LOCUS       BBa_Q04740               688 bp    DNA     linear       22-Dec-2024
DEFINITION  Pen1 quad part inverter
ACCESSION   BBa_Q04740
VERSION     BBa_Q04740.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..405
                     /label=penI
     misc_feature    403..435
                     /label=LVA
     misc_feature    442..466
                     /label=Help:Barcodes
     CDS             19..441
                     /label=BBa_C0074
     stem_loop       486..529
                     /label=stem_loop
     misc_feature    475..554
                     /label=BBa_B0010
     terminator      475..554
                     /label=BBa_B0010
     stem_loop       570..589
                     /label=T7 TE
     misc_feature    596..596
                     /label=stop
     polyA_site      590..603
                     /label=polya
     misc_feature    563..603
                     /label=BBa_B0012
     terminator      563..603
                     /label=BBa_B0012
     misc_feature    612..688
                     /label=PenI
     promoter        634..639
                     /label=-35
     promoter        657..662
                     /label=-10
     misc_feature    642..664
                     /label=dimer left half
     misc_feature    666..687
                     /label=dimer right half
     promoter        612..688
                     /label=BBa_R0074
ORIGIN
        1 aaagaggaga aatactagat gaaaaaaata ccacagattt ctgatgcgga actcgaagtc
       61 atgaaagtga tttggaagca ttcttccatt aatactaatg aggtaatcaa agagctcagt
      121 aaaacttcaa cgtggagccc aaaaactatt cagactatgc tgctgcgcct tatcaaaaaa
      181 ggcgctctca accaccataa agaaggtcgg gttttcgttt acacgcctaa tatagacgaa
      241 tcagattata tagaggttaa gtcacactca tttctcaacc ggttttacaa tggtacatta
      301 aattccatgg tactcaactt tttggagaat gatcaactgt cgggagaaga aatcaatgaa
      361 ttgtatcaga tactcgaaga acataagaac cgtaagaagg aagctgcaaa cgacgaaaac
      421 tacgctttag tagcttaata actctgatag tgctagtgta gatctctact agagccaggc
      481 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      541 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      601 atatactaga gtcatttcca accgaaaaaa ggcttgcatt taaatcttac atatgtaata
      661 ctttcaaaga ctacatttgt aagatttg
//