LOCUS BBa_P20070 2648 bp DNA linear 22-Nov-2024 DEFINITION 3OC6HSL->Inverter->PoPS ACCESSION BBa_P20070 VERSION BBa_P20070.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..82 /label=prefix misc_feature 254..254 /label=T misc_feature 572..572 /label=A CDS 81..830 /label=luxR misc_feature 81..836 /label=BBa_C0062 misc_feature 837..861 /label=Help:Barcodes CDS 81..836 /label=BBa_C0062 stem_loop 881..924 /label=stem_loop misc_feature 870..949 /label=BBa_B0010 terminator 870..949 /label=BBa_B0010 stem_loop 965..984 /label=T7 TE misc_feature 991..991 /label=stop polyA_site 985..998 /label=polya misc_feature 958..998 /label=BBa_B0012 terminator 958..998 /label=BBa_B0012 terminator 870..998 /label=BBa_B0015 misc_feature 1007..1026 /label=LuxR/HSL promoter 1026..1031 /label=-35 promoter 1048..1053 /label=-10 misc_feature 1059..1059 /label=start misc_feature 1007..1061 /label=BBa_R0062 promoter 1007..1061 /label=BBa_R0062 misc_feature 1..1061 /label=BBa_F2620 RBS 1070..1084 /label=RBS-1\Strong RBS 1077..1081 /label=RBS misc_feature 1070..1084 /label=BBa_B0030 RBS 1070..1084 /label=BBa_B0030 CDS 1091..2458 /label=BBa_C2007 stem_loop 2478..2521 /label=stem_loop misc_feature 2467..2546 /label=BBa_B0010 terminator 2467..2546 /label=BBa_B0010 stem_loop 2562..2581 /label=T7 TE misc_feature 2588..2588 /label=stop polyA_site 2582..2595 /label=polya misc_feature 2555..2595 /label=BBa_B0012 terminator 2555..2595 /label=BBa_B0012 promoter 2613..2618 /label=-35 promoter 2636..2641 /label=-10 misc_feature 2623..2636 /label=Zif23 misc_feature 2604..2612 /label=consensus misc_feature 2648..2648 /label=(+1) promoter 2604..2648 /label=BBa_R2000 misc_feature 1070..2648 /label=BBa_Q20070 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt 1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga ttaaagagga 1081 gaaatactag atgaaaccgt tccagtgccg tatctgcatg cgtaacttct cccgttccga 1141 ccacctgacc acccacatcc gtacccacac cggtgaaaaa ccgttcgctt gcgacatctg 1201 cggtcgtaaa ttcgctcgtt ccgacgaacg taaacgtcac cgtaaattgc agcacatgaa 1261 acagctggaa gacaaagttg aagaactgct gtccaaaaac taccacctgg aaaacgaagt 1321 tgctcgtctg aaaaaactgg ttggtgaacg tatgaaaatc gaagaaggta aactggtaat 1381 ctggattaac ggcgataaag gctataacgg tctcgctgaa gtcggtaaga aattcgagaa 1441 agataccgga attaaagtca ccgttgagca tccggataaa ctggaagaga aattcccaca 1501 ggttgcggca actggcgatg gccctgacat tatcttctgg gcacacgacc gctttggtgg 1561 ctacgctcaa tctggcctgt tggctgaaat caccccggac aaagcgttcc aggacaagct 1621 gtatccgttt acctgggatg ccgtacgtta caacggcaag ctgattgctt acccgatcgc 1681 tgttgaagcg ttatcgctga tttataacaa agatctgctg ccgaacccgc caaaaacctg 1741 ggaagagatc ccggcgctgg ataaagaact gaaagcgaaa ggtaagagcg cgctgatgtt 1801 caacctgcaa gaaccgtact tcacctggcc gctgattgct gctgacgggg gttatgcgtt 1861 caagtatgaa aacggcaagt acgacattaa agacgtgggc gtggataacg ctggcgcgaa 1921 agcgggtctg accttcctgg ttgacctgat taaaaacaaa cacatgaatg cagacaccga 1981 ttactccatc gcagaagctg cctttaataa aggcgaaaca gcgatgacca tcaacggccc 2041 gtgggcatgg tccaacatcg acaccagcaa agtgaattat ggtgtaacgg tactgccgac 2101 cttcaagggt caaccatcca aaccgttcgt tggcgtgctg agcgcaggta ttaacgccgc 2161 cagtccgaac aaagagctgg caaaagagtt cctcgaaaac tatctgctga ctgatgaagg 2221 tctggaagcg gttaataaag acaaaccgct gggtgccgta gcgctgaagt cttacgagga 2281 agagttggcg aaagatccac gtattgccgc cactatggaa aacgcccaga aaggtgaaat 2341 catgccgaac atcccgcaga tgtccgcttt ctggtatgcc gtgcgtactg cggtgatcaa 2401 cgccgccagc ggtcgtcaga ctgtcgatga agccctgaaa gacgcgcaga cttaataata 2461 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 2521 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 2581 ctttctgcgt ttatatacta gagagtttat tcttgacatg gtcccacgcg cgtgggatac 2641 tacgtcag //