LOCUS       BBa_Q07400               910 bp    DNA     linear       22-Dec-2024
DEFINITION  tetR inverter, RBS3
ACCESSION   BBa_Q07400
VERSION     BBa_Q07400.1  
FEATURES             Location/Qualifiers
     RBS             1..20
                     /label=BBa_B0041
     CDS             30..646
                     /label=tetR
     misc_feature    647..680
                     /label=SsrA
     misc_feature    687..711
                     /label=Help:Barcodes
     CDS             27..686
                     /label=BBa_C0040
     stem_loop       731..774
                     /label=stem_loop
     misc_feature    720..799
                     /label=BBa_B0010
     terminator      720..799
                     /label=BBa_B0010
     stem_loop       815..834
                     /label=T7 TE
     misc_feature    841..841
                     /label=stop
     polyA_site      835..848
                     /label=polya
     misc_feature    808..848
                     /label=BBa_B0012
     terminator      808..848
                     /label=BBa_B0012
     misc_feature    857..875
                     /label=TetR 1
     misc_feature    857..910
                     /label=BBa_R0040
     promoter        876..881
                     /label=-35
     misc_feature    882..900
                     /label=TetR 2
     promoter        899..904
                     /label=-10
     promoter        857..910
                     /label=BBa_R0040
ORIGIN
        1 tcacacagga cggccggatg tactagatgt ccagattaga taaaagtaaa gtgattaaca
       61 gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt aaactcgccc
      121 agaagctagg tgtagagcag cctacattgt attggcatgt aaaaaataag cgggctttgc
      181 tcgacgcctt agccattgag atgttagata ggcaccatac tcacttttgc cctttagaag
      241 gggaaagctg gcaagatttt ttacgtaata acgctaaaag ttttagatgt gctttactaa
      301 gtcatcgcga tggagcaaaa gtacatttag gtacacggcc tacagaaaaa cagtatgaaa
      361 ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt ttcactagag aatgcattat
      421 atgcactcag cgctgtgggg cattttactt taggttgcgt attggaagat caagagcatc
      481 aagtcgctaa agaagaaagg gaaacaccta ctactgatag tatgccgcca ttattacgac
      541 aagctatcga attatttgat caccaaggtg cagagccagc cttcttattc ggccttgaat
      601 tgatcatatg cggattagaa aaacaactta aatgtgaaag tgggtccgct gcaaacgacg
      661 aaaactacgc tttagtagct taataacact gatagtgcta gtgtagatca ctactagagc
      721 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
      781 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
      841 cgtttatata ctagagtccc tatcagtgat agagattgac atccctatca gtgatagaga
      901 tactgagcac
//