BBa_R0062 1 lux pR Promoter (luxR & HSL regulated -- lux pR) 2003-01-31T12:00:00Z 2015-05-08T01:14:15Z <em>V. fischeri</em> Released HQ 2013 Promoter activated by LuxR in concert with HSL</p> <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription. false true _1_ 0 24 7 In stock false <P> <P>This promoter is based on the <em>Vibrio fischeri </em>quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <p><img src="<bb_file>Image1.gif</bb_file>" width="614" height="362"><P> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr annotation2046 1 -35 range2046 1 20 25 annotation7070 1 BBa_R0062 range7070 1 1 55 annotation2048 1 start range2048 1 53 53 annotation2047 1 -10 range2047 1 42 47 annotation2045 1 LuxR/HSL range2045 1 1 20 BBa_C100004 1 BBa_C100004 ORF of JMT odorant enzyme (Synthesized) 2009-06-24T11:00:00Z 2015-08-31T04:07:24Z Synthesized ORF of JMT odorant enzyme (Synthesized) false false _11_ 0 1380 102 Not in stock false Added biobricks prefix and suffix. false Matt Gethers BBa_P101104 1 BBa_P101104 JMT RBS.ORF.Term (Synth) 2009-06-28T11:00:00Z 2015-05-08T01:14:11Z Synthesis/Cloning JMT RBS.ORF.Term (Synth) false false _11_ 0 1380 102 Not in stock false None false Matt Gethers component2006892 1 BBa_B0034 component2006893 1 BBa_C100004 component2006896 1 BBa_B0012 component2006894 1 BBa_B0010 annotation2006896 1 BBa_B0012 range2006896 1 1285 1325 annotation2006893 1 BBa_C100004 range2006893 1 19 1188 annotation2006894 1 BBa_B0010 range2006894 1 1197 1276 annotation2006892 1 BBa_B0034 range2006892 1 1 12 BBa_P121104 1 BBa_P121104 JMT Expression Cassette (F2620,Synth) 2009-06-28T11:00:00Z 2015-05-08T01:14:12Z Synthesis/Cloning JMT Expression Cassette (F2620,Synth) false false _11_ 0 1380 102 Not in stock false None false Matt Gethers component2283723 1 BBa_F2620 component2283733 1 BBa_P101104 annotation2283733 1 BBa_P101104 range2283733 1 1070 2394 annotation2283723 1 BBa_F2620 range2283723 1 1 1061 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 BBa_C0062 1 luxr luxR repressor/activator, (no LVA?) 2003-01-31T12:00:00Z 2015-08-31T04:07:23Z <em>V. fischeri</em> <genbank>AF170104</genbank> Released HQ 2013 In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.</p> false true _1_ 0 24 7 In stock false <P> <P>2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in <em>E. coli</em> with codons used at a similar frequency. <P> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr annotation7039 1 BBa_C0062 range7039 1 1 756 annotation1765 1 A range1765 1 492 492 annotation1762 1 prefix range1762 1 1 2 annotation1766 1 luxR range1766 1 1 750 annotation1764 1 T range1764 1 174 174 annotation2213986 1 Help:Barcodes range2213986 1 757 781 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986785 1 -35 range1986785 1 20 25 annotation1986787 1 -10 range1986787 1 43 48 annotation1986786 1 TetR 2 range1986786 1 26 44 annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986783 1 TetR 1 range1986783 1 1 19 BBa_F2620 1 BBa_F2620 3OC₆HSL -> PoPS Receiver 2004-08-08T11:00:00Z 2015-08-31T04:07:27Z Formerly I13270 Released HQ 2013 This device senses the 3OC₆HSL concentration in the media and produces output PoPS according to the transfer function given below. LuxR production is controlled by the tet repressible R0040. Initial characterization work has been done on this device using I13273 as a test device. false true _101_6_ 0 135 6 In stock false <a href="http://model.mit.edu/endipedia/index.php?title=Reciever_Definition#Data_Sheets">Example Data Sheets for F2620</a> true Barry Canton [bcanton@mit.edu] and Anna Labno [labnoa@mit.edu] component2259898 1 BBa_C0062 component2259889 1 BBa_R0040 component2259908 1 BBa_B0015 component2259910 1 BBa_R0062 component2259895 1 BBa_B0034 annotation2259895 1 BBa_B0034 range2259895 1 63 74 annotation2259889 1 BBa_R0040 range2259889 1 1 54 annotation2259910 1 BBa_R0062 range2259910 1 1007 1061 annotation2259908 1 BBa_B0015 range2259908 1 870 998 annotation2259898 1 BBa_C0062 range2259898 1 81 836 