LOCUS       BBa_P121102             2319 bp    DNA     linear       05-Nov-2024
DEFINITION  BAMT Expression Cassette (F2620)
ACCESSION   BBa_P121102
VERSION     BBa_P121102.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     terminator      870..998
                     /label=BBa_B0015
     misc_feature    1007..1026
                     /label=LuxR/HSL
     promoter        1026..1031
                     /label=-35
     promoter        1048..1053
                     /label=-10
     misc_feature    1059..1059
                     /label=start
     misc_feature    1007..1061
                     /label=BBa_R0062
     promoter        1007..1061
                     /label=BBa_R0062
     misc_feature    1..1061
                     /label=BBa_F2620
     misc_feature    1074..1077
                     /label=conserved
     RBS             1070..1081
                     /label=BBa_B0034
     CDS             1088..2182
                     /label=BBa_C100002
     stem_loop       2202..2245
                     /label=stem_loop
     misc_feature    2191..2270
                     /label=BBa_B0010
     terminator      2191..2270
                     /label=BBa_B0010
     stem_loop       2286..2305
                     /label=T7 TE
     misc_feature    2312..2312
                     /label=stop
     polyA_site      2306..2319
                     /label=polya
     misc_feature    2279..2319
                     /label=BBa_B0012
     terminator      2279..2319
                     /label=BBa_B0012
     misc_feature    1070..2319
                     /label=BBa_P101102
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt
     1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa
     1081 atactagatg aaagtgatga agaaactgct gtgtatgaac attgctggtg atggtgagac
     1141 tagctatgcg aacaattccg gtctgcaaaa agtaatgatg tctaagagcc tgcacgttct
     1201 ggatgaaacc ctgaaagaca tcatcggtga ccacgttggt ttcccgaaat gcttcaagat
     1261 gatggacatg ggttgttctt ctggtcctaa cgctctgctg gtgatgtccg gcatcatcaa
     1321 cacgatcgaa gatctgtaca ccgaaaagaa cattaacgaa ctgccggaat ttgaagtgtt
     1381 cctgaatgat ctgccagata acgacttcaa caacctgttt aaactgctga gccacgaaaa
     1441 cggtaactgt ttcgtatacg gtctgccggg ctccttttat ggccgcctgc tgccgaagaa
     1501 aagcctgcac ttcgcttaca gctcctactc catccattgg ctgagccagg ttccagaggg
     1561 tctggaagac aacaaccgtc agaacattta catggcgacg gaaagcccgc cagaggtcta
     1621 caaggcgtac gccaaacagt acgaacgcga tttctctacg ttcctgaaac tgcgcggcga
     1681 agaaatcgta ccgggcggtc gcatggttct gactttcaac ggtcgcagcg tagaagatcc
     1741 gagctctaaa gacgatctgg caatctttac cctgctggcg aaaaccctgg ttgatatggt
     1801 tgcggaaggt ctggtaaaaa tggacgatct gtactctttt aacattccga tctactctcc
     1861 gtgtacgcgc gaagttgaag cggcaatcct gtctgaaggc tcctttaccc tggatcgcct
     1921 ggaggttttc cgcgtctgtt gggacgcatc cgattatact gacgacgacg atcaacagga
     1981 tccgtctatc tttggtaaac aacgctccgg taaatttgtg gcagattgcg tacgtgcaat
     2041 taccgaaccg atgctggctt cccatttcgg tagcaccatc atggacctgc tgttcggtaa
     2101 atacgcgaaa aagatcgttg aacatctgag cgttgaaaac tcctcttact tctctatcgt
     2161 tgtcagcctg tctcgtcgtt aatactagag ccaggcatca aataaaacga aaggctcagt
     2221 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     2281 acactggctc accttcgggt gggcctttct gcgtttata
//