LOCUS BBa_P121115 3837 bp DNA linear 25-Nov-2024 DEFINITION LIS complete construct (F2620, B0034 optimized) ACCESSION BBa_P121115 VERSION BBa_P121115.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..82 /label=prefix misc_feature 254..254 /label=T misc_feature 572..572 /label=A CDS 81..830 /label=luxR misc_feature 81..836 /label=BBa_C0062 misc_feature 837..861 /label=Help:Barcodes CDS 81..836 /label=BBa_C0062 stem_loop 881..924 /label=stem_loop misc_feature 870..949 /label=BBa_B0010 terminator 870..949 /label=BBa_B0010 stem_loop 965..984 /label=T7 TE misc_feature 991..991 /label=stop polyA_site 985..998 /label=polya misc_feature 958..998 /label=BBa_B0012 terminator 958..998 /label=BBa_B0012 terminator 870..998 /label=BBa_B0015 misc_feature 1007..1026 /label=LuxR/HSL promoter 1026..1031 /label=-35 promoter 1048..1053 /label=-10 misc_feature 1059..1059 /label=start misc_feature 1007..1061 /label=BBa_R0062 promoter 1007..1061 /label=BBa_R0062 misc_feature 1..1061 /label=BBa_F2620 misc_feature 1074..1077 /label=conserved RBS 1070..1081 /label=BBa_B0034 CDS 1088..3700 /label=BBa_C100015 stem_loop 3720..3763 /label=stem_loop misc_feature 3709..3788 /label=BBa_B0010 terminator 3709..3788 /label=BBa_B0010 stem_loop 3804..3823 /label=T7 TE misc_feature 3830..3830 /label=stop polyA_site 3824..3837 /label=polya misc_feature 3797..3837 /label=BBa_B0012 terminator 3797..3837 /label=BBa_B0012 misc_feature 1070..3837 /label=BBa_P101115 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt 1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa 1081 atactagatg cagctgatta cgaacttcag cagcagctct agcgaactgc aattcctggt 1141 tgacaaagtt aaacgtgaat ctctgtcctc ctcctccagc aacactcaaa acctgtttct 1201 gtccacctct ccgtacgaca ctgcatggct ggcgctgatc ccgcaccctc accaccacca 1261 ccatcacggc cgtccaatgt ttgagaaatg cctgcaatgg atcctgcaca accagacccc 1321 gcaaggcttt tgggcagctg ctggcgacaa catctccgat actgacgacg atgtaaccct 1381 ggactgcctg ctgagcaccc tggcctgtct ggtcgcgctg aaacgctggc agctggctcc 1441 ggatatgatc cataaaggtc tggagtttgt gaatcgcaac accgagcgtc tggtgatgaa 1501 acagaaaccg tccgatgttc cgcgctggtt tactatcatg ttcccggcta tgctggagct 1561 ggctggcgct tctagcctgc gtgtagattt ctctgaaaac ctgaaccgta tcctggttga 1621 actgagccag aatcgtgacg atatcctgac tcgtgaagaa gtggatgaaa agaaacagta 1681 tagcccgctg ctgctgttcc tggaagcgct gccggctcag tcctacgaca atgatgtgct 1741 gaagcagatt attgataaga acctgagcaa cgacggttcc ctgctgcaat ctccgtctgc 1801 gactgcccgt gcttatatga tcactggtaa tacccgttgc ctgtcttacc tgcattctct 1861 gaccaactct tgcagcaacg gcggtgttcc atccttctac ccggtggatg acgacctgca 1921 tgatctggtt atggttaacc aactgacccg tagcggtctg actgaacacc tgattccaga 1981 aatcgatcac ctgctgctga aagtgcagaa aaactacaaa tacaagaaag cgagcccgaa 2041 aagcctgtac agcattgctg ctgagctgta ccgcgattct ctggccttct ggctgctgcg 2101 tgtcaacaac cactgggttt ctccgtctat cttttgctgg tttctggatg acgacgaaat 2161 tcgtgaccac attgaaacca actacgagga atttgctgcc gtgctgctga atgtttaccg 2221 tgcaacggac ctgatgtttt ccggtgaggt gcagctggtc gaagcccgtt ccttcgcgac 2281 taaaaacctg gaaaaaattc tggcaaccgg taacatccat aaaactaacg cggatatttc 2341 tagctctctg cacaaaatga ttgagcacga gctgcgcgtc ccgtggacgg cgcgtatgga 2401 ccatgtcgaa aaccgcattt ggatcgaaga gatcgcatcc agcgcactgt ggttcggtaa 2461 aagctcttac ctgcgcctga gctgcttcca taaaatgagc ctgcaacagc tggcggttaa 2521 aaactatact ctgcgtcagc tggtgtatcg tgacgaactg gcggaagttg aacgctggtc 2581 caaagagcgt ggcctgtgcg acatgggttt ctgccgtgag aaaaccggct actgttacta 2641 cgcttttgct gcaagcacct gtctgccgtg gagctctgac gtccgtctgg ttctgactaa 2701 agcagctgtt gtaattaccg tggcagacga cttcttcgat gtggagggta gcatggtgga 2761 cctggaaaaa ctgacggacg cagttcgtcg ttgggatgcg gaaggcctgg gctctcactc 2821 taagactatc ttcgaagctc tggatgacct ggtaaacgaa gtgcgcctga aatgtttcca 2881 acagaacggt caggacatca aaaacaacct gcaacaactg tggtacgaaa ccttccactc 2941 ttggctgatg gaagctaaat ggggcaaagg tctgacctcc aaaccgagcg ttgacgtgta 3001 cctgggtaac gctatgacgt ctattgcagc tcataccatg gtgctgactg caagctgtct 3061 gctgggtcct ggttttccag tgcaccagct gtggtcccag cgccgccacc aggatatcac 3121 ctccctgctg atggtactga ctcgcctgct gaacgacatc cagtcttacc tgaaagaaga 3181 agatgaaggt aagattaact acgtgtggat gtacatgatt gaaaacaacc aagccagcat 3241 cgacgattct gttcgccacg tgcagactat catcaacgtt aaaaagcagg aatttatcca 3301 acgtgttctg tccgatcagc actgcaacct gccgaaaagc ttcaagcaac tgcacttctc 3361 ttgcctgaaa gtgttcaaca tgtttttcaa ctcttctaac atcttcgata cggacaccga 3421 tctgctgctg gatatccacg aagcgtttgt gtctccacct caggtcccga aattcaaacc 3481 gcacatcaaa ccaccgcacc agctgccggc cactctgcaa ccgccacacc agccgcagca 3541 gattatggtt aacaagaaga aagtggaaat ggtttacaaa tcttatcacc atccgttcaa 3601 ggtatttacc ctgcaaaaga aacagtcctc tggtcacggt accatgaatc ctcgtgcgtc 3661 tatcctggct ggtccaaaca tcaaactgtg tttcagctaa tactagagcc aggcatcaaa 3721 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 3781 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata //