LOCUS       BBa_S01004              1905 bp    DNA     linear       01-May-2024
DEFINITION  E0432.R0062.E0422
ACCESSION   BBa_S01004
VERSION     BBa_S01004.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..780
                     /label=YFP (LVA)
     misc_feature    775..780
                     /label=2
     misc_feature    736..774
                     /label=SsrA
     misc_feature    260..260
                     /label=A (Q->L)
     misc_feature    19..780
                     /label=BBa_E0032
     misc_feature    19..780
                     /label=BBa_E0032
     stem_loop       800..843
                     /label=stem_loop
     misc_feature    789..868
                     /label=BBa_B0010
     terminator      789..868
                     /label=BBa_B0010
     stem_loop       884..903
                     /label=T7 TE
     misc_feature    910..910
                     /label=stop
     polyA_site      904..917
                     /label=polya
     misc_feature    877..917
                     /label=BBa_B0012
     terminator      877..917
                     /label=BBa_B0012
     misc_feature    926..945
                     /label=LuxR/HSL
     promoter        945..950
                     /label=-35
     promoter        967..972
                     /label=-10
     misc_feature    978..978
                     /label=start
     misc_feature    926..980
                     /label=BBa_R0062
     promoter        926..980
                     /label=BBa_R0062
     misc_feature    993..996
                     /label=conserved
     RBS             989..1000
                     /label=BBa_B0034
     CDS             1007..1768
                     /label=CFP (LVA)
     misc_feature    1725..1762
                     /label=SsrA
     misc_feature    1763..1768
                     /label=2
     misc_feature    1075..1075
                     /label=A
     misc_feature    1007..1768
                     /label=BBa_E0022
     misc_feature    1007..1768
                     /label=BBa_E0022
     stem_loop       1788..1831
                     /label=stem_loop
     misc_feature    1777..1856
                     /label=BBa_B0010
     terminator      1777..1856
                     /label=BBa_B0010
     stem_loop       1872..1891
                     /label=T7 TE
     misc_feature    1898..1898
                     /label=stop
     polyA_site      1892..1905
                     /label=polya
     misc_feature    1865..1905
                     /label=BBa_B0012
     terminator      1865..1905
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
       61 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
      121 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
      181 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgcaatg cttcgcccgc
      241 taccccgacc acatgaagct gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
      301 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
      361 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
      421 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
      481 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
      541 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
      601 ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag
      661 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
      721 gacgagctgt acaagaggcc tgctgcaaac gacgaaaact acgctttagt agcttaataa
      781 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
      841 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
      901 gcctttctgc gtttatatac tagagacctg taggatcgta caggtttacg caagaaaatg
      961 gtttgttata gtcgaataaa tactagagaa agaggagaaa tactagatgg tgagcaaggg
     1021 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtgaacgg
     1081 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     1141 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     1201 gacctggggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     1261 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     1321 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     1381 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     1441 ctacatcagc cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa
     1501 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     1561 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     1621 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     1681 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagaggcctg ctgcaaacga
     1741 cgaaaactac gctttagtag cttaataata ctagagccag gcatcaaata aaacgaaagg
     1801 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     1861 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//