LOCUS       BBa_P121104             2394 bp    DNA     linear       25-Nov-2024
DEFINITION  JMT Expression Cassette (F2620,Synth)
ACCESSION   BBa_P121104
VERSION     BBa_P121104.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     terminator      870..998
                     /label=BBa_B0015
     misc_feature    1007..1026
                     /label=LuxR/HSL
     promoter        1026..1031
                     /label=-35
     promoter        1048..1053
                     /label=-10
     misc_feature    1059..1059
                     /label=start
     misc_feature    1007..1061
                     /label=BBa_R0062
     promoter        1007..1061
                     /label=BBa_R0062
     misc_feature    1..1061
                     /label=BBa_F2620
     misc_feature    1074..1077
                     /label=conserved
     RBS             1070..1081
                     /label=BBa_B0034
     CDS             1088..2257
                     /label=BBa_C100004
     stem_loop       2277..2320
                     /label=stem_loop
     misc_feature    2266..2345
                     /label=BBa_B0010
     terminator      2266..2345
                     /label=BBa_B0010
     stem_loop       2361..2380
                     /label=T7 TE
     misc_feature    2387..2387
                     /label=stop
     polyA_site      2381..2394
                     /label=polya
     misc_feature    2354..2394
                     /label=BBa_B0012
     terminator      2354..2394
                     /label=BBa_B0012
     misc_feature    1070..2394
                     /label=BBa_P101104
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt
     1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa
     1081 atactagatg gaagtaatgc gcgtactgca catgaataag ggtaacggtg aaacctctta
     1141 tgctaagaac agcactgcgc agtccaatat catctctctg ggccgtcgtg ttatggatga
     1201 agctctgaag aaactgatga tgtctaacag cgaaatctct tccatcggta tcgcagacct
     1261 gggttgttct tctggcccaa actccctgct gtctattagc aacatcgttg atacgattca
     1321 caatctgtgc ccggatctgg atcgcccagt cccggaactg cgtgtttctc tgaacgatct
     1381 gccgagcaac gattttaact atatctgcgc tagcctgccg gagttctatg accgtgttaa
     1441 taacaacaaa gaaggtctgg gctttggtcg tggcggtggt gaatcctgtt ttgtgtccgc
     1501 ggttccgggt agcttctacg gccgcctgtt cccgcgtcgt tccctgcact tcgtccactc
     1561 ttcctcctct ctgcattggc tgtcccaggt accgtgtcgt gaagcagaaa aagaagaccg
     1621 taccattacg gcggacctgg aaaacatggg caagatctac atctctaaga cctcccctaa
     1681 aagcgcacac aaggcttacg ctctgcaatt tcagaccgat ttcctggtgt ttctgcgctc
     1741 ccgttctgaa gaactggtcc cgggtggtcg tatggtcctg tctttcctgg gccgtcgctc
     1801 tctggatcct accactgagg aatcttgtta tcagtgggaa ctgctggccc aggcgctgat
     1861 gtctatggcg aaagaaggca ttattgaaga agaaaaaatt gacgctttca acgccccgta
     1921 ctatgcagcg agctccgaag agctgaaaat ggtcatcgag aaagagggct ctttctccat
     1981 cgatcgcctg gaaatctctc cgatcgactg ggaaggtggt tccatctccg aagaatccta
     2041 cgacctggca attcgctcta aaccagaagc tctggcgtct ggccgccgtg tgagcaacac
     2101 tattcgtgcc gttgtggaac ctatgctgga accgaccttt ggcgagaacg ttatggacga
     2161 gctgttcgaa cgttacgcaa agatcgtggg cgagtacttc tacgtttctt cccctcgtta
     2221 cgccattgta attctgtctc tggtgcgtac cggttgatac tagagccagg catcaaataa
     2281 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     2341 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//