LOCUS       BBa_P121101             2376 bp    DNA     linear       25-Nov-2024
DEFINITION  SAMT whole construct (F2620)
ACCESSION   BBa_P121101
VERSION     BBa_P121101.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     terminator      870..998
                     /label=BBa_B0015
     misc_feature    1007..1026
                     /label=LuxR/HSL
     promoter        1026..1031
                     /label=-35
     promoter        1048..1053
                     /label=-10
     misc_feature    1059..1059
                     /label=start
     misc_feature    1007..1061
                     /label=BBa_R0062
     promoter        1007..1061
                     /label=BBa_R0062
     misc_feature    1..1061
                     /label=BBa_F2620
     misc_feature    1074..1077
                     /label=conserved
     RBS             1070..1081
                     /label=BBa_B0034
     CDS             1088..2239
                     /label=BBa_C100001
     stem_loop       2259..2302
                     /label=stem_loop
     misc_feature    2248..2327
                     /label=BBa_B0010
     terminator      2248..2327
                     /label=BBa_B0010
     stem_loop       2343..2362
                     /label=T7 TE
     misc_feature    2369..2369
                     /label=stop
     polyA_site      2363..2376
                     /label=polya
     misc_feature    2336..2376
                     /label=BBa_B0012
     terminator      2336..2376
                     /label=BBa_B0012
     misc_feature    1070..2376
                     /label=BBa_P101101
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt
     1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa
     1081 atactagatg accaaacaga cacagaaaca gaacaaacgt atgaaacttg ctcaagtgct
     1141 gcacatgaac ggtggcctgg gtaaatctag ttacgcgtct aacagcctgg ttcaacgtaa
     1201 agttatttca attactaaac cgatcatcga agaagccatg accgagtttt atacgcgcat
     1261 gctgccttct ccgcacacca tcagcatcgc ggatctgggc tgtagctgcg gtccaaatac
     1321 cctgctggtt gcggctgaac tggtaaaaat catcgttaaa ctgcgtcaga aacttgaccg
     1381 tgaaccccct ccggaatttc agattcacct gaacgattta ccaggaaacg acttcaacag
     1441 tatcttccgt tacctgctgc cgatgttccg cgaagagctg cgcgaggaaa ttggcggtgg
     1501 tgaagaagca ggccgtcgtt gctttgtttc tggggttccg ggttctttct acggtcgttt
     1561 gttcccgacg aagtctctgc atttcgtgca cagttcctac tccctgatgt ggctgtcaaa
     1621 ggttcctgaa ggcgtaaaaa tgaacaaaga aaacatctac attgcgtcta cttccccgca
     1681 gaacgtcatc aatgcgtact atgaacagtt ccaacgcgac ttttcttctt tcttaatttg
     1741 ccgcagtgag gaagttattg gcggcggtcg tatggtgctg accttcctgg gtcgcaaatc
     1801 cgcgtctgcg cgttccaaag aatgctgcta catctgggaa ctgctaagct tggccttgaa
     1861 acagctggtt ctggaaggcg ttattgaaaa agaaaaactg cactctttcc atatcccgca
     1921 gtacaccccg tcaccgaccg aagtaaaggc cgaagttgaa aaagaaggaa gctttacggt
     1981 aaaccgtctg gaagtctcgg aaatcacctg ggcttcctgc ggtaacgatt ttcatttgcc
     2041 ggaactggtt gcgtctggca acgaatacaa tgttgcgaaa tgtatgcgca gcgttgctga
     2101 accgctgctg attgaacact ttggggaatc tgtgatcgac cgcctgtttg aaaaatatcg
     2161 tgaaatcatt ttcgaccgca tgtctcgtga agaaaccaag ttcttcaacg tcactatcag
     2221 tatgactcgc cgcgaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     2281 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     2341 ctggctcacc ttcgggtggg cctttctgcg tttata
//