##gff-version 3 ##sequence-region BBa_Q03500 1 986 BBa_Q03500 . ribosome_entry_site 1 11 . + 0 ID=BBa_B0033;Name=BBa_B0033 BBa_Q03500 . engineered_region 1 11 . + 0 ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033 BBa_Q03500 . ribosome_entry_site 1 11 . + 0 ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033 BBa_Q03500 . ribosome_entry_site 7 10 . + 0 ID=annotation1714;Name=RBS;Parent=BBa_B0033 BBa_Q03500 . promoter 932 986 . + 0 ID=BBa_R0050;Name=BBa_R0050 BBa_Q03500 . ribosome_entry_site 944 958 . + 0 ID=annotation2013;Name=OR2;Parent=BBa_R0050 BBa_Q03500 . promoter 961 966 . + 0 ID=annotation2014;Name=-10;Parent=BBa_R0050 BBa_Q03500 . promoter 939 944 . + 0 ID=annotation2012;Name=-35;Parent=BBa_R0050 BBa_Q03500 . start_codon 974 974 . + 0 ID=annotation2018;Name=putative;Parent=BBa_R0050 BBa_Q03500 . engineered_region 932 986 . + 0 ID=annotation7066;Name=BBa_R0050;Parent=BBa_R0050 BBa_Q03500 . ribosome_entry_site 968 982 . + 0 ID=annotation2015;Name=OR1;Parent=BBa_R0050 BBa_Q03500 . terminator 883 923 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_Q03500 . polyA_site 910 923 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_Q03500 . stem_loop 890 909 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_Q03500 . engineered_region 883 923 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_Q03500 . stop_codon 916 916 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_Q03500 . terminator 795 874 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_Q03500 . stem_loop 806 849 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_Q03500 . engineered_region 795 874 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_Q03500 . CDS 18 761 . + 0 ID=BBa_C0050;Name=BBa_C0050 BBa_Q03500 . CDS 18 761 . + 0 ID=annotation1735;Name=cI HK022;Parent=BBa_C0050 BBa_Q03500 . engineered_tag 762 786 . + 0 ID=annotation2213990;Name=Help:Barcodes;Parent=BBa_C0050 BBa_Q03500 . non_covalent_binding_site 18 25 . + 0 ID=annotation1737;Name=OR3 partial;Parent=BBa_C0050 BBa_Q03500 . stop_codon 756 761 . + 0 ID=annotation1734;Name=2;Parent=BBa_C0050 BBa_Q03500 . engineered_region 18 761 . + 0 ID=annotation7033;Name=BBa_C0050;Parent=BBa_C0050 BBa_Q03500 . tag 723 755 . + 0 ID=annotation1736;Name=SsrA;Parent=BBa_C0050 >BBa_Q03500 tcacacaggactactagatggttcaacagaaagagcgtgaaactttctcgcagaggcttgcgctggcctgtgataaagc gggattacctttgcatggtaggcaggctgatttagctgtcaggcttaaggtcacaccaaaagccattagtaaatggttc aacggggagtcaataccaagaaaagacaagatggaatctctggcttcggtgctgggaactactgctgcatatctgcatg gctatgctgatgatgacggtatcacggtaaatcatctatcaagatcaaatgattattatcgtgttgatgtattggatgt tcaggcgagcgccgggccaggaaccatggtttccaatgaatttatagaaaagataagagcaattgaatatacgaccgag caggcaagaattttatttaatggaaggccacaggaaagcgtaaaagtcatcacggttcgcggtgacagcatggagggaa ccatcaatccgggagatgagatctttgttgatgtatccataacctgttttgatggcgatggcatttatgtgtttgtata cgggaaaacaatgcacgttaagcgcctgcaaatgcaaaagaacaggcttgccgtcatctctgacaatgccgcttatgat cgatggtacatagaagaaggtgaagaagagcaacttcacattctagccaaagtcctcattaggcagtcaatcgattaca agcgattcggagctgcaaacgacgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccctact agagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacg ctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttaggtattgactgtac tatcagttccgtcataatatgaaccataagttcaccac