##gff-version 3
##sequence-region BBa_Q00400 1 905
BBa_Q00400	.	terminator	715	794	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_Q00400	.	stem_loop	726	769	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_Q00400	.	engineered_region	715	794	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_Q00400	.	promoter	852	905	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_Q00400	.	engineered_region	852	905	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_Q00400	.	non_covalent_binding_site	852	870	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_Q00400	.	non_covalent_binding_site	877	895	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_Q00400	.	promoter	894	899	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_Q00400	.	promoter	871	876	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_Q00400	.	CDS	22	681	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_Q00400	.	tag	642	675	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_Q00400	.	engineered_tag	682	706	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_Q00400	.	CDS	25	641	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_Q00400	.	ribosome_entry_site	1	15	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_Q00400	.	ribosome_entry_site	8	12	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_Q00400	.	ribosome_entry_site	1	15	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_Q00400	.	engineered_region	1	15	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_Q00400	.	terminator	803	843	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_Q00400	.	polyA_site	830	843	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_Q00400	.	engineered_region	803	843	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_Q00400	.	stem_loop	810	829	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_Q00400	.	stop_codon	836	836	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
>BBa_Q00400
attaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgagg
tcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaa
aaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggg
gaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaag
tacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggttt
ttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcat
caagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttg
atcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtga
aagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactacta
gagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgc
tctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagag
attgacatccctatcagtgatagagatactgagcac