##gff-version 3
##sequence-region BBa_Q02400 1 903
BBa_Q02400	.	ribosome_entry_site	1	13	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_Q02400	.	engineered_region	1	13	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_Q02400	.	ribosome_entry_site	1	13	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_Q02400	.	ribosome_entry_site	7	10	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_Q02400	.	terminator	801	841	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_Q02400	.	polyA_site	828	841	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_Q02400	.	engineered_region	801	841	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_Q02400	.	stem_loop	808	827	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_Q02400	.	stop_codon	834	834	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_Q02400	.	CDS	20	679	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_Q02400	.	tag	640	673	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_Q02400	.	engineered_tag	680	704	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_Q02400	.	CDS	23	639	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_Q02400	.	promoter	850	903	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_Q02400	.	engineered_region	850	903	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_Q02400	.	non_covalent_binding_site	850	868	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_Q02400	.	non_covalent_binding_site	875	893	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_Q02400	.	promoter	892	897	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_Q02400	.	promoter	869	874	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_Q02400	.	terminator	713	792	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_Q02400	.	stem_loop	724	767	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_Q02400	.	engineered_region	713	792	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_Q02400
tcacacaggaaagtactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtc
ggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaa
ataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaagggga
aagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagta
catttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggttttt
cactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatca
agtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgat
caccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaa
gtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagat
tgacatccctatcagtgatagagatactgagcac