LOCUS       BBa_P2105               1494 bp    DNA     linear       09-May-2024
DEFINITION  Zif268-GCN4 protein generator
ACCESSION   BBa_P2105
VERSION     BBa_P2105.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     terminator      870..998
                     /label=BBa_B0015
     misc_feature    1007..1026
                     /label=LuxR/HSL
     promoter        1026..1031
                     /label=-35
     promoter        1048..1053
                     /label=-10
     misc_feature    1059..1059
                     /label=start
     misc_feature    1007..1061
                     /label=BBa_R0062
     promoter        1007..1061
                     /label=BBa_R0062
     misc_feature    1..1061
                     /label=BBa_F2620
     RBS             1070..1084
                     /label=RBS-1\Strong
     RBS             1077..1081
                     /label=RBS
     misc_feature    1070..1084
                     /label=BBa_B0030
     RBS             1070..1084
                     /label=BBa_B0030
     CDS             1091..1357
                     /label=Zif268.GCN4
     misc_feature    1094..1180
                     /label=finger 2 of Zif268
     misc_feature    1181..1240
                     /label=finger 3 of Zif268
     misc_feature    1091..1093
                     /label=start codon
     misc_feature    1241..1255
                     /label=domain linker
     misc_feature    1256..1351
                     /label=GCN4 leucine zipper homodimerization domain
     misc_feature    1352..1357
                     /label=double stop codon
     CDS             1091..1357
                     /label=BBa_C2005
     stem_loop       1377..1420
                     /label=stem_loop
     misc_feature    1366..1445
                     /label=BBa_B0010
     terminator      1366..1445
                     /label=BBa_B0010
     stem_loop       1461..1480
                     /label=T7 TE
     misc_feature    1487..1487
                     /label=stop
     polyA_site      1481..1494
                     /label=polya
     misc_feature    1454..1494
                     /label=BBa_B0012
     terminator      1454..1494
                     /label=BBa_B0012
     misc_feature    1070..1494
                     /label=BBa_P2005
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt
     1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga ttaaagagga
     1081 gaaatactag atgaaaccgt tccagtgccg tatctgcatg cgtaacttct cccgttccga
     1141 ccacctgacc acccacatcc gtacccacac cggtgaaaaa ccgttcgctt gcgacatctg
     1201 cggtcgtaaa ttcgctcgtt ccgacgaacg taaacgtcac cgtaaattgc agcacatgaa
     1261 acagctggaa gacaaagttg aagaactgct gtccaaaaac taccacctgg aaaacgaagt
     1321 tgctcgtctg aaaaaactgg ttggtgaacg ttaataatac tagagccagg catcaaataa
     1381 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     1441 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//