LOCUS       BBa_P121106             2526 bp    DNA     linear       22-Dec-2024
DEFINITION  BEAT Expression Cassette (F2620)
ACCESSION   BBa_P121106
VERSION     BBa_P121106.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     terminator      870..998
                     /label=BBa_B0015
     misc_feature    1007..1026
                     /label=LuxR/HSL
     promoter        1026..1031
                     /label=-35
     promoter        1048..1053
                     /label=-10
     misc_feature    1059..1059
                     /label=start
     misc_feature    1007..1061
                     /label=BBa_R0062
     promoter        1007..1061
                     /label=BBa_R0062
     misc_feature    1..1061
                     /label=BBa_F2620
     misc_feature    1074..1077
                     /label=conserved
     RBS             1070..1081
                     /label=BBa_B0034
     CDS             1088..2389
                     /label=BBa_C100006
     stem_loop       2409..2452
                     /label=stem_loop
     misc_feature    2398..2477
                     /label=BBa_B0010
     terminator      2398..2477
                     /label=BBa_B0010
     stem_loop       2493..2512
                     /label=T7 TE
     misc_feature    2519..2519
                     /label=stop
     polyA_site      2513..2526
                     /label=polya
     misc_feature    2486..2526
                     /label=BBa_B0012
     terminator      2486..2526
                     /label=BBa_B0012
     misc_feature    1070..2526
                     /label=BBa_P101106
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt
     1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa
     1081 atactagatg aatgtcacta tgcactccaa aaagttgctg aaaccgtcta ttccgacccc
     1141 gaaccacctg caaaaactga acctgtctct gctggaccaa atccagatcc cgttctatgt
     1201 aggcctgatt tttcattacg aaacgctctc tgataatagc gatatcactc tgtccaaact
     1261 ggaatcctct ctgtctgaaa cgctgacgct gtactatcac gtggcaggcc gctacaacgg
     1321 tactgattgt gtaattgaat gcaatgacca ggggatcggg tacgtagaaa ccgcgttcga
     1381 tgtggaactg caccagttcc tgctgggcga agagtctaat aacctggatc tgctggtggg
     1441 tctgtccggt ttcctgtctg aaactgaaac tccgccactg gctgctattc agctgaacat
     1501 gtttaagtgc ggcggtctgg tgatcggtgc gcagtttaac cacatcatcg gcgatatgtt
     1561 tacgatgagc accttcatga acagttgggc aaaggcgtgt cgtgttggca tcaaagaggt
     1621 ggcccaccca acctttggtc tggcgccgct tatgccgtct gcaaaagttc tgaatattcc
     1681 accgccgcca tcattcgaag gtgtcaaatt tgtctccaaa cgcttcgtat tcaatgaaaa
     1741 cgccattacc cgtctgcgta aggaggcaac cgaagaagac ggcgatggtg acgacgacca
     1801 gaagaaaaag cgtccgtctc gcgtggatct cgtcacggcg ttcctgtcta aatctctgat
     1861 cgagatggat tgcgcgaaaa aggaacagac gaaatcacgc ccatctctga tggtacatat
     1921 gatgaacctg cgtaagcgta cgaagctggc tctggaaaac gacgtatcgg gtaacttttt
     1981 catcgttgtg aatgcggagt ctaaaatcac cgtggcccca aaaattaccg atctgaccga
     2041 aagcctgggc tccgcgtgcg gggaaatcat cagtgaagtt gcaaaagttg acgatgcaga
     2101 ggttgtatct tctatggtgc ttaacagcgt gcgtgaattt tactacgaat ggggcaaagg
     2161 cgaaaagaac gtgttcctgt acactagctg gtgtcgcttc ccgctgtacg aagttgactt
     2221 cggttggggc atcccgtctt tagtagacac caccgcggtc ccgttcggcc tgattgttct
     2281 gatggatgaa gcgccggctg gtgatggaat cgccgttcgc gcgtgcctgt ccgaacacga
     2341 catgatccaa ttccagcagc accaccagct gctgtcttac gtttcctgat actagagcca
     2401 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     2461 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     2521 tttata
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