##gff-version 3
##sequence-region BBa_P20050 1 1547
BBa_P20050	.	engineered_region	1070	1547	.	+	0	ID=BBa_Q20050;Name=BBa_Q20050
BBa_P20050	.	ribosome_entry_site	1070	1084	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_Q20050
BBa_P20050	.	engineered_region	1070	1084	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_P20050	.	ribosome_entry_site	1070	1084	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_P20050	.	ribosome_entry_site	1077	1081	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_P20050	.	terminator	1366	1445	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q20050
BBa_P20050	.	stem_loop	1377	1420	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_P20050	.	engineered_region	1366	1445	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_P20050	.	terminator	1454	1494	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q20050
BBa_P20050	.	engineered_region	1454	1494	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_P20050	.	stop_codon	1487	1487	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_P20050	.	polyA_site	1481	1494	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_P20050	.	stem_loop	1461	1480	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_P20050	.	CDS	1091	1357	.	+	0	ID=BBa_C2005;Name=BBa_C2005;Parent=BBa_Q20050
BBa_P20050	.	non_covalent_binding_site	1094	1180	.	+	0	ID=annotation1903902;Name=finger 2 of Zif268;Parent=BBa_C2005
BBa_P20050	.	stop_codon	1352	1357	.	+	0	ID=annotation1903907;Name=double stop codon;Parent=BBa_C2005
BBa_P20050	.	non_covalent_binding_site	1181	1240	.	+	0	ID=annotation1903903;Name=finger 3 of Zif268;Parent=BBa_C2005
BBa_P20050	.	start_codon	1091	1093	.	+	0	ID=annotation1903904;Name=start codon;Parent=BBa_C2005
BBa_P20050	.	CDS	1091	1357	.	+	0	ID=annotation1903901;Name=Zif268.GCN4;Parent=BBa_C2005
BBa_P20050	.	sequence_feature	1241	1255	.	+	0	ID=annotation1903905;Name=domain linker;Parent=BBa_C2005
BBa_P20050	.	non_covalent_binding_site	1256	1351	.	+	0	ID=annotation1903906;Name=GCN4 leucine zipper homodimerization domain;Parent=BBa_C2005
BBa_P20050	.	promoter	1503	1547	.	+	0	ID=BBa_R2000;Name=BBa_R2000;Parent=BBa_Q20050
BBa_P20050	.	start_codon	1547	1547	.	+	0	ID=annotation318102;Name=(+1);Parent=BBa_R2000
BBa_P20050	.	promoter	1535	1540	.	+	0	ID=annotation318098;Name=-10;Parent=BBa_R2000
BBa_P20050	.	non_covalent_binding_site	1522	1535	.	+	0	ID=annotation318099;Name=Zif23;Parent=BBa_R2000
BBa_P20050	.	sequence_feature	1503	1511	.	+	0	ID=annotation318100;Name=consensus;Parent=BBa_R2000
BBa_P20050	.	promoter	1512	1517	.	+	0	ID=annotation318094;Name=-35;Parent=BBa_R2000
BBa_P20050	.	engineered_region	1	1061	.	+	0	ID=BBa_F2620;Name=BBa_F2620
BBa_P20050	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2620
BBa_P20050	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_P20050	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_F2620
BBa_P20050	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_P20050	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_P20050	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_P20050	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_P20050	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_P20050	.	promoter	1007	1061	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2620
BBa_P20050	.	promoter	1026	1031	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_P20050	.	engineered_region	1007	1061	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_P20050	.	start_codon	1059	1059	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_P20050	.	promoter	1048	1053	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_P20050	.	non_covalent_binding_site	1007	1026	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_P20050	.	terminator	870	998	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_F2620
BBa_P20050	.	terminator	870	949	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_P20050	.	stem_loop	881	924	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_P20050	.	engineered_region	870	949	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_P20050	.	terminator	958	998	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_P20050	.	engineered_region	958	998	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_P20050	.	stop_codon	991	991	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_P20050	.	polyA_site	985	998	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_P20050	.	stem_loop	965	984	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_P20050	.	CDS	81	836	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2620
BBa_P20050	.	point_mutation	572	572	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_P20050	.	engineered_region	81	836	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_P20050	.	engineered_region	81	82	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_P20050	.	CDS	81	830	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_P20050	.	point_mutation	254	254	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_P20050	.	engineered_tag	837	861	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
>BBa_P20050
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaat
caatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtta
aatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatga
tcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaa
tctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatg
gcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacat
accattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaacc
aaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtg
agcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagc
aattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtt
tacgcaagaaaatggtttgttatagtcgaataaatactagagattaaagaggagaaatactagatgaaaccgttccagt
gccgtatctgcatgcgtaacttctcccgttccgaccacctgaccacccacatccgtacccacaccggtgaaaaaccgtt
cgcttgcgacatctgcggtcgtaaattcgctcgttccgacgaacgtaaacgtcaccgtaaattgcagcacatgaaacag
ctggaagacaaagttgaagaactgctgtccaaaaactaccacctggaaaacgaagttgctcgtctgaaaaaactggttg
gtgaacgttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatct
gttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactaga
gagtttattcttgacatggtcccacgcgcgtgggatactacgtcag