BBa_R0062
1
lux pR
Promoter (luxR & HSL regulated -- lux pR)
2003-01-31T12:00:00Z
2015-05-08T01:14:15Z
<em>V. fischeri</em>
Released HQ 2013
Promoter activated by LuxR in concert with HSL</p> <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
false
true
_1_
0
24
7
In stock
false
<P> <P>This promoter is based on the <em>Vibrio fischeri </em>quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <p><img src="<bb_file>Image1.gif</bb_file>" width="614" height="362"><P>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
annotation2048
1
start
range2048
1
53
53
annotation2046
1
-35
range2046
1
20
25
annotation7070
1
BBa_R0062
range7070
1
1
55
annotation2047
1
-10
range2047
1
42
47
annotation2045
1
LuxR/HSL
range2045
1
1
20
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916612
1
BBa_B0012
component1916610
1
BBa_B0010
annotation1916610
1
BBa_B0010
range1916610
1
1
80
annotation1916612
1
BBa_B0012
range1916612
1
89
129
BBa_F2620
1
BBa_F2620
3OC<sub>6</sub>HSL -> PoPS Receiver
2004-08-08T11:00:00Z
2015-08-31T04:07:27Z
Formerly I13270
Released HQ 2013
This device senses the 3OC<sub>6</sub>HSL concentration in the media and produces output PoPS according to the transfer function given below. LuxR production is controlled by the tet repressible R0040. Initial characterization work has been done on this device using I13273 as a test device.
false
true
_101_6_
0
135
6
In stock
false
<a href="http://model.mit.edu/endipedia/index.php?title=Reciever_Definition#Data_Sheets">Example Data Sheets for F2620</a>
true
Barry Canton [bcanton@mit.edu] and Anna Labno [labnoa@mit.edu]
component2259898
1
BBa_C0062
component2259889
1
BBa_R0040
component2259908
1
BBa_B0015
component2259910
1
BBa_R0062
component2259895
1
BBa_B0034
annotation2259908
1
BBa_B0015
range2259908
1
870
998
annotation2259889
1
BBa_R0040
range2259889
1
1
54
annotation2259895
1
BBa_B0034
range2259895
1
63
74
annotation2259898
1
BBa_C0062
range2259898
1
81
836
annotation2259910
1
BBa_R0062
range2259910
1
1007
1061
BBa_C0062
1
luxr
luxR repressor/activator, (no LVA?)
2003-01-31T12:00:00Z
2015-08-31T04:07:23Z
<em>V. fischeri</em> <genbank>AF170104</genbank>
Released HQ 2013
In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.</p>
false
true
_1_
0
24
7
In stock
false
<P> <P>2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in <em>E. coli</em> with codons used at a similar frequency. <P>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
annotation7039
1
BBa_C0062
range7039
1
1
756
annotation1762
1
prefix
range1762
1
1
2
annotation2213986
1
Help:Barcodes
range2213986
1
757
781
annotation1765
1
A
range1765
1
492
492
annotation1764
1
T
range1764
1
174
174
annotation1766
1
luxR
range1766
1
1
750
BBa_R0040
1
p(tetR)
TetR repressible promoter
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210.
