LOCUS       BBa_P121112             2970 bp    DNA     linear       16-Feb-2025
DEFINITION  Myrcene Synthase Expression Cassette (F2620)
ACCESSION   BBa_P121112
VERSION     BBa_P121112.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     terminator      870..998
                     /label=BBa_B0015
     misc_feature    1007..1026
                     /label=LuxR/HSL
     promoter        1026..1031
                     /label=-35
     promoter        1048..1053
                     /label=-10
     misc_feature    1059..1059
                     /label=start
     misc_feature    1007..1061
                     /label=BBa_R0062
     promoter        1007..1061
                     /label=BBa_R0062
     misc_feature    1..1061
                     /label=BBa_F2620
     misc_feature    1074..1077
                     /label=conserved
     RBS             1070..1081
                     /label=BBa_B0034
     CDS             1088..2833
                     /label=BBa_C100012
     stem_loop       2853..2896
                     /label=stem_loop
     misc_feature    2842..2921
                     /label=BBa_B0010
     terminator      2842..2921
                     /label=BBa_B0010
     stem_loop       2937..2956
                     /label=T7 TE
     misc_feature    2963..2963
                     /label=stop
     polyA_site      2957..2970
                     /label=polya
     misc_feature    2930..2970
                     /label=BBa_B0012
     terminator      2930..2970
                     /label=BBa_B0012
     misc_feature    1070..2970
                     /label=BBa_P101112
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt
     1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa
     1081 atactagatg gctttttgta tttcttacgt aggtgctctg ctgccgtgtt ctctgagcac
     1141 ccgtactaaa tttgcgattt gccacaacac gtctaaactg caccgtgcgg cgtataaaac
     1201 gtcccgctgg aacatcccag gtgacgttgg cagcaccccg ccaccgtcca aactgcatca
     1261 agcactgtgc ctgaacgaac actctctgtc ctgcatggcg gaactgccaa tggattatga
     1321 gggtaaaatc aaagagactc gccacctgct gcacctgaaa ggtgaaaatg acccgatcga
     1381 atccctgatt tttgtggacg cgacgctgcg cctgggtgtc aaccatcact tccagaaaga
     1441 aatcgaagaa atcctgcgca aatcctacgc taccatgaag tccccgatca tctgcgaata
     1501 ccacaccctg cacgaagtaa gcctgttctt tcgcctgatg cgccagcacg gccgttacgt
     1561 aagcgcagac gtattcaata acttcaaggg cgaatccggt cgtttcaaag aagaactgaa
     1621 gcgcgatacc cgcggtctgg tggagctgta cgaagccgca cagctgtcct tcgaaggtga
     1681 acgcattctg gacgaagcgg aaaacttctc ccgtcagatc ctgcacggta acctggcggg
     1741 tatggaagac aacctgcgtc gctctgttgg caataaactg cgttatccgt ttcacacgtc
     1801 catcgctcgc ttcactggtc gtaactacga cgatgatctg ggtggcatgt acgaatgggg
     1861 taagaccctg cgtgagctgg cgctgatgga tctgcaagta gaacgttccg tttaccaaga
     1921 agaactgctg caagtgtcca aatggtggaa cgagctgggt ctgtacaaga aactgaacct
     1981 ggcgcgcaac cgtccgtttg aattttacac ctggtctatg gttatcctgg cagattatat
     2041 caacctgagc gaacagcgcg tggagctgac gaaaagcgtt gccttcatct atctgatcga
     2101 cgacatcttc gacgtgtacg gtaccctgga tgaactgatc atcttcaccg aagccgtgaa
     2161 taagtgggac tactccgcga ccgataccct gccagaaaac atgaagatgt gctgtatgac
     2221 gctgctggac actatcaatg gtacttccca gaaaatctat gagaaacacg gttataaccc
     2281 tatcgatagc ctgaagacca cctggaaaag cctgtgctct gctttcctgg tagaagctaa
     2341 atggtctgcg tccggcagcc tgccttctgc aaacgaatac ctggagaacg agaaagtgtc
     2401 ctctggcgtt tacgtggttc tggtccatct gttttgtctg atgggtctgg gcggtacttc
     2461 ccgcggtagc atcgaactga acgacaccca agaactgatg tcttccattg caatcatctt
     2521 tcgtctgtgg aatgacctgg gttccgccaa gaacgaacac cagaacggta aagatggtag
     2581 ctacctgaac tgttacaaga aagagcacat caatctgacc gctgcacagg cgcacgagca
     2641 cgcgctggaa ctggtggcta ttgagtggaa acgtctgaat aaagagtcct ttaacctgaa
     2701 ccacgactcc gtgagctcct tcaaacaagc agctctgaac ctggcacgca tggttccgct
     2761 gatgtactct tacgatcaca accagcgtgg tccagtactg gaggagtatg taaagtttat
     2821 gctgtctgac taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     2881 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     2941 caccttcggg tgggcctttc tgcgtttata
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