BBa_R0050
1
cI HK022
Promoter (HK022 cI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
Bacteriophage HK022.
The pR regulatory region is a 98 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two operator sites at which HK022 cI protein can bind cooperatively. The -35 and -10 regions from pR are included along with some flanking sequences. The cutoff for the 5' end of the regulatory region was placed just after the pM -10 region which promotes transcription of a gene upstream (thus the pM -10 region is not included). The cutoff for the 3' end of the regulatory region was placed immediately prior to the RBS. </p>
false
false
_1_
0
24
7
It's complicated
false
<P> <P>Derived from <genbank>STHK022N</genbank>.<P> Literature claims that there is no cross-talk between HK022 cI regulatory region, Lambda cI, 434 cI and P22 cI, but this has not been verified experimentally by the synthetic biology group at MIT.
false
Reshma Shetty
annotation2013
1
OR2
range2013
1
13
27
annotation2014
1
-10
range2014
1
30
35
annotation2012
1
-35
range2012
1
8
13
annotation2018
1
putative
range2018
1
43
43
annotation7066
1
BBa_R0050
range7066
1
1
55
annotation2015
1
OR1
range2015
1
37
51
BBa_R0050_sequence
1
ttaggtattgactgtactatcagttccgtcataatatgaaccataagttcaccac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z