LOCUS       BBa_Q08400               913 bp    DNA     linear       22-Dec-2024
DEFINITION  tetR inverter, RBS1
ACCESSION   BBa_Q08400
VERSION     BBa_Q08400.1  
FEATURES             Location/Qualifiers
     RBS             1..23
                     /label=BBa_B0038
     CDS             33..649
                     /label=tetR
     misc_feature    650..683
                     /label=SsrA
     misc_feature    690..714
                     /label=Help:Barcodes
     CDS             30..689
                     /label=BBa_C0040
     stem_loop       734..777
                     /label=stem_loop
     misc_feature    723..802
                     /label=BBa_B0010
     terminator      723..802
                     /label=BBa_B0010
     stem_loop       818..837
                     /label=T7 TE
     misc_feature    844..844
                     /label=stop
     polyA_site      838..851
                     /label=polya
     misc_feature    811..851
                     /label=BBa_B0012
     terminator      811..851
                     /label=BBa_B0012
     misc_feature    860..878
                     /label=TetR 1
     misc_feature    860..913
                     /label=BBa_R0040
     promoter        879..884
                     /label=-35
     misc_feature    885..903
                     /label=TetR 2
     promoter        902..907
                     /label=-10
     promoter        860..913
                     /label=BBa_R0040
ORIGIN
        1 tcacacagga aaccggttcg atgtactaga tgtccagatt agataaaagt aaagtgatta
       61 acagcgcatt agagctgctt aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg
      121 cccagaagct aggtgtagag cagcctacat tgtattggca tgtaaaaaat aagcgggctt
      181 tgctcgacgc cttagccatt gagatgttag ataggcacca tactcacttt tgccctttag
      241 aaggggaaag ctggcaagat tttttacgta ataacgctaa aagttttaga tgtgctttac
      301 taagtcatcg cgatggagca aaagtacatt taggtacacg gcctacagaa aaacagtatg
      361 aaactctcga aaatcaatta gcctttttat gccaacaagg tttttcacta gagaatgcat
      421 tatatgcact cagcgctgtg gggcatttta ctttaggttg cgtattggaa gatcaagagc
      481 atcaagtcgc taaagaagaa agggaaacac ctactactga tagtatgccg ccattattac
      541 gacaagctat cgaattattt gatcaccaag gtgcagagcc agccttctta ttcggccttg
      601 aattgatcat atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc gctgcaaacg
      661 acgaaaacta cgctttagta gcttaataac actgatagtg ctagtgtaga tcactactag
      721 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
      781 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
      841 ctgcgtttat atactagagt ccctatcagt gatagagatt gacatcccta tcagtgatag
      901 agatactgag cac
//