggaatgaactgggcctgtataagaaactgaccctggctcgcaaccgtccgttcgaattttacatgtggtctatggtaatcctgactgactacattaacctgtccgaacaacgtgtagaactgacgaagtctgttgcattcatctacctgattgacgatatctttgatgtgtacggtactctggacgaactgatcatcttcactgaagcggttaacaaatgggactattctgcaacggatactctgccagacaacatgaaaatgtgctatatgactctgctggataccatcaacggtactagccagaaaatctacgaaaaatacggccacaatccaattgactctctgaaaactacttggaagagcctgtgtagcgccttcctggtagaagcgaaatggtctgcgtccggcagcctgccgtctgcaaacgaatatctggaaaacgaaaaagtctcctccggtgtgtatgttgtgctgatccatctgttctttctgatgggcctgggcggtaccaaccgcggctctatcgaactgaatgacacccgcgaactgatgtcctctattgcgattatcgtgcgtatctggaacgatctgggttgtgcgaaaaacgaacaccagaacggtaaagacggttcttacctggactgctacaagaaggaacacatcaacctgactgcggcgcaggttcacgaacatgcgctggaactggtggccatcgaatggaaacgcctgaacaaggaatctttcaacctgaaccacgatagcgtatcctcttttaagcaggcagcgctgaacttcgcccgtatggtaccgctgatgtatagctatgacaacaaccgtcgtggtccggtcctggaagaatatgtcaaatttatgctgtctgattaa BBa_P111116_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggctttctgcatcagctatctgggtgctgttctgccgttcagcttgagcccacgtactaaattcgccatcttccataacacctccaagcatgccgcttataaaacttgccgctggaacatcccacgtgatgtgggtagcaccccgccaccgtccaagctgcatcaggctctgtgcctgaatgcgcactctactagctgcatggctgaactgccgatggattacgagggcaagattcagggcacccgtcacctgctgcacctgaaagacgaaaatgacccaatcgaatccctgatctttgtcgacgccacccagcgtctgggcgtgaaccaccacttccagaaagagatcgaagaaatcctgcgtaaatcttacgcgacgatgaaatccccgtccatctgcaaataccacaccctgcacgatgtgtccctgttcttctgtctgatgcgtcagcatggtcgctatgtaagcgcggacgtattcaataacttcaaaggcgaaagcggccgtttcaaagaggaactgaagcgtgacactcgtggcctggttgaactgtacgaggcggcacagctgtcttttgagggcgaacgcatcctggatgaagcggaaaacttctctcgtcagatcctgcacggcaacctggcaagcatggaggacaatctgcgccgctctgtgggcaacaagctgcgttacccattccacaaatccattgcgcgttttacgggtatcaactacgatgacgacctgggcggtatgtacgaatggggtaagaccctgcgtgaactggcgctgatggacctgcaagtggaacgttctgtttaccaggaagagctgctgcaagtgtctaaatggtggaatgaactgggcctgtataagaaactgaccctggctcgcaaccgtccgttcgaattttacatgtggtctatggtaatcctgactgactacattaacctgtccgaacaacgtgtagaactgacgaagtctgttgcattcatctacctgattgacgatatctttgatgtgtacggtactctggacgaactgatcatcttcactgaagcggttaacaaatgggactattctgcaacggatactctgccagacaacatgaaaatgtgctatatgactctgctggataccatcaacggtactagccagaaaatctacgaaaaatacggccacaatccaattgactctctgaaaactacttggaagagcctgtgtagcgccttcctggtagaagcgaaatggtctgcgtccggcagcctgccgtctgcaaacgaatatctggaaaacgaaaaagtctcctccggtgtgtatgttgtgctgatccatctgttctttctgatgggcctgggcggtaccaaccgcggctctatcgaactgaatgacacccgcgaactgatgtcctctattgcgattatcgtgcgtatctggaacgatctgggttgtgcgaaaaacgaacaccagaacggtaaagacggttcttacctggactgctacaagaaggaacacatcaacctgactgcggcgcaggttcacgaacatgcgctggaactggtggccatcgaatggaaacgcctgaacaaggaatctttcaacctgaaccacgatagcgtatcctcttttaagcaggcagcgctgaacttcgcccgtatggtaccgctgatgtatagctatgacaacaaccgtcgtggtccggtcctggaagaatatgtcaaatttatgctgtctgattaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z