LOCUS       BBa_Q03122              1515 bp    DNA     linear       15-Dec-2024
DEFINITION  QPI (B0033.C0012.B0015.R0010)
ACCESSION   BBa_Q03122
VERSION     BBa_Q03122.1  
FEATURES             Location/Qualifiers
     RBS             1..11
                     /label=RBS-4\Weaker
     RBS             7..10
                     /label=RBS
     misc_feature    1..11
                     /label=BBa_B0033
     RBS             1..11
                     /label=BBa_B0033
     misc_feature    1107..1145
                     /label=LVA
     CDS             18..1145
                     /label=lacI-LVA
     misc_feature    18..1145
                     /label=BBa_C0012
     misc_feature    1146..1170
                     /label=Help:Barcodes
     CDS             18..1145
                     /label=BBa_C0012
     stem_loop       1190..1233
                     /label=stem_loop
     misc_feature    1179..1258
                     /label=BBa_B0010
     terminator      1179..1258
                     /label=BBa_B0010
     stem_loop       1274..1293
                     /label=T7 TE
     misc_feature    1300..1300
                     /label=stop
     polyA_site      1294..1307
                     /label=polya
     misc_feature    1267..1307
                     /label=BBa_B0012
     terminator      1267..1307
                     /label=BBa_B0012
     CDS             1316..1403
                     /label=end of LacI coding region (inactive)
     misc_feature    1316..1515
                     /label=BBa_R0010
     misc_feature    1404..1441
                     /label=CAP binding site
     promoter        1452..1457
                     /label=-35
     promoter        1476..1481
                     /label=-10
     misc_feature    1481..1515
                     /label=LacI binding site
     misc_feature    1488..1488
                     /label=start
     promoter        1316..1515
                     /label=BBa_R0010
ORIGIN
        1 tcacacagga ctactagatg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt
       61 atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg
      121 cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg
      181 tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg
      241 ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg
      301 ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc
      361 acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg
      421 atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg
      481 accagacacc catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg
      541 agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg
      601 tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc
      661 cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa
      721 tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg
      781 gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg
      841 gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc atcaaacagg
      901 attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg
      961 cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcgc
     1021 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     1081 aggtttcccg actggaaagc gggcaggctg caaacgacga aaactacgct ttagtagctt
     1141 aataactctg atagtgctag tgtagatctc tactagagcc aggcatcaaa taaaacgaaa
     1201 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     1261 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagcaata
     1321 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     1381 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     1441 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     1501 taacaatttc acaca
//