LOCUS       BBa_P121107             2580 bp    DNA     linear       26-Dec-2024
DEFINITION  EHT1 Expression Cassette (F2620)
ACCESSION   BBa_P121107
VERSION     BBa_P121107.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     terminator      870..998
                     /label=BBa_B0015
     misc_feature    1007..1026
                     /label=LuxR/HSL
     promoter        1026..1031
                     /label=-35
     promoter        1048..1053
                     /label=-10
     misc_feature    1059..1059
                     /label=start
     misc_feature    1007..1061
                     /label=BBa_R0062
     promoter        1007..1061
                     /label=BBa_R0062
     misc_feature    1..1061
                     /label=BBa_F2620
     misc_feature    1074..1077
                     /label=conserved
     RBS             1070..1081
                     /label=BBa_B0034
     CDS             1088..2443
                     /label=BBa_C100007
     stem_loop       2463..2506
                     /label=stem_loop
     misc_feature    2452..2531
                     /label=BBa_B0010
     terminator      2452..2531
                     /label=BBa_B0010
     stem_loop       2547..2566
                     /label=T7 TE
     misc_feature    2573..2573
                     /label=stop
     polyA_site      2567..2580
                     /label=polya
     misc_feature    2540..2580
                     /label=BBa_B0012
     terminator      2540..2580
                     /label=BBa_B0012
     misc_feature    1070..2580
                     /label=BBa_P101107
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt
     1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa
     1081 atactagatg tcagaagttt ccaaatggcc agcaatcaac ccattccatt ggggatacaa
     1141 tggtacagtt tcgcatattg tcggtgaaaa tggttccatt aaactccatt taaaagacaa
     1201 caaggagcaa gttgattttg acgagttcgc taacaaatat gtcccaacgt tgaagaatgg
     1261 tgcccaattc aaattgagtc cttacttgtt cacaggtatt ttgcaaactt tgtacttagg
     1321 tgctgctgat ttctctaaga aatttcctgt attctacggc agggaaattg tcaaattctc
     1381 ggatggtgga gtttgcaccg ctgactggct catagattca tggaaaaagg attatgaatt
     1441 tgatcaaagt actacgagct ttgataaaaa aaaatttgat aaagacgaga aggcgacaca
     1501 tccagaagga tggcctcgtt tacaaccacg tacaaggtac ctgaaagata atgagttgga
     1561 agaactacgg gaggttgatc tacccctagt agttattcta catggtcttg ctggtggtag
     1621 tcatgagccg attataagat ctcttgctga aaacctgtct cgcagtggga gatttcaagt
     1681 ggtcgtccta aataccagag gttgtgcacg ttccaaaatt accaccagaa atttatttac
     1741 agcttatcac acaatggata ttcgcgagtt tttgcaaaga gaaaagcaaa gacatccaga
     1801 tagaaaacta tacgctgtgg gatgctcttt tggtgctacg atgctggcaa actatctggg
     1861 agaagagggc gataaatcac ctttatccgc agctgctact ttgtgcaatc cttgggatct
     1921 tctcctttca gcaattagga tgagccagga ttggtggtca agaactttat tttccaaaaa
     1981 tattgcgcaa ttcttaacaa gaaccgttca ggttaatatg ggtgaattag gagttccaaa
     2041 tggctctctc cccgatcatc ctcccacagt caagaatcca tctttctata tgttcacgcc
     2101 tgaaaatcta ataaaggcaa agagctttaa atcgacccgg gaatttgatg aagtgtacac
     2161 tgcgcctgct ttaggcttcc caaatgctat ggagtattat aaagcggcca gctcaataaa
     2221 cagagttgat acaattcggg ttcctaccct tgttatcaat tccagggatg atcctgttgt
     2281 cggcccagat caaccatact caatcgtgga aaagaatcct cgtattttgt attgtagaac
     2341 cgatttaggt ggtcatttag cttacctaga taaagacaac aactcgtggg ctaccaaggc
     2401 aattgcagaa tttttcacta agtttgatga attagtcgta tgatactaga gccaggcatc
     2461 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     2521 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//