LOCUS BBa_P121107 2580 bp DNA linear 25-Nov-2024 DEFINITION EHT1 Expression Cassette (F2620) ACCESSION BBa_P121107 VERSION BBa_P121107.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..82 /label=prefix misc_feature 254..254 /label=T misc_feature 572..572 /label=A CDS 81..830 /label=luxR misc_feature 81..836 /label=BBa_C0062 misc_feature 837..861 /label=Help:Barcodes CDS 81..836 /label=BBa_C0062 stem_loop 881..924 /label=stem_loop misc_feature 870..949 /label=BBa_B0010 terminator 870..949 /label=BBa_B0010 stem_loop 965..984 /label=T7 TE misc_feature 991..991 /label=stop polyA_site 985..998 /label=polya misc_feature 958..998 /label=BBa_B0012 terminator 958..998 /label=BBa_B0012 terminator 870..998 /label=BBa_B0015 misc_feature 1007..1026 /label=LuxR/HSL promoter 1026..1031 /label=-35 promoter 1048..1053 /label=-10 misc_feature 1059..1059 /label=start misc_feature 1007..1061 /label=BBa_R0062 promoter 1007..1061 /label=BBa_R0062 misc_feature 1..1061 /label=BBa_F2620 misc_feature 1074..1077 /label=conserved RBS 1070..1081 /label=BBa_B0034 CDS 1088..2443 /label=BBa_C100007 stem_loop 2463..2506 /label=stem_loop misc_feature 2452..2531 /label=BBa_B0010 terminator 2452..2531 /label=BBa_B0010 stem_loop 2547..2566 /label=T7 TE misc_feature 2573..2573 /label=stop polyA_site 2567..2580 /label=polya misc_feature 2540..2580 /label=BBa_B0012 terminator 2540..2580 /label=BBa_B0012 misc_feature 1070..2580 /label=BBa_P101107 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt 1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa 1081 atactagatg tcagaagttt ccaaatggcc agcaatcaac ccattccatt ggggatacaa 1141 tggtacagtt tcgcatattg tcggtgaaaa tggttccatt aaactccatt taaaagacaa 1201 caaggagcaa gttgattttg acgagttcgc taacaaatat gtcccaacgt tgaagaatgg 1261 tgcccaattc aaattgagtc cttacttgtt cacaggtatt ttgcaaactt tgtacttagg 1321 tgctgctgat ttctctaaga aatttcctgt attctacggc agggaaattg tcaaattctc 1381 ggatggtgga gtttgcaccg ctgactggct catagattca tggaaaaagg attatgaatt 1441 tgatcaaagt actacgagct ttgataaaaa aaaatttgat aaagacgaga aggcgacaca 1501 tccagaagga tggcctcgtt tacaaccacg tacaaggtac ctgaaagata atgagttgga 1561 agaactacgg gaggttgatc tacccctagt agttattcta catggtcttg ctggtggtag 1621 tcatgagccg attataagat ctcttgctga aaacctgtct cgcagtggga gatttcaagt 1681 ggtcgtccta aataccagag gttgtgcacg ttccaaaatt accaccagaa atttatttac 1741 agcttatcac acaatggata ttcgcgagtt tttgcaaaga gaaaagcaaa gacatccaga 1801 tagaaaacta tacgctgtgg gatgctcttt tggtgctacg atgctggcaa actatctggg 1861 agaagagggc gataaatcac ctttatccgc agctgctact ttgtgcaatc cttgggatct 1921 tctcctttca gcaattagga tgagccagga ttggtggtca agaactttat tttccaaaaa 1981 tattgcgcaa ttcttaacaa gaaccgttca ggttaatatg ggtgaattag gagttccaaa 2041 tggctctctc cccgatcatc ctcccacagt caagaatcca tctttctata tgttcacgcc 2101 tgaaaatcta ataaaggcaa agagctttaa atcgacccgg gaatttgatg aagtgtacac 2161 tgcgcctgct ttaggcttcc caaatgctat ggagtattat aaagcggcca gctcaataaa 2221 cagagttgat acaattcggg ttcctaccct tgttatcaat tccagggatg atcctgttgt 2281 cggcccagat caaccatact caatcgtgga aaagaatcct cgtattttgt attgtagaac 2341 cgatttaggt ggtcatttag cttacctaga taaagacaac aactcgtggg ctaccaaggc 2401 aattgcagaa tttttcacta agtttgatga attagtcgta tgatactaga gccaggcatc 2461 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 2521 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata //