LOCUS       BBa_P121113             1953 bp    DNA     linear       22-Dec-2024
DEFINITION  PAD1 Expression Cassette (F2620)
ACCESSION   BBa_P121113
VERSION     BBa_P121113.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     terminator      870..998
                     /label=BBa_B0015
     misc_feature    1007..1026
                     /label=LuxR/HSL
     promoter        1026..1031
                     /label=-35
     promoter        1048..1053
                     /label=-10
     misc_feature    1059..1059
                     /label=start
     misc_feature    1007..1061
                     /label=BBa_R0062
     promoter        1007..1061
                     /label=BBa_R0062
     misc_feature    1..1061
                     /label=BBa_F2620
     misc_feature    1074..1077
                     /label=conserved
     RBS             1070..1081
                     /label=BBa_B0034
     CDS             1088..1816
                     /label=BBa_C100013
     stem_loop       1836..1879
                     /label=stem_loop
     misc_feature    1825..1904
                     /label=BBa_B0010
     terminator      1825..1904
                     /label=BBa_B0010
     stem_loop       1920..1939
                     /label=T7 TE
     misc_feature    1946..1946
                     /label=stop
     polyA_site      1940..1953
                     /label=polya
     misc_feature    1913..1953
                     /label=BBa_B0012
     terminator      1913..1953
                     /label=BBa_B0012
     misc_feature    1070..1953
                     /label=BBa_P101113
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt
     1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa
     1081 atactagatg ctcctatttc caagaagaac taatatagcc tttttcaaaa caacaggcat
     1141 ttttgctaat tttcctttgc taggtagaac cattacaact tcaccatctt tccttacaca
     1201 taaactgtca aaggaagtaa ccagggcatc aacttcgcct ccaagaccaa agagaattgt
     1261 tgtcgcaatt actggtgcga ctggtgttgc actgggaatc agacttctac aagtgctaaa
     1321 agagttgagc gtagaaaccc atttggtgat ttcaaaatgg ggtgcagcaa caatgaaata
     1381 tgaaacagat tgggaaccgc atgacgtggc ggccttggca accaagacat actctgttcg
     1441 tgatgtttct gcatgcattt cgtccggatc tttccagcat gatggtatga ttgttgtgcc
     1501 ctgttccatg aaatcactag ctgctattag aatcggtttt acagaggatt taattacaag
     1561 agctgccgat gtttcgatta aagagaatcg taagttacta ctggttactc gggaaacccc
     1621 tttatcttcc atccatcttg aaaacatgtt gtctttatgc agggcaggtg ttataatttt
     1681 tcctccggta cctgcgtttt atacaagacc caagagcctt catgacctat tagaacaaag
     1741 tgttggcagg atcctagact gctttggcat ccacgctgac acttttcctc gttgggaagg
     1801 aataaaaagc aagtaatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1861 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1921 gctcaccttc gggtgggcct ttctgcgttt ata
//