LOCUS       BBa_P121105             2583 bp    DNA     linear       05-Nov-2024
DEFINITION  SAAT Expression Cassette (F2620)
ACCESSION   BBa_P121105
VERSION     BBa_P121105.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     terminator      870..998
                     /label=BBa_B0015
     misc_feature    1007..1026
                     /label=LuxR/HSL
     promoter        1026..1031
                     /label=-35
     promoter        1048..1053
                     /label=-10
     misc_feature    1059..1059
                     /label=start
     misc_feature    1007..1061
                     /label=BBa_R0062
     promoter        1007..1061
                     /label=BBa_R0062
     misc_feature    1..1061
                     /label=BBa_F2620
     misc_feature    1074..1077
                     /label=conserved
     RBS             1070..1081
                     /label=BBa_B0034
     CDS             1088..2446
                     /label=BBa_C100005
     stem_loop       2466..2509
                     /label=stem_loop
     misc_feature    2455..2534
                     /label=BBa_B0010
     terminator      2455..2534
                     /label=BBa_B0010
     stem_loop       2550..2569
                     /label=T7 TE
     misc_feature    2576..2576
                     /label=stop
     polyA_site      2570..2583
                     /label=polya
     misc_feature    2543..2583
                     /label=BBa_B0012
     terminator      2543..2583
                     /label=BBa_B0012
     misc_feature    1070..2583
                     /label=BBa_P101105
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt
     1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa
     1081 atactagatg gaaaaaatcg aagtatctat caacagcaaa cacaccatta agccatctac
     1141 gagcagcact ccgctgcaac cgtacaagct gacgctgctg gaccaactga cgccgccggc
     1201 atacgttccg atcgtctttt tctacccgat caccgaccac gacttcaacc tgccgcagac
     1261 cctggctgat ctgcgtcaag ccctgtccga aactctgacc ctgtactacc cgctgtctgg
     1321 tcgtgttaaa aacaacctgt acatcgatga cttcgaggaa ggcgttccgt acctggaagc
     1381 acgcgttaac tgcgacatga ctgatttcct gcgcctgcgt aaaatcgaat gcctgaacga
     1441 atttgtgccg attaaaccgt tttccatgga agccatctcc gacgaacgtt acccgctgct
     1501 gggcgtgcaa gtaaacgtgt tcgactccgg catcgcaatt ggcgtgagcg taagccataa
     1561 gctgatcgac ggcggcaccg ctgactgttt tctgaaatcc tggggcgctg ttttccgtgg
     1621 ctgccgcgag aacattatcc acccgtccct gagcgaggcg gcgctgctgt tcccgccacg
     1681 tgacgatctg ccggagaaat atgttgatca gatggaggcg ctgtggtttg caggtaaaaa
     1741 ggtggcgacc cgccgtttcg ttttcggcgt taaagccatt tcctccatcc aggacgaggc
     1801 taaatccgaa agcgtaccga aaccatctcg tgttcacgct gtaacgggct tcctgtggaa
     1861 gcacctgatc gctgctagcc gcgcactgac ttctggtacc acctccaccc gcctgagcat
     1921 tgcggctcag gctgttaacc tgcgcacgcg tatgaacatg gaaaccgtgc tggataacgc
     1981 cacgggtaac ctgttttggt gggcacaggc tattctggaa ctgagccaca cgaccccgga
     2041 gattagcgac ctgaaactgt gcgacctggt gaacctgctg aacggcagcg ttaaacaatg
     2101 caacggtgat tacttcgaaa ctttcaaagg caaagaaggt tacggccgca tgtgcgaata
     2161 cctggatttc caacgcacga tgtcttctat ggaaccggcg ccagatattt atctgttctc
     2221 ctcctggacc aacttcttta acccactgga tttcggttgg ggccgtacct cttggatcgg
     2281 cgtggcgggc aaaatcgaga gcgcgtcttg caaattcatt attctggttc ctactcagtg
     2341 cggctctggc attgaagcgt gggttaacct ggaagaagaa aagatggcga tgctggaaca
     2401 ggatccgcac tttctggccc tggctagccc gaaaaccctg atctgatact agagccaggc
     2461 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     2521 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     2581 ata
//