LOCUS BBa_P121104 2394 bp DNA linear 05-Nov-2024 DEFINITION JMT Expression Cassette (F2620,Synth) ACCESSION BBa_P121104 VERSION BBa_P121104.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..82 /label=prefix misc_feature 254..254 /label=T misc_feature 572..572 /label=A CDS 81..830 /label=luxR misc_feature 81..836 /label=BBa_C0062 misc_feature 837..861 /label=Help:Barcodes CDS 81..836 /label=BBa_C0062 stem_loop 881..924 /label=stem_loop misc_feature 870..949 /label=BBa_B0010 terminator 870..949 /label=BBa_B0010 stem_loop 965..984 /label=T7 TE misc_feature 991..991 /label=stop polyA_site 985..998 /label=polya misc_feature 958..998 /label=BBa_B0012 terminator 958..998 /label=BBa_B0012 terminator 870..998 /label=BBa_B0015 misc_feature 1007..1026 /label=LuxR/HSL promoter 1026..1031 /label=-35 promoter 1048..1053 /label=-10 misc_feature 1059..1059 /label=start misc_feature 1007..1061 /label=BBa_R0062 promoter 1007..1061 /label=BBa_R0062 misc_feature 1..1061 /label=BBa_F2620 misc_feature 1074..1077 /label=conserved RBS 1070..1081 /label=BBa_B0034 CDS 1088..2257 /label=BBa_C100004 stem_loop 2277..2320 /label=stem_loop misc_feature 2266..2345 /label=BBa_B0010 terminator 2266..2345 /label=BBa_B0010 stem_loop 2361..2380 /label=T7 TE misc_feature 2387..2387 /label=stop polyA_site 2381..2394 /label=polya misc_feature 2354..2394 /label=BBa_B0012 terminator 2354..2394 /label=BBa_B0012 misc_feature 1070..2394 /label=BBa_P101104 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt 1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa 1081 atactagatg gaagtaatgc gcgtactgca catgaataag ggtaacggtg aaacctctta 1141 tgctaagaac agcactgcgc agtccaatat catctctctg ggccgtcgtg ttatggatga 1201 agctctgaag aaactgatga tgtctaacag cgaaatctct tccatcggta tcgcagacct 1261 gggttgttct tctggcccaa actccctgct gtctattagc aacatcgttg atacgattca 1321 caatctgtgc ccggatctgg atcgcccagt cccggaactg cgtgtttctc tgaacgatct 1381 gccgagcaac gattttaact atatctgcgc tagcctgccg gagttctatg accgtgttaa 1441 taacaacaaa gaaggtctgg gctttggtcg tggcggtggt gaatcctgtt ttgtgtccgc 1501 ggttccgggt agcttctacg gccgcctgtt cccgcgtcgt tccctgcact tcgtccactc 1561 ttcctcctct ctgcattggc tgtcccaggt accgtgtcgt gaagcagaaa aagaagaccg 1621 taccattacg gcggacctgg aaaacatggg caagatctac atctctaaga cctcccctaa 1681 aagcgcacac aaggcttacg ctctgcaatt tcagaccgat ttcctggtgt ttctgcgctc 1741 ccgttctgaa gaactggtcc cgggtggtcg tatggtcctg tctttcctgg gccgtcgctc 1801 tctggatcct accactgagg aatcttgtta tcagtgggaa ctgctggccc aggcgctgat 1861 gtctatggcg aaagaaggca ttattgaaga agaaaaaatt gacgctttca acgccccgta 1921 ctatgcagcg agctccgaag agctgaaaat ggtcatcgag aaagagggct ctttctccat 1981 cgatcgcctg gaaatctctc cgatcgactg ggaaggtggt tccatctccg aagaatccta 2041 cgacctggca attcgctcta aaccagaagc tctggcgtct ggccgccgtg tgagcaacac 2101 tattcgtgcc gttgtggaac ctatgctgga accgaccttt ggcgagaacg ttatggacga 2161 gctgttcgaa cgttacgcaa agatcgtggg cgagtacttc tacgtttctt cccctcgtta 2221 cgccattgta attctgtctc tggtgcgtac cggttgatac tagagccagg catcaaataa 2281 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg 2341 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata //