LOCUS       BBa_P121108             2595 bp    DNA     linear       05-Nov-2024
DEFINITION  EEB1 Expression Cassette (F2620)
ACCESSION   BBa_P121108
VERSION     BBa_P121108.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     terminator      870..998
                     /label=BBa_B0015
     misc_feature    1007..1026
                     /label=LuxR/HSL
     promoter        1026..1031
                     /label=-35
     promoter        1048..1053
                     /label=-10
     misc_feature    1059..1059
                     /label=start
     misc_feature    1007..1061
                     /label=BBa_R0062
     promoter        1007..1061
                     /label=BBa_R0062
     misc_feature    1..1061
                     /label=BBa_F2620
     misc_feature    1074..1077
                     /label=conserved
     RBS             1070..1081
                     /label=BBa_B0034
     CDS             1088..2458
                     /label=BBa_C100008
     stem_loop       2478..2521
                     /label=stem_loop
     misc_feature    2467..2546
                     /label=BBa_B0010
     terminator      2467..2546
                     /label=BBa_B0010
     stem_loop       2562..2581
                     /label=T7 TE
     misc_feature    2588..2588
                     /label=stop
     polyA_site      2582..2595
                     /label=polya
     misc_feature    2555..2595
                     /label=BBa_B0012
     terminator      2555..2595
                     /label=BBa_B0012
     misc_feature    1070..2595
                     /label=BBa_P101108
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt
     1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa
     1081 atactagatg tttcgttctg gttattaccc taccgttact ccatcccatt ggggttataa
     1141 cggcaccgtt aaacatgtac tgggcgagaa aggcactaag tccctggcat tccgtgacag
     1201 caaacgtcag atcccgctgc atgaatttgt gactaaacac gtaccgaccc tgaaagacgg
     1261 cgccaacttc cgtctgaata gcctgctgtt caccggttat ctccagaccc tgtatttatc
     1321 cgcaggcgac ttcagcaaga aattccaggt gttctatggc cgtgagatca ttaaattctc
     1381 tgacggcggt gtgtgcaccg ctgattgggt tatgccggaa tgggaacaaa cttattccct
     1441 taatgcggag aaagcgtcgt tcaatgaaaa acagttcagc aacgacgaaa aggctactca
     1501 cccaaaaggc tggccgcgtc tgcacccgcg tactcgctac ctgtcctctg aggaactgga
     1561 aaaatgccac tctaaaggtt attcttaccc cctggtggtc gtactgcatg gtcttgccgg
     1621 tggctctcac gaaccgttaa tccgtgcact gtccgaagac ctgtccaagg tgggcgatgg
     1681 taaattccaa gtagttgtgc tgaatgcgcg tggctgctct cgctccaaag ttaccacccg
     1741 tcgtatcttt accgcgttgc acaccggcga cgtccgtgag ttcctgaacc atcagaaggc
     1801 tctgttcccg cagcgtaaaa tctacgctgt gggcacctcc ttcggtgcgg ctatgctgac
     1861 taactatctg ggtgaggaag gtgataactg tccgctgaac gctgcggtgg cactgtctaa
     1921 tccttgggat ttcgtacaca cctgggacaa actggcgcac gactggtggt ccaaccatat
     1981 tttctctcgt actctgaccc agtttctgac ccgcacggtt aaagtaaaca tgaacgaact
     2041 gcaagtaccg gagaacttcg aagtgagtca taagccgact gtcgaaaaac cggtcttcta
     2101 tacttataca cgtgaaaacc ttgaaaaagc tgaaaaattc accgacattc tcgagtttga
     2161 caacctgttc accgcgccgt ctatgggtct gccagatggt ctgacctact atcgtaaagc
     2221 ttctagtatc aaccgtctcc cgaacattaa aatcccaaca ctgattatca acgcaactga
     2281 tgacccggta actggagaaa acgtgatccc gtataagcag gcccgtgaaa acccatgcgt
     2341 tctgctgtgt gaaaccgatt taggtggtca cctggcctac ctggataacg aaagtaatag
     2401 ctggctgacc aaacaagctg cggagttcct gggtagcttt gacgaactgg ttctgtaata
     2461 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     2521 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     2581 ctttctgcgt ttata
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