LOCUS       BBa_P121116             2964 bp    DNA     linear       26-Dec-2024
DEFINITION  Oe23 complete construct (F2620, B0034 optimized)
ACCESSION   BBa_P121116
VERSION     BBa_P121116.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     terminator      870..998
                     /label=BBa_B0015
     misc_feature    1007..1026
                     /label=LuxR/HSL
     promoter        1026..1031
                     /label=-35
     promoter        1048..1053
                     /label=-10
     misc_feature    1059..1059
                     /label=start
     misc_feature    1007..1061
                     /label=BBa_R0062
     promoter        1007..1061
                     /label=BBa_R0062
     misc_feature    1..1061
                     /label=BBa_F2620
     misc_feature    1074..1077
                     /label=conserved
     RBS             1070..1081
                     /label=BBa_B0034
     CDS             1088..2827
                     /label=BBa_C100016
     stem_loop       2847..2890
                     /label=stem_loop
     misc_feature    2836..2915
                     /label=BBa_B0010
     terminator      2836..2915
                     /label=BBa_B0010
     stem_loop       2931..2950
                     /label=T7 TE
     misc_feature    2957..2957
                     /label=stop
     polyA_site      2951..2964
                     /label=polya
     misc_feature    2924..2964
                     /label=BBa_B0012
     terminator      2924..2964
                     /label=BBa_B0012
     misc_feature    1070..2964
                     /label=BBa_P101116
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt
     1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa
     1081 atactagatg gctttctgca tcagctatct gggtgctgtt ctgccgttca gcttgagccc
     1141 acgtactaaa ttcgccatct tccataacac ctccaagcat gccgcttata aaacttgccg
     1201 ctggaacatc ccacgtgatg tgggtagcac cccgccaccg tccaagctgc atcaggctct
     1261 gtgcctgaat gcgcactcta ctagctgcat ggctgaactg ccgatggatt acgagggcaa
     1321 gattcagggc acccgtcacc tgctgcacct gaaagacgaa aatgacccaa tcgaatccct
     1381 gatctttgtc gacgccaccc agcgtctggg cgtgaaccac cacttccaga aagagatcga
     1441 agaaatcctg cgtaaatctt acgcgacgat gaaatccccg tccatctgca aataccacac
     1501 cctgcacgat gtgtccctgt tcttctgtct gatgcgtcag catggtcgct atgtaagcgc
     1561 ggacgtattc aataacttca aaggcgaaag cggccgtttc aaagaggaac tgaagcgtga
     1621 cactcgtggc ctggttgaac tgtacgaggc ggcacagctg tcttttgagg gcgaacgcat
     1681 cctggatgaa gcggaaaact tctctcgtca gatcctgcac ggcaacctgg caagcatgga
     1741 ggacaatctg cgccgctctg tgggcaacaa gctgcgttac ccattccaca aatccattgc
     1801 gcgttttacg ggtatcaact acgatgacga cctgggcggt atgtacgaat ggggtaagac
     1861 cctgcgtgaa ctggcgctga tggacctgca agtggaacgt tctgtttacc aggaagagct
     1921 gctgcaagtg tctaaatggt ggaatgaact gggcctgtat aagaaactga ccctggctcg
     1981 caaccgtccg ttcgaatttt acatgtggtc tatggtaatc ctgactgact acattaacct
     2041 gtccgaacaa cgtgtagaac tgacgaagtc tgttgcattc atctacctga ttgacgatat
     2101 ctttgatgtg tacggtactc tggacgaact gatcatcttc actgaagcgg ttaacaaatg
     2161 ggactattct gcaacggata ctctgccaga caacatgaaa atgtgctata tgactctgct
     2221 ggataccatc aacggtacta gccagaaaat ctacgaaaaa tacggccaca atccaattga
     2281 ctctctgaaa actacttgga agagcctgtg tagcgccttc ctggtagaag cgaaatggtc
     2341 tgcgtccggc agcctgccgt ctgcaaacga atatctggaa aacgaaaaag tctcctccgg
     2401 tgtgtatgtt gtgctgatcc atctgttctt tctgatgggc ctgggcggta ccaaccgcgg
     2461 ctctatcgaa ctgaatgaca cccgcgaact gatgtcctct attgcgatta tcgtgcgtat
     2521 ctggaacgat ctgggttgtg cgaaaaacga acaccagaac ggtaaagacg gttcttacct
     2581 ggactgctac aagaaggaac acatcaacct gactgcggcg caggttcacg aacatgcgct
     2641 ggaactggtg gccatcgaat ggaaacgcct gaacaaggaa tctttcaacc tgaaccacga
     2701 tagcgtatcc tcttttaagc aggcagcgct gaacttcgcc cgtatggtac cgctgatgta
     2761 tagctatgac aacaaccgtc gtggtccggt cctggaagaa tatgtcaaat ttatgctgtc
     2821 tgattaatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     2881 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     2941 cgggtgggcc tttctgcgtt tata
//