BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986787 1 -10 range1986787 1 43 48 annotation1986785 1 -35 range1986785 1 20 25 annotation1986783 1 TetR 1 range1986783 1 1 19 annotation1986786 1 TetR 2 range1986786 1 26 44 BBa_C100017 1 BBa_C100017 Myrcene Synthase (B0034 optimized) 2009-07-09T11:00:00Z 2015-08-31T04:07:25Z Synthesis Myrcene Synthase (B0034 optimized) false false _11_ 0 1380 102 Not in stock false None false Matt Gethers BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_P111117 1 BBa_P111117 Myrcene Synthase complete construct (R0040, B0034 optimized) 2009-07-09T11:00:00Z 2015-05-08T01:14:12Z Synthesis/Cloning Myrcene Synthase complete construct (R0040, B0034 optimized) false false _11_ 0 1380 102 Not in stock false None false Matt Gethers component2011039 1 BBa_B0034 component2011040 1 BBa_C100017 component2011041 1 BBa_B0010 component2011043 1 BBa_B0012 component2011033 1 BBa_R0040 annotation2011041 1 BBa_B0010 range2011041 1 1835 1914 annotation2011033 1 BBa_R0040 range2011033 1 1 54 annotation2011040 1 BBa_C100017 range2011040 1 81 1826 annotation2011039 1 BBa_B0034 range2011039 1 63 74 annotation2011043 1 BBa_B0012 range2011043 1 1923 1963 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_C100017_sequence 1 atggctttttgtatcagttacgtaggtgctctgctgccgtgttctctgagcacccgtactaaatttgcgatttgccacaacacgtctaaactgcaccgtgcggcgtataaaacgtcccgctggaacatcccaggtgacgttggcagcaccccgccaccgtccaaactgcatcaagcactgtgcctgaacgaacactctctgtcctgcatggcggaactgccaatggattatgagggtaaaatcaaagagactcgccacctgctgcacctgaaaggtgaaaatgacccgatcgaatccctgatttttgtggacgcgacgctgcgcctgggtgtcaaccatcacttccagaaagaaatcgaagaaatcctgcgcaaatcctacgctaccatgaagtccccgatcatctgcgaataccacaccctgcacgaagtaagcctgttctttcgcctgatgcgccagcacggccgttacgtaagcgcagacgtattcaataacttcaagggcgaatccggtcgtttcaaagaagaactgaagcgcgatacccgcggtctggtggagctgtacgaagccgcacagctgtccttcgaaggtgaacgcattctggacgaagcggaaaacttctcccgtcagatcctgcacggtaacctggcgggtatggaagacaacctgcgtcgctctgttggcaataaactgcgttatccgtttcacacgtccatcgctcgcttcactggtcgtaactacgacgatgatctgggtggcatgtacgaatggggtaagaccctgcgtgagctggcgctgatggatctgcaagtagaacgttccgtttaccaagaagaactgctgcaagtgtccaaatggtggaacgagctgggtctgtacaagaaactgaacctggcgcgcaaccgtccgtttgaattttacacctggtctatggttatcctggcagattatatcaacctgagcgaacagcgcgtggagctgacgaaaagcgttgccttcatctatctgatcgacgacatcttcgacgtgtacggtaccctggatgaactgatcatcttcaccgaagccgtgaataagtgggactactccgcgaccgataccctgccagaaaacatgaagatgtgctgtatgacgctgctggacactatcaatggtacttcccagaaaatctatgagaaacacggttataaccctatcgatagcctgaagaccacctggaaaagcctgtgctctgctttcctggtagaagctaaatggtctgcgtccggcagcctgccttctgcaaacgaatacctggagaacgagaaagtgtcctctggcgtttacgtggttctggtccatctgttttgtctgatgggtctgggcggtacttcccgcggtagcatcgaactgaacgacacccaagaactgatgtcttccattgcaatcatctttcgtctgtggaatgacctgggttccgccaagaacgaacaccagaacggtaaagatggtagctacctgaactgttacaagaaagagcacatcaatctgaccgctgcacaggcgcacgagcacgcgctggaactggtggctattgagtggaaacgtctgaataaagagtcctttaacctgaaccacgactccgtgagctccttcaaacaagcagctctgaacctggcacgcatggttccgctgatgtactcttacgatcacaaccagcgtggtccagtactggaggagtatgtaaagtttatgctgtctgactaa BBa_B0034_sequence 1 aaagaggagaaa BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_P111117_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggctttttgtatcagttacgtaggtgctctgctgccgtgttctctgagcacccgtactaaatttgcgatttgccacaacacgtctaaactgcaccgtgcggcgtataaaacgtcccgctggaacatcccaggtgacgttggcagcaccccgccaccgtccaaactgcatcaagcactgtgcctgaacgaacactctctgtcctgcatggcggaactgccaatggattatgagggtaaaatcaaagagactcgccacctgctgcacctgaaaggtgaaaatgacccgatcgaatccctgatttttgtggacgcgacgctgcgcctgggtgtcaaccatcacttccagaaagaaatcgaagaaatcctgcgcaaatcctacgctaccatgaagtccccgatcatctgcgaataccacaccctgcacgaagtaagcctgttctttcgcctgatgcgccagcacggccgttacgtaagcgcagacgtattcaataacttcaagggcgaatccggtcgtttcaaagaagaactgaagcgcgatacccgcggtctggtggagctgtacgaagccgcacagctgtccttcgaaggtgaacgcattctggacgaagcggaaaacttctcccgtcagatcctgcacggtaacctggcgggtatggaagacaacctgcgtcgctctgttggcaataaactgcgttatccgtttcacacgtccatcgctcgcttcactggtcgtaactacgacgatgatctgggtggcatgtacgaatggggtaagaccctgcgtgagctggcgctgatggatctgcaagtagaacgttccgtttaccaagaagaactgctgcaagtgtccaaatggtggaacgagctgggtctgtacaagaaactgaacctggcgcgcaaccgtccgtttgaattttacacctggtctatggttatcctggcagattatatcaacctgagcgaacagcgcgtggagctgacgaaaagcgttgccttcatctatctgatcgacgacatcttcgacgtgtacggtaccctggatgaactgatcatcttcaccgaagccgtgaataagtgggactactccgcgaccgataccctgccagaaaacatgaagatgtgctgtatgacgctgctggacactatcaatggtacttcccagaaaatctatgagaaacacggttataaccctatcgatagcctgaagaccacctggaaaagcctgtgctctgctttcctggtagaagctaaatggtctgcgtccggcagcctgccttctgcaaacgaatacctggagaacgagaaagtgtcctctggcgtttacgtggttctggtccatctgttttgtctgatgggtctgggcggtacttcccgcggtagcatcgaactgaacgacacccaagaactgatgtcttccattgcaatcatctttcgtctgtggaatgacctgggttccgccaagaacgaacaccagaacggtaaagatggtagctacctgaactgttacaagaaagagcacatcaatctgaccgctgcacaggcgcacgagcacgcgctggaactggtggctattgagtggaaacgtctgaataaagagtcctttaacctgaaccacgactccgtgagctccttcaaacaagcagctctgaacctggcacgcatggttccgctgatgtactcttacgatcacaaccagcgtggtccagtactggaggagtatgtaaagtttatgctgtctgactaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z