LOCUS       BBa_S00002              1220 bp    DNA     linear       02-May-2025
DEFINITION  testing composite part creation
ACCESSION   BBa_S00002
VERSION     BBa_S00002.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     CDS             138..225
                     /label=end of LacI coding region (inactive)
     misc_feature    138..337
                     /label=BBa_R0010
     misc_feature    226..263
                     /label=CAP binding site
     promoter        274..279
                     /label=-35
     promoter        298..303
                     /label=-10
     misc_feature    303..337
                     /label=LacI binding site
     misc_feature    310..310
                     /label=start
     promoter        138..337
                     /label=BBa_R0010
     misc_feature    350..353
                     /label=conserved
     RBS             346..357
                     /label=BBa_B0034
     CDS             364..1083
                     /label=GFP protein
     CDS             364..1083
                     /label=BBa_E0040
     stem_loop       1103..1146
                     /label=stem_loop
     misc_feature    1092..1171
                     /label=BBa_B0010
     terminator      1092..1171
                     /label=BBa_B0010
     stem_loop       1187..1206
                     /label=T7 TE
     misc_feature    1213..1213
                     /label=stop
     polyA_site      1207..1220
                     /label=polya
     misc_feature    1180..1220
                     /label=BBa_B0012
     terminator      1180..1220
                     /label=BBa_B0012
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagagcaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
      181 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
      241 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
      301 gttgtgtgga attgtgagcg gataacaatt tcacacatac tagagaaaga ggagaaatac
      361 tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
      421 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac
      481 ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca
      541 cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa
      601 cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt
      661 ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt
      721 gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac
      781 aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat
      841 ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca
      901 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat
      961 tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc
     1021 cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa
     1081 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1141 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     1201 tgggcctttc tgcgtttata
//