LOCUS       BBa_P111117             1963 bp    DNA     linear       26-Nov-2024
DEFINITION  Myrcene Synthase complete construct (R0040, B0034 optimized)
ACCESSION   BBa_P111117
VERSION     BBa_P111117.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..1826
                     /label=BBa_C100017
     stem_loop       1846..1889
                     /label=stem_loop
     misc_feature    1835..1914
                     /label=BBa_B0010
     terminator      1835..1914
                     /label=BBa_B0010
     stem_loop       1930..1949
                     /label=T7 TE
     misc_feature    1956..1956
                     /label=stop
     polyA_site      1950..1963
                     /label=polya
     misc_feature    1923..1963
                     /label=BBa_B0012
     terminator      1923..1963
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggcttttt gtatcagtta cgtaggtgct ctgctgccgt
      121 gttctctgag cacccgtact aaatttgcga tttgccacaa cacgtctaaa ctgcaccgtg
      181 cggcgtataa aacgtcccgc tggaacatcc caggtgacgt tggcagcacc ccgccaccgt
      241 ccaaactgca tcaagcactg tgcctgaacg aacactctct gtcctgcatg gcggaactgc
      301 caatggatta tgagggtaaa atcaaagaga ctcgccacct gctgcacctg aaaggtgaaa
      361 atgacccgat cgaatccctg atttttgtgg acgcgacgct gcgcctgggt gtcaaccatc
      421 acttccagaa agaaatcgaa gaaatcctgc gcaaatccta cgctaccatg aagtccccga
      481 tcatctgcga ataccacacc ctgcacgaag taagcctgtt ctttcgcctg atgcgccagc
      541 acggccgtta cgtaagcgca gacgtattca ataacttcaa gggcgaatcc ggtcgtttca
      601 aagaagaact gaagcgcgat acccgcggtc tggtggagct gtacgaagcc gcacagctgt
      661 ccttcgaagg tgaacgcatt ctggacgaag cggaaaactt ctcccgtcag atcctgcacg
      721 gtaacctggc gggtatggaa gacaacctgc gtcgctctgt tggcaataaa ctgcgttatc
      781 cgtttcacac gtccatcgct cgcttcactg gtcgtaacta cgacgatgat ctgggtggca
      841 tgtacgaatg gggtaagacc ctgcgtgagc tggcgctgat ggatctgcaa gtagaacgtt
      901 ccgtttacca agaagaactg ctgcaagtgt ccaaatggtg gaacgagctg ggtctgtaca
      961 agaaactgaa cctggcgcgc aaccgtccgt ttgaatttta cacctggtct atggttatcc
     1021 tggcagatta tatcaacctg agcgaacagc gcgtggagct gacgaaaagc gttgccttca
     1081 tctatctgat cgacgacatc ttcgacgtgt acggtaccct ggatgaactg atcatcttca
     1141 ccgaagccgt gaataagtgg gactactccg cgaccgatac cctgccagaa aacatgaaga
     1201 tgtgctgtat gacgctgctg gacactatca atggtacttc ccagaaaatc tatgagaaac
     1261 acggttataa ccctatcgat agcctgaaga ccacctggaa aagcctgtgc tctgctttcc
     1321 tggtagaagc taaatggtct gcgtccggca gcctgccttc tgcaaacgaa tacctggaga
     1381 acgagaaagt gtcctctggc gtttacgtgg ttctggtcca tctgttttgt ctgatgggtc
     1441 tgggcggtac ttcccgcggt agcatcgaac tgaacgacac ccaagaactg atgtcttcca
     1501 ttgcaatcat ctttcgtctg tggaatgacc tgggttccgc caagaacgaa caccagaacg
     1561 gtaaagatgg tagctacctg aactgttaca agaaagagca catcaatctg accgctgcac
     1621 aggcgcacga gcacgcgctg gaactggtgg ctattgagtg gaaacgtctg aataaagagt
     1681 cctttaacct gaaccacgac tccgtgagct ccttcaaaca agcagctctg aacctggcac
     1741 gcatggttcc gctgatgtac tcttacgatc acaaccagcg tggtccagta ctggaggagt
     1801 atgtaaagtt tatgctgtct gactaatact agagccaggc atcaaataaa acgaaaggct
     1861 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     1921 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
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