LOCUS       BBa_Q02740               689 bp    DNA     linear       22-Nov-2024
DEFINITION  PenI quad part inverter
ACCESSION   BBa_Q02740
VERSION     BBa_Q02740.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     CDS             20..406
                     /label=penI
     misc_feature    404..436
                     /label=LVA
     misc_feature    443..467
                     /label=Help:Barcodes
     CDS             20..442
                     /label=BBa_C0074
     stem_loop       487..530
                     /label=stem_loop
     misc_feature    476..555
                     /label=BBa_B0010
     terminator      476..555
                     /label=BBa_B0010
     stem_loop       571..590
                     /label=T7 TE
     misc_feature    597..597
                     /label=stop
     polyA_site      591..604
                     /label=polya
     misc_feature    564..604
                     /label=BBa_B0012
     terminator      564..604
                     /label=BBa_B0012
     misc_feature    613..689
                     /label=PenI
     promoter        635..640
                     /label=-35
     promoter        658..663
                     /label=-10
     misc_feature    643..665
                     /label=dimer left half
     misc_feature    667..688
                     /label=dimer right half
     promoter        613..689
                     /label=BBa_R0074
ORIGIN
        1 tcacacagga aagtactaga tgaaaaaaat accacagatt tctgatgcgg aactcgaagt
       61 catgaaagtg atttggaagc attcttccat taatactaat gaggtaatca aagagctcag
      121 taaaacttca acgtggagcc caaaaactat tcagactatg ctgctgcgcc ttatcaaaaa
      181 aggcgctctc aaccaccata aagaaggtcg ggttttcgtt tacacgccta atatagacga
      241 atcagattat atagaggtta agtcacactc atttctcaac cggttttaca atggtacatt
      301 aaattccatg gtactcaact ttttggagaa tgatcaactg tcgggagaag aaatcaatga
      361 attgtatcag atactcgaag aacataagaa ccgtaagaag gaagctgcaa acgacgaaaa
      421 ctacgcttta gtagcttaat aactctgata gtgctagtgt agatctctac tagagccagg
      481 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
      541 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
      601 tatatactag agtcatttcc aaccgaaaaa aggcttgcat ttaaatctta catatgtaat
      661 actttcaaag actacatttg taagatttg
//