LOCUS       BBa_Q03400               901 bp    DNA     linear       22-Nov-2024
DEFINITION  QPI (tetR)
ACCESSION   BBa_Q03400
VERSION     BBa_Q03400.1  
FEATURES             Location/Qualifiers
     RBS             1..11
                     /label=RBS-4\Weaker
     RBS             7..10
                     /label=RBS
     misc_feature    1..11
                     /label=BBa_B0033
     RBS             1..11
                     /label=BBa_B0033
     CDS             21..637
                     /label=tetR
     misc_feature    638..671
                     /label=SsrA
     misc_feature    678..702
                     /label=Help:Barcodes
     CDS             18..677
                     /label=BBa_C0040
     stem_loop       722..765
                     /label=stem_loop
     misc_feature    711..790
                     /label=BBa_B0010
     terminator      711..790
                     /label=BBa_B0010
     stem_loop       806..825
                     /label=T7 TE
     misc_feature    832..832
                     /label=stop
     polyA_site      826..839
                     /label=polya
     misc_feature    799..839
                     /label=BBa_B0012
     terminator      799..839
                     /label=BBa_B0012
     misc_feature    848..866
                     /label=TetR 1
     misc_feature    848..901
                     /label=BBa_R0040
     promoter        867..872
                     /label=-35
     misc_feature    873..891
                     /label=TetR 2
     promoter        890..895
                     /label=-10
     promoter        848..901
                     /label=BBa_R0040
ORIGIN
        1 tcacacagga ctactagatg tccagattag ataaaagtaa agtgattaac agcgcattag
       61 agctgcttaa tgaggtcgga atcgaaggtt taacaacccg taaactcgcc cagaagctag
      121 gtgtagagca gcctacattg tattggcatg taaaaaataa gcgggctttg ctcgacgcct
      181 tagccattga gatgttagat aggcaccata ctcacttttg ccctttagaa ggggaaagct
      241 ggcaagattt tttacgtaat aacgctaaaa gttttagatg tgctttacta agtcatcgcg
      301 atggagcaaa agtacattta ggtacacggc ctacagaaaa acagtatgaa actctcgaaa
      361 atcaattagc ctttttatgc caacaaggtt tttcactaga gaatgcatta tatgcactca
      421 gcgctgtggg gcattttact ttaggttgcg tattggaaga tcaagagcat caagtcgcta
      481 aagaagaaag ggaaacacct actactgata gtatgccgcc attattacga caagctatcg
      541 aattatttga tcaccaaggt gcagagccag ccttcttatt cggccttgaa ttgatcatat
      601 gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc tgcaaacgac gaaaactacg
      661 ctttagtagc ttaataacac tgatagtgct agtgtagatc actactagag ccaggcatca
      721 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
      781 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat
      841 actagagtcc ctatcagtga tagagattga catccctatc agtgatagag atactgagca
      901 c
//