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_P121104_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatggaagtaatgcgcgtactgcacatgaataagggtaacggtgaaacctcttatgctaagaacagcactgcgcagtccaatatcatctctctgggccgtcgtgttatggatgaagctctgaagaaactgatgatgtctaacagcgaaatctcttccatcggtatcgcagacctgggttgttcttctggcccaaactccctgctgtctattagcaacatcgttgatacgattcacaatctgtgcccggatctggatcgcccagtcccggaactgcgtgtttctctgaacgatctgccgagcaacgattttaactatatctgcgctagcctgccggagttctatgaccgtgttaataacaacaaagaaggtctgggctttggtcgtggcggtggtgaatcctgttttgtgtccgcggttccgggtagcttctacggccgcctgttcccgcgtcgttccctgcacttcgtccactcttcctcctctctgcattggctgtcccaggtaccgtgtcgtgaagcagaaaaagaagaccgtaccattacggcggacctggaaaacatgggcaagatctacatctctaagacctcccctaaaagcgcacacaaggcttacgctctgcaatttcagaccgatttcctggtgtttctgcgctcccgttctgaagaactggtcccgggtggtcgtatggtcctgtctttcctgggccgtcgctctctggatcctaccactgaggaatcttgttatcagtgggaactgctggcccaggcgctgatgtctatggcgaaagaaggcattattgaagaagaaaaaattgacgctttcaacgccccgtactatgcagcgagctccgaagagctgaaaatggtcatcgagaaagagggctctttctccatcgatcgcctggaaatctctccgatcgactgggaaggtggttccatctccgaagaatcctacgacctggcaattcgctctaaaccagaagctctggcgtctggccgccgtgtgagcaacactattcgtgccgttgtggaacctatgctggaaccgacctttggcgagaacgttatggacgagctgttcgaacgttacgcaaagatcgtgggcgagtacttctacgtttcttcccctcgttacgccattgtaattctgtctctggtgcgtaccggttgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_R0062_sequence 1 acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa BBa_C100004_sequence 1 atggaagtaatgcgcgtactgcacatgaataagggtaacggtgaaacctcttatgctaagaacagcactgcgcagtccaatatcatctctctgggccgtcgtgttatggatgaagctctgaagaaactgatgatgtctaacagcgaaatctcttccatcggtatcgcagacctgggttgttcttctggcccaaactccctgctgtctattagcaacatcgttgatacgattcacaatctgtgcccggatctggatcgcccagtcccggaactgcgtgtttctctgaacgatctgccgagcaacgattttaactatatctgcgctagcctgccggagttctatgaccgtgttaataacaacaaagaaggtctgggctttggtcgtggcggtggtgaatcctgttttgtgtccgcggttccgggtagcttctacggccgcctgttcccgcgtcgttccctgcacttcgtccactcttcctcctctctgcattggctgtcccaggtaccgtgtcgtgaagcagaaaaagaagaccgtaccattacggcggacctggaaaacatgggcaagatctacatctctaagacctcccctaaaagcgcacacaaggcttacgctctgcaatttcagaccgatttcctggtgtttctgcgctcccgttctgaagaactggtcccgggtggtcgtatggtcctgtctttcctgggccgtcgctctctggatcctaccactgaggaatcttgttatcagtgggaactgctggcccaggcgctgatgtctatggcgaaagaaggcattattgaagaagaaaaaattgacgctttcaacgccccgtactatgcagcgagctccgaagagctgaaaatggtcatcgagaaagagggctctttctccatcgatcgcctggaaatctctccgatcgactgggaaggtggttccatctccgaagaatcctacgacctggcaattcgctctaaaccagaagctctggcgtctggccgccgtgtgagcaacactattcgtgccgttgtggaacctatgctggaaccgacctttggcgagaacgttatggacgagctgttcgaacgttacgcaaagatcgtgggcgagtacttctacgtttcttcccctcgttacgccattgtaattctgtctctggtgcgtaccggttga BBa_B0034_sequence 1 aaagaggagaaa BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_P101104_sequence 1 aaagaggagaaatactagatggaagtaatgcgcgtactgcacatgaataagggtaacggtgaaacctcttatgctaagaacagcactgcgcagtccaatatcatctctctgggccgtcgtgttatggatgaagctctgaagaaactgatgatgtctaacagcgaaatctcttccatcggtatcgcagacctgggttgttcttctggcccaaactccctgctgtctattagcaacatcgttgatacgattcacaatctgtgcccggatctggatcgcccagtcccggaactgcgtgtttctctgaacgatctgccgagcaacgattttaactatatctgcgctagcctgccggagttctatgaccgtgttaataacaacaaagaaggtctgggctttggtcgtggcggtggtgaatcctgttttgtgtccgcggttccgggtagcttctacggccgcctgttcccgcgtcgttccctgcacttcgtccactcttcctcctctctgcattggctgtcccaggtaccgtgtcgtgaagcagaaaaagaagaccgtaccattacggcggacctggaaaacatgggcaagatctacatctctaagacctcccctaaaagcgcacacaaggcttacgctctgcaatttcagaccgatttcctggtgtttctgcgctcccgttctgaagaactggtcccgggtggtcgtatggtcctgtctttcctgggccgtcgctctctggatcctaccactgaggaatcttgttatcagtgggaactgctggcccaggcgctgatgtctatggcgaaagaaggcattattgaagaagaaaaaattgacgctttcaacgccccgtactatgcagcgagctccgaagagctgaaaatggtcatcgagaaagagggctctttctccatcgatcgcctggaaatctctccgatcgactgggaaggtggttccatctccgaagaatcctacgacctggcaattcgctctaaaccagaagctctggcgtctggccgccgtgtgagcaacactattcgtgccgttgtggaacctatgctggaaccgacctttggcgagaacgttatggacgagctgttcgaacgttacgcaaagatcgtgggcgagtacttctacgtttcttcccctcgttacgccattgtaattctgtctctggtgcgtaccggttgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_C0062_sequence 1 atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_F2620_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z