LOCUS       BBa_Q00400               905 bp    DNA     linear       18-May-2024
DEFINITION  Tet
ACCESSION   BBa_Q00400
VERSION     BBa_Q00400.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=RBS-1\Strong
     RBS             8..12
                     /label=RBS
     misc_feature    1..15
                     /label=BBa_B0030
     RBS             1..15
                     /label=BBa_B0030
     CDS             25..641
                     /label=tetR
     misc_feature    642..675
                     /label=SsrA
     misc_feature    682..706
                     /label=Help:Barcodes
     CDS             22..681
                     /label=BBa_C0040
     stem_loop       726..769
                     /label=stem_loop
     misc_feature    715..794
                     /label=BBa_B0010
     terminator      715..794
                     /label=BBa_B0010
     stem_loop       810..829
                     /label=T7 TE
     misc_feature    836..836
                     /label=stop
     polyA_site      830..843
                     /label=polya
     misc_feature    803..843
                     /label=BBa_B0012
     terminator      803..843
                     /label=BBa_B0012
     misc_feature    852..870
                     /label=TetR 1
     misc_feature    852..905
                     /label=BBa_R0040
     promoter        871..876
                     /label=-35
     misc_feature    877..895
                     /label=TetR 2
     promoter        894..899
                     /label=-10
     promoter        852..905
                     /label=BBa_R0040
ORIGIN
        1 attaaagagg agaaatacta gatgtccaga ttagataaaa gtaaagtgat taacagcgca
       61 ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag
      121 ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac
      181 gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa
      241 agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt actaagtcat
      301 cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc
      361 gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca
      421 ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc
      481 gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct
      541 atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct tgaattgatc
      601 atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac
      661 tacgctttag tagcttaata acactgatag tgctagtgta gatcactact agagccaggc
      721 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      781 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      841 atatactaga gtccctatca gtgatagaga ttgacatccc tatcagtgat agagatactg
      901 agcac
//