LOCUS       BBa_P2107               2595 bp    DNA     linear       09-May-2024
DEFINITION  Zif268-GCN4.MalE protein generator
ACCESSION   BBa_P2107
VERSION     BBa_P2107.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     terminator      870..998
                     /label=BBa_B0015
     misc_feature    1007..1026
                     /label=LuxR/HSL
     promoter        1026..1031
                     /label=-35
     promoter        1048..1053
                     /label=-10
     misc_feature    1059..1059
                     /label=start
     misc_feature    1007..1061
                     /label=BBa_R0062
     promoter        1007..1061
                     /label=BBa_R0062
     misc_feature    1..1061
                     /label=BBa_F2620
     RBS             1070..1084
                     /label=RBS-1\Strong
     RBS             1077..1081
                     /label=RBS
     misc_feature    1070..1084
                     /label=BBa_B0030
     RBS             1070..1084
                     /label=BBa_B0030
     CDS             1091..2458
                     /label=BBa_C2007
     stem_loop       2478..2521
                     /label=stem_loop
     misc_feature    2467..2546
                     /label=BBa_B0010
     terminator      2467..2546
                     /label=BBa_B0010
     stem_loop       2562..2581
                     /label=T7 TE
     misc_feature    2588..2588
                     /label=stop
     polyA_site      2582..2595
                     /label=polya
     misc_feature    2555..2595
                     /label=BBa_B0012
     terminator      2555..2595
                     /label=BBa_B0012
     misc_feature    1070..2595
                     /label=BBa_P2007
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt
     1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga ttaaagagga
     1081 gaaatactag atgaaaccgt tccagtgccg tatctgcatg cgtaacttct cccgttccga
     1141 ccacctgacc acccacatcc gtacccacac cggtgaaaaa ccgttcgctt gcgacatctg
     1201 cggtcgtaaa ttcgctcgtt ccgacgaacg taaacgtcac cgtaaattgc agcacatgaa
     1261 acagctggaa gacaaagttg aagaactgct gtccaaaaac taccacctgg aaaacgaagt
     1321 tgctcgtctg aaaaaactgg ttggtgaacg tatgaaaatc gaagaaggta aactggtaat
     1381 ctggattaac ggcgataaag gctataacgg tctcgctgaa gtcggtaaga aattcgagaa
     1441 agataccgga attaaagtca ccgttgagca tccggataaa ctggaagaga aattcccaca
     1501 ggttgcggca actggcgatg gccctgacat tatcttctgg gcacacgacc gctttggtgg
     1561 ctacgctcaa tctggcctgt tggctgaaat caccccggac aaagcgttcc aggacaagct
     1621 gtatccgttt acctgggatg ccgtacgtta caacggcaag ctgattgctt acccgatcgc
     1681 tgttgaagcg ttatcgctga tttataacaa agatctgctg ccgaacccgc caaaaacctg
     1741 ggaagagatc ccggcgctgg ataaagaact gaaagcgaaa ggtaagagcg cgctgatgtt
     1801 caacctgcaa gaaccgtact tcacctggcc gctgattgct gctgacgggg gttatgcgtt
     1861 caagtatgaa aacggcaagt acgacattaa agacgtgggc gtggataacg ctggcgcgaa
     1921 agcgggtctg accttcctgg ttgacctgat taaaaacaaa cacatgaatg cagacaccga
     1981 ttactccatc gcagaagctg cctttaataa aggcgaaaca gcgatgacca tcaacggccc
     2041 gtgggcatgg tccaacatcg acaccagcaa agtgaattat ggtgtaacgg tactgccgac
     2101 cttcaagggt caaccatcca aaccgttcgt tggcgtgctg agcgcaggta ttaacgccgc
     2161 cagtccgaac aaagagctgg caaaagagtt cctcgaaaac tatctgctga ctgatgaagg
     2221 tctggaagcg gttaataaag acaaaccgct gggtgccgta gcgctgaagt cttacgagga
     2281 agagttggcg aaagatccac gtattgccgc cactatggaa aacgcccaga aaggtgaaat
     2341 catgccgaac atcccgcaga tgtccgcttt ctggtatgcc gtgcgtactg cggtgatcaa
     2401 cgccgccagc ggtcgtcaga ctgtcgatga agccctgaaa gacgcgcaga cttaataata
     2461 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     2521 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     2581 ctttctgcgt ttata
//