Released HQ 2013
Sequence for pTet inverting regulator driven by the TetR protein.</P>
false
true
_1_
0
24
7
In stock
false
<P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P>
true
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
annotation1986783
1
TetR 1
range1986783
1
1
19
annotation1986786
1
TetR 2
range1986786
1
26
44
annotation1986784
1
BBa_R0040
range1986784
1
1
54
annotation1986785
1
-35
range1986785
1
20
25
annotation1986787
1
-10
range1986787
1
43
48
BBa_P101106
1
BBa_P101106
BEAT RBS.ORF.Term
2009-06-28T11:00:00Z
2015-05-08T01:14:11Z
Synthesis/Cloning
BEAT RBS.ORF.Term
false
false
_11_
0
1380
102
Not in stock
false
None
false
Matt Gethers
component2006910
1
BBa_B0034
component2006912
1
BBa_B0010
component2006911
1
BBa_C100006
component2006914
1
BBa_B0012
annotation2006910
1
BBa_B0034
range2006910
1
1
12
annotation2006914
1
BBa_B0012
range2006914
1
1417
1457
annotation2006912
1
BBa_B0010
range2006912
1
1329
1408
annotation2006911
1
BBa_C100006
range2006911
1
19
1320
BBa_P121106
1
BBa_P121106
BEAT Expression Cassette (F2620)
2009-06-28T11:00:00Z
2015-05-08T01:14:12Z
Synthesis/Cloning
BEAT Expression Cassette (F2620)
false
false
_11_
0
1380
102
Not in stock
false
None
false
Matt Gethers
component2283805
1
BBa_P101106
component2283795
1
BBa_F2620
annotation2283805
1
BBa_P101106
range2283805
1
1070
2526
annotation2283795
1
BBa_F2620
range2283795
1
1
1061
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_C100006
1
BBa_C100006
ORF for the BEAT odorant enzyme
2009-06-24T11:00:00Z
2015-08-31T04:07:24Z
Synthesized.
ORF for the BEAT odorant enzyme.
false
false
_11_
0
1380
102
Not in stock
false
Added biobricks prefix and suffix.
false
Matt Gethers
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_P101106_sequence
1
aaagaggagaaatactagatgaatgtcactatgcactccaaaaagttgctgaaaccgtctattccgaccccgaaccacctgcaaaaactgaacctgtctctgctggaccaaatccagatcccgttctatgtaggcctgatttttcattacgaaacgctctctgataatagcgatatcactctgtccaaactggaatcctctctgtctgaaacgctgacgctgtactatcacgtggcaggccgctacaacggtactgattgtgtaattgaatgcaatgaccaggggatcgggtacgtagaaaccgcgttcgatgtggaactgcaccagttcctgctgggcgaagagtctaataacctggatctgctggtgggtctgtccggtttcctgtctgaaactgaaactccgccactggctgctattcagctgaacatgtttaagtgcggcggtctggtgatcggtgcgcagtttaaccacatcatcggcgatatgtttacgatgagcaccttcatgaacagttgggcaaaggcgtgtcgtgttggcatcaaagaggtggcccacccaacctttggtctggcgccgcttatgccgtctgcaaaagttctgaatattccaccgccgccatcattcgaaggtgtcaaatttgtctccaaacgcttcgtattcaatgaaaacgccattacccgtctgcgtaaggaggcaaccgaagaagacggcgatggtgacgacgaccagaagaaaaagcgtccgtctcgcgtggatctcgtcacggcgttcctgtctaaatctctgatcgagatggattgcgcgaaaaaggaacagacgaaatcacgcccatctctgatggtacatatgatgaacctgcgtaagcgtacgaagctggctctggaaaacgacgtatcgggtaactttttcatcgttgtgaatgcggagtctaaaatcaccgtggccccaaaaattaccgatctgaccgaaagcctgggctccgcgtgcggggaaatcatcagtgaagttgcaaaagttgacgatgcagaggttgtatcttctatggtgcttaacagcgtgcgtgaattttactacgaatggggcaaaggcgaaaagaacgtgttcctgtacactagctggtgtcgcttcccgctgtacgaagttgacttcggttggggcatcccgtctttagtagacaccaccgcggtcccgttcggcctgattgttctgatggatgaagcgccggctggtgatggaatcgccgttcgcgcgtgcctgtccgaacacgacatgatccaattccagcagcaccaccagctgctgtcttacgtttcctgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_R0062_sequence
1
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
BBa_B0034_sequence
1
aaagaggagaaa
BBa_R0040_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
BBa_C100006_sequence
1
atgaatgtcactatgcactccaaaaagttgctgaaaccgtctattccgaccccgaaccacctgcaaaaactgaacctgtctctgctggaccaaatccagatcccgttctatgtaggcctgatttttcattacgaaacgctctctgataatagcgatatcactctgtccaaactggaatcctctctgtctgaaacgctgacgctgtactatcacgtggcaggccgctacaacggtactgattgtgtaattgaatgcaatgaccaggggatcgggtacgtagaaaccgcgttcgatgtggaactgcaccagttcctgctgggcgaagagtctaataacctggatctgctggtgggtctgtccggtttcctgtctgaaactgaaactccgccactggctgctattcagctgaacatgtttaagtgcggcggtctggtgatcggtgcgcagtttaaccacatcatcggcgatatgtttacgatgagcaccttcatgaacagttgggcaaaggcgtgtcgtgttggcatcaaagaggtggcccacccaacctttggtctggcgccgcttatgccgtctgcaaaagttctgaatattccaccgccgccatcattcgaaggtgtcaaatttgtctccaaacgcttcgtattcaatgaaaacgccattacccgtctgcgtaaggaggcaaccgaagaagacggcgatggtgacgacgaccagaagaaaaagcgtccgtctcgcgtggatctcgtcacggcgttcctgtctaaatctctgatcgagatggattgcgcgaaaaaggaacagacgaaatcacgcccatctctgatggtacatatgatgaacctgcgtaagcgtacgaagctggctctggaaaacgacgtatcgggtaactttttcatcgttgtgaatgcggagtctaaaatcaccgtggccccaaaaattaccgatctgaccgaaagcctgggctccgcgtgcggggaaatcatcagtgaagttgcaaaagttgacgatgcagaggttgtatcttctatggtgcttaacagcgtgcgtgaattttactacgaatggggcaaaggcgaaaagaacgtgttcctgtacactagctggtgtcgcttcccgctgtacgaagttgacttcggttggggcatcccgtctttagtagacaccaccgcggtcccgttcggcctgattgttctgatggatgaagcgccggctggtgatggaatcgccgttcgcgcgtgcctgtccgaacacgacatgatccaattccagcagcaccaccagctgctgtcttacgtttcctga
BBa_P121106_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatgaatgtcactatgcactccaaaaagttgctgaaaccgtctattccgaccccgaaccacctgcaaaaactgaacctgtctctgctggaccaaatccagatcccgttctatgtaggcctgatttttcattacgaaacgctctctgataatagcgatatcactctgtccaaactggaatcctctctgtctgaaacgctgacgctgtactatcacgtggcaggccgctacaacggtactgattgtgtaattgaatgcaatgaccaggggatcgggtacgtagaaaccgcgttcgatgtggaactgcaccagttcctgctgggcgaagagtctaataacctggatctgctggtgggtctgtccggtttcctgtctgaaactgaaactccgccactggctgctattcagctgaacatgtttaagtgcggcggtctggtgatcggtgcgcagtttaaccacatcatcggcgatatgtttacgatgagcaccttcatgaacagttgggcaaaggcgtgtcgtgttggcatcaaagaggtggcccacccaacctttggtctggcgccgcttatgccgtctgcaaaagttctgaatattccaccgccgccatcattcgaaggtgtcaaatttgtctccaaacgcttcgtattcaatgaaaacgccattacccgtctgcgtaaggaggcaaccgaagaagacggcgatggtgacgacgaccagaagaaaaagcgtccgtctcgcgtggatctcgtcacggcgttcctgtctaaatctctgatcgagatggattgcgcgaaaaaggaacagacgaaatcacgcccatctctgatggtacatatgatgaacctgcgtaagcgtacgaagctggctctggaaaacgacgtatcgggtaactttttcatcgttgtgaatgcggagtctaaaatcaccgtggccccaaaaattaccgatctgaccgaaagcctgggctccgcgtgcggggaaatcatcagtgaagttgcaaaagttgacgatgcagaggttgtatcttctatggtgcttaacagcgtgcgtgaattttactacgaatggggcaaaggcgaaaagaacgtgttcctgtacactagctggtgtcgcttcccgctgtacgaagttgacttcggttggggcatcccgtctttagtagacaccaccgcggtcccgttcggcctgattgttctgatggatgaagcgccggctggtgatggaatcgccgttcgcgcgtgcctgtccgaacacgacatgatccaattccagcagcaccaccagctgctgtcttacgtttcctgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_C0062_sequence
1
atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_F2620_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z