BBa_B0037
1
BBa_B0037
Specialized RBS
2005-12-06T12:00:00Z
2015-08-31T04:07:20Z
Brink MF, Verbeet MP, de Boer HA (1995). Specialized ribosomes: highly specific translation in vivo of a single targetted mRNA species. Gene, 156, pp. 215-222
RBS specific to dedicated ribosomes. This RBS is a modified version of B0036. The spacing between the SD sequence and the start codon has been increased by 5 bases to give the same spacing as used by Brink et al. The 5 bases are the same as those used by Brink. That means they form the first five bases of an XbaI site and no negative affects of using these bases are known at present.
false
false
_11_6_
0
135
6
Not in stock
false
false
Barry Canton
BBa_R4037
1
BBa_R4037
Tet repressible promoter and dedicated RBS
2005-12-06T12:00:00Z
2015-05-08T01:14:16Z
This composite part is composed of a tet repressible promoter and a dedicated RBS. It will be used to construct a reporter system for VM1.0. GFP production from the reporter system can be compared to the GFP production of a similar system that uses a T7 promoter instead of an <I>E. coli</I> promoter and to I7102 which uses a slightly different dedicated RBS.
false
false
_11_6_
0
135
6
Not in stock
false
false
Barry Canton
component1760610
1
BBa_B0037
component1760605
1
BBa_R0040
annotation1760610
1
BBa_B0037
range1760610
1
63
72
annotation1760605
1
BBa_R0040
range1760605
1
1
54
BBa_R0040
1
p(tetR)
TetR repressible promoter
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210.
Released HQ 2013
Sequence for pTet inverting regulator driven by the TetR protein.</P>
false
true
_1_
0
24
7
In stock
false
<P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P>
true
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
annotation1986785
1
-35
range1986785
1
20
25
annotation1986786
1
TetR 2
range1986786
1
26
44
annotation1986784
1
BBa_R0040
range1986784
1
1
54
annotation1986787
1
-10
range1986787
1
43
48
annotation1986783
1
TetR 1
range1986783
1
1
19
BBa_R4037_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagaggtgtgtctag
BBa_R0040_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
BBa_B0037_sequence
1
gtgtgtctag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z