LOCUS       BBa_Q20050               478 bp    DNA     linear       22-Nov-2024
DEFINITION  Dimeric zinc finger based inverter
ACCESSION   BBa_Q20050
VERSION     BBa_Q20050.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=RBS-1\Strong
     RBS             8..12
                     /label=RBS
     misc_feature    1..15
                     /label=BBa_B0030
     RBS             1..15
                     /label=BBa_B0030
     CDS             22..288
                     /label=Zif268.GCN4
     misc_feature    25..111
                     /label=finger 2 of Zif268
     misc_feature    112..171
                     /label=finger 3 of Zif268
     misc_feature    22..24
                     /label=start codon
     misc_feature    172..186
                     /label=domain linker
     misc_feature    187..282
                     /label=GCN4 leucine zipper homodimerization domain
     misc_feature    283..288
                     /label=double stop codon
     CDS             22..288
                     /label=BBa_C2005
     stem_loop       308..351
                     /label=stem_loop
     misc_feature    297..376
                     /label=BBa_B0010
     terminator      297..376
                     /label=BBa_B0010
     stem_loop       392..411
                     /label=T7 TE
     misc_feature    418..418
                     /label=stop
     polyA_site      412..425
                     /label=polya
     misc_feature    385..425
                     /label=BBa_B0012
     terminator      385..425
                     /label=BBa_B0012
     promoter        443..448
                     /label=-35
     promoter        466..471
                     /label=-10
     misc_feature    453..466
                     /label=Zif23
     misc_feature    434..442
                     /label=consensus
     misc_feature    478..478
                     /label=(+1)
     promoter        434..478
                     /label=BBa_R2000
ORIGIN
        1 attaaagagg agaaatacta gatgaaaccg ttccagtgcc gtatctgcat gcgtaacttc
       61 tcccgttccg accacctgac cacccacatc cgtacccaca ccggtgaaaa accgttcgct
      121 tgcgacatct gcggtcgtaa attcgctcgt tccgacgaac gtaaacgtca ccgtaaattg
      181 cagcacatga aacagctgga agacaaagtt gaagaactgc tgtccaaaaa ctaccacctg
      241 gaaaacgaag ttgctcgtct gaaaaaactg gttggtgaac gttaataata ctagagccag
      301 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
      361 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
      421 ttatatacta gagagtttat tcttgacatg gtcccacgcg cgtgggatac tacgtcag